Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04140

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04135 and BWI76_RS04140 are separated by 52 nucleotidesBWI76_RS04140 and BWI76_RS04145 are separated by 94 nucleotidesBWI76_RS04145 and BWI76_RS04150 are separated by 58 nucleotides BWI76_RS04135: BWI76_RS04135 - thymidine phosphorylase, at 850,425 to 851,747 _RS04135 BWI76_RS04140: BWI76_RS04140 - phosphopentomutase, at 851,800 to 853,023 _RS04140 BWI76_RS04145: BWI76_RS04145 - purine-nucleoside phosphorylase, at 853,118 to 853,840 _RS04145 BWI76_RS04150: BWI76_RS04150 - lipoate--protein ligase A, at 853,899 to 854,915 _RS04150 Position (kb) 851 852 853 854Strain fitness (log2 ratio) -1 0 1at 850.836 kb on + strand, within BWI76_RS04135at 851.062 kb on + strand, within BWI76_RS04135at 851.062 kb on + strand, within BWI76_RS04135at 851.077 kb on - strand, within BWI76_RS04135at 851.136 kb on + strandat 851.331 kb on + strand, within BWI76_RS04135at 851.339 kb on - strand, within BWI76_RS04135at 851.339 kb on - strand, within BWI76_RS04135at 851.661 kb on - strandat 851.663 kb on + strandat 851.752 kb on + strandat 851.755 kb on - strandat 851.760 kb on - strandat 851.896 kb on - strandat 851.972 kb on + strand, within BWI76_RS04140at 851.980 kb on - strand, within BWI76_RS04140at 851.990 kb on - strand, within BWI76_RS04140at 852.095 kb on + strand, within BWI76_RS04140at 852.195 kb on + strand, within BWI76_RS04140at 852.378 kb on + strand, within BWI76_RS04140at 852.585 kb on + strand, within BWI76_RS04140at 852.856 kb on + strand, within BWI76_RS04140at 852.866 kb on + strand, within BWI76_RS04140at 852.948 kb on - strandat 853.171 kb on + strandat 853.670 kb on + strand, within BWI76_RS04145at 853.696 kb on - strand, within BWI76_RS04145at 853.751 kb on + strand, within BWI76_RS04145at 853.789 kb on - strandat 853.815 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
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850,836 + BWI76_RS04135 0.31 -0.3
851,062 + BWI76_RS04135 0.48 -1.1
851,062 + BWI76_RS04135 0.48 +0.6
851,077 - BWI76_RS04135 0.49 +0.6
851,136 + -0.8
851,331 + BWI76_RS04135 0.68 -0.2
851,339 - BWI76_RS04135 0.69 +0.3
851,339 - BWI76_RS04135 0.69 +0.6
851,661 - -0.3
851,663 + -0.4
851,752 + -0.3
851,755 - -0.2
851,760 - +0.2
851,896 - +0.0
851,972 + BWI76_RS04140 0.14 +0.3
851,980 - BWI76_RS04140 0.15 +0.4
851,990 - BWI76_RS04140 0.16 -0.8
852,095 + BWI76_RS04140 0.24 +0.2
852,195 + BWI76_RS04140 0.32 -0.3
852,378 + BWI76_RS04140 0.47 -0.1
852,585 + BWI76_RS04140 0.64 -0.4
852,856 + BWI76_RS04140 0.86 +0.7
852,866 + BWI76_RS04140 0.87 +0.1
852,948 - -0.3
853,171 + +0.2
853,670 + BWI76_RS04145 0.76 -0.0
853,696 - BWI76_RS04145 0.80 -0.2
853,751 + BWI76_RS04145 0.88 +1.4
853,789 - -0.3
853,815 + -0.9

Or see this region's nucleotide sequence