Experiment: MoLS4 with L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptB and buk overlap by 4 nucleotides buk and DVU0629 are separated by 142 nucleotides DVU0629 and DVU0630 are separated by 3 nucleotides
DVU0627: ptB - phosphotransbutyrylase (TIGR), at 694,034 to 695,065
ptB
DVU0628: buk - butyrate kinase (TIGR), at 695,062 to 696,180
buk
DVU0629: DVU0629 - transcriptional regulator, TetR family (TIGR), at 696,323 to 696,883
DVU0629
DVU0630: DVU0630 - hypothetical protein (TIGR), at 696,887 to 697,063
DVU0630
Position (kb) 695
696
697 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 694.085 kb on - strand at 694.113 kb on + strand at 694.231 kb on - strand, within ptB at 694.294 kb on - strand, within ptB at 694.361 kb on + strand, within ptB at 694.414 kb on - strand, within ptB at 694.423 kb on + strand, within ptB at 694.454 kb on - strand, within ptB at 694.537 kb on + strand, within ptB at 694.593 kb on - strand, within ptB at 694.609 kb on + strand, within ptB at 694.647 kb on + strand, within ptB at 694.738 kb on - strand, within ptB at 694.797 kb on + strand, within ptB at 694.805 kb on - strand, within ptB at 694.839 kb on - strand, within ptB at 694.897 kb on + strand, within ptB at 695.151 kb on - strand at 695.242 kb on - strand, within buk at 695.259 kb on - strand, within buk at 695.308 kb on + strand, within buk at 695.396 kb on - strand, within buk at 695.422 kb on + strand, within buk at 695.460 kb on + strand, within buk at 695.614 kb on + strand, within buk at 695.891 kb on - strand, within buk at 695.922 kb on + strand, within buk at 695.965 kb on + strand, within buk at 695.965 kb on + strand, within buk at 696.005 kb on + strand, within buk at 696.074 kb on + strand at 696.082 kb on - strand at 696.127 kb on - strand at 696.130 kb on + strand at 696.152 kb on + strand at 696.160 kb on - strand at 696.169 kb on + strand at 696.194 kb on - strand at 696.194 kb on - strand at 696.194 kb on - strand at 696.201 kb on + strand at 696.248 kb on + strand at 696.256 kb on - strand at 696.280 kb on + strand at 696.445 kb on - strand, within DVU0629 at 696.451 kb on + strand, within DVU0629 at 696.459 kb on - strand, within DVU0629 at 696.462 kb on + strand, within DVU0629 at 696.633 kb on - strand, within DVU0629 at 696.649 kb on - strand, within DVU0629 at 696.716 kb on - strand, within DVU0629 at 696.863 kb on - strand at 696.916 kb on + strand, within DVU0630 at 696.919 kb on - strand, within DVU0630 at 697.012 kb on - strand, within DVU0630
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Isoleucine (N) remove 694,085 - -4.6 694,113 + -4.9 694,231 - ptB DVU0627 0.19 -2.8 694,294 - ptB DVU0627 0.25 -3.3 694,361 + ptB DVU0627 0.32 -2.3 694,414 - ptB DVU0627 0.37 -3.0 694,423 + ptB DVU0627 0.38 -2.5 694,454 - ptB DVU0627 0.41 -1.2 694,537 + ptB DVU0627 0.49 -3.0 694,593 - ptB DVU0627 0.54 -3.1 694,609 + ptB DVU0627 0.56 -2.0 694,647 + ptB DVU0627 0.59 -2.9 694,738 - ptB DVU0627 0.68 -3.5 694,797 + ptB DVU0627 0.74 -3.5 694,805 - ptB DVU0627 0.75 -3.6 694,839 - ptB DVU0627 0.78 -2.1 694,897 + ptB DVU0627 0.84 -3.1 695,151 - -0.9 695,242 - buk DVU0628 0.16 -1.1 695,259 - buk DVU0628 0.18 -0.8 695,308 + buk DVU0628 0.22 -1.6 695,396 - buk DVU0628 0.30 -1.0 695,422 + buk DVU0628 0.32 -1.4 695,460 + buk DVU0628 0.36 -0.9 695,614 + buk DVU0628 0.49 -1.4 695,891 - buk DVU0628 0.74 -1.3 695,922 + buk DVU0628 0.77 -1.1 695,965 + buk DVU0628 0.81 -0.2 695,965 + buk DVU0628 0.81 -0.5 696,005 + buk DVU0628 0.84 -1.9 696,074 + -0.3 696,082 - -1.1 696,127 - -0.5 696,130 + -1.6 696,152 + -0.5 696,160 - -2.1 696,169 + -0.4 696,194 - -0.1 696,194 - +0.1 696,194 - -0.3 696,201 + -0.8 696,248 + -1.8 696,256 - -0.8 696,280 + -0.6 696,445 - DVU0629 0.22 +0.0 696,451 + DVU0629 0.23 -0.0 696,459 - DVU0629 0.24 +0.9 696,462 + DVU0629 0.25 +0.3 696,633 - DVU0629 0.55 +0.2 696,649 - DVU0629 0.58 +0.1 696,716 - DVU0629 0.70 -0.0 696,863 - -0.2 696,916 + DVU0630 0.16 -0.0 696,919 - DVU0630 0.18 -0.7 697,012 - DVU0630 0.71 -0.6
Or see this region's nucleotide sequence