Experiment: LB - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SOA0167 and SOA0168 are separated by 163 nucleotides SOA0168 and SOA0169 overlap by 4 nucleotides SOA0169 and SOA0170 overlap by 7 nucleotides SOA0170 and SOA0171 are separated by 272 nucleotides SOA0171 and SOA0172 are separated by 0 nucleotides
SOA0167: SOA0167 - ISSod9, transposase (NCBI ptt file), at 155,302 to 158,268
SOA0167
SOA0168: SOA0168 - ISSo9, nucleotidyltransferase domain protein (NCBI ptt file), at 158,432 to 158,722
SOA0168
SOA0169: SOA0169 - ISSod9, conserved hypothetical protein (NCBI ptt file), at 158,719 to 159,096
SOA0169
SOA0170: SOA0170 - ISSod9, DNA-invertase (NCBI ptt file), at 159,090 to 159,647
SOA0170
SOA0171: SOA0171 - hypothetical protein (NCBI ptt file), at 159,920 to 160,177
SOA0171
SOA0172: SOA0172 - site-specific recombinase, resolvase family (NCBI ptt file), at 160,178 to 160,804
SOA0172
Position (kb) 159
160 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 159.687 kb on + strand at 159.699 kb on + strand at 159.700 kb on - strand at 159.700 kb on - strand at 159.709 kb on + strand at 159.709 kb on + strand at 159.709 kb on + strand at 159.709 kb on + strand at 159.778 kb on + strand at 159.778 kb on - strand at 159.783 kb on + strand at 159.791 kb on - strand at 159.791 kb on - strand at 159.813 kb on - strand at 159.840 kb on - strand at 160.098 kb on + strand, within SOA0171 at 160.106 kb on - strand, within SOA0171 at 160.106 kb on - strand, within SOA0171 at 160.125 kb on - strand, within SOA0171 at 160.126 kb on - strand, within SOA0171 at 160.175 kb on - strand at 160.242 kb on + strand, within SOA0172 at 160.291 kb on - strand, within SOA0172 at 160.322 kb on + strand, within SOA0172 at 160.322 kb on - strand, within SOA0172 at 160.383 kb on + strand, within SOA0172 at 160.451 kb on + strand, within SOA0172 at 160.451 kb on + strand, within SOA0172 at 160.459 kb on - strand, within SOA0172 at 160.467 kb on - strand, within SOA0172 at 160.564 kb on + strand, within SOA0172 at 160.564 kb on + strand, within SOA0172 at 160.564 kb on + strand, within SOA0172 at 160.572 kb on - strand, within SOA0172 at 160.572 kb on - strand, within SOA0172 at 160.572 kb on - strand, within SOA0172 at 160.574 kb on + strand, within SOA0172 at 160.582 kb on - strand, within SOA0172
Per-strain Table
Position Strand Gene LocusTag Fraction LB - no RNA protect remove 159,687 + -1.7 159,699 + -1.9 159,700 - -2.2 159,700 - -1.2 159,709 + -3.0 159,709 + -2.7 159,709 + -2.4 159,709 + -1.9 159,778 + -2.2 159,778 - -1.1 159,783 + -1.4 159,791 - -1.3 159,791 - -2.0 159,813 - -2.1 159,840 - -2.1 160,098 + SOA0171 0.69 -3.1 160,106 - SOA0171 0.72 -1.0 160,106 - SOA0171 0.72 -2.7 160,125 - SOA0171 0.79 -1.8 160,126 - SOA0171 0.80 -1.8 160,175 - -3.1 160,242 + SOA0172 0.10 -2.0 160,291 - SOA0172 0.18 -2.8 160,322 + SOA0172 0.23 -1.7 160,322 - SOA0172 0.23 -2.4 160,383 + SOA0172 0.33 -1.2 160,451 + SOA0172 0.44 -1.8 160,451 + SOA0172 0.44 -1.5 160,459 - SOA0172 0.45 -2.0 160,467 - SOA0172 0.46 -2.1 160,564 + SOA0172 0.62 -2.4 160,564 + SOA0172 0.62 -1.4 160,564 + SOA0172 0.62 -2.9 160,572 - SOA0172 0.63 -1.8 160,572 - SOA0172 0.63 -2.2 160,572 - SOA0172 0.63 -1.3 160,574 + SOA0172 0.63 -3.0 160,582 - SOA0172 0.64 -3.3
Or see this region's nucleotide sequence