Experiment: LB - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3162 and SO3163 are separated by 210 nucleotides SO3163 and SO3164 are separated by 293 nucleotides SO3164 and SO3165 are separated by 105 nucleotides SO3165 and SO3166 overlap by 4 nucleotides
SO3162: SO3162 - sensor histidine kinase (NCBI ptt file), at 3,298,460 to 3,299,821
SO3162
SO3163: SO3163 - lipoprotein (NCBI ptt file), at 3,300,032 to 3,300,484
SO3163
SO3164: SO3164 - conserved hypothetical protein (NCBI ptt file), at 3,300,778 to 3,301,545
SO3164
SO3165: SO3165 - conserved hypothetical protein (NCBI ptt file), at 3,301,651 to 3,302,070
SO3165
SO3166: SO3166 - conserved hypothetical protein (NCBI ptt file), at 3,302,067 to 3,302,468
SO3166
Position (kb)
3300
3301
3302 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3299.818 kb on + strand at 3299.843 kb on + strand at 3299.851 kb on - strand at 3299.851 kb on - strand at 3299.855 kb on + strand at 3299.889 kb on + strand at 3299.922 kb on - strand at 3299.922 kb on - strand at 3299.931 kb on + strand at 3299.939 kb on - strand at 3300.025 kb on - strand at 3300.074 kb on + strand at 3300.207 kb on - strand, within SO3163 at 3300.207 kb on - strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.209 kb on + strand, within SO3163 at 3300.217 kb on - strand, within SO3163 at 3300.217 kb on - strand, within SO3163 at 3300.253 kb on + strand, within SO3163 at 3300.253 kb on + strand, within SO3163 at 3300.260 kb on - strand, within SO3163 at 3300.462 kb on - strand at 3300.512 kb on + strand at 3300.856 kb on - strand, within SO3164 at 3300.980 kb on - strand, within SO3164 at 3300.980 kb on - strand, within SO3164 at 3301.177 kb on + strand, within SO3164 at 3301.196 kb on + strand, within SO3164 at 3301.283 kb on + strand, within SO3164 at 3301.539 kb on + strand at 3301.753 kb on - strand, within SO3165 at 3302.098 kb on + strand at 3302.110 kb on + strand, within SO3166 at 3302.110 kb on + strand, within SO3166 at 3302.118 kb on - strand, within SO3166 at 3302.203 kb on + strand, within SO3166 at 3302.289 kb on - strand, within SO3166 at 3302.326 kb on + strand, within SO3166 at 3302.453 kb on + strand at 3302.453 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB - no RNA protect remove 3,299,818 + +0.9 3,299,843 + +0.7 3,299,851 - -2.8 3,299,851 - -2.1 3,299,855 + -0.5 3,299,889 + +0.5 3,299,922 - -0.6 3,299,922 - -0.5 3,299,931 + -0.2 3,299,939 - +1.2 3,300,025 - +0.0 3,300,074 + -0.1 3,300,207 - SO3163 0.39 +0.8 3,300,207 - SO3163 0.39 +1.3 3,300,209 + SO3163 0.39 -0.4 3,300,209 + SO3163 0.39 -0.3 3,300,209 + SO3163 0.39 +0.0 3,300,209 + SO3163 0.39 -1.1 3,300,209 + SO3163 0.39 -0.5 3,300,209 + SO3163 0.39 +0.4 3,300,209 + SO3163 0.39 -0.9 3,300,217 - SO3163 0.41 -0.2 3,300,217 - SO3163 0.41 +0.0 3,300,253 + SO3163 0.49 +0.4 3,300,253 + SO3163 0.49 +0.3 3,300,260 - SO3163 0.50 +0.7 3,300,462 - -0.0 3,300,512 + -1.0 3,300,856 - SO3164 0.10 -0.8 3,300,980 - SO3164 0.26 -0.2 3,300,980 - SO3164 0.26 -0.3 3,301,177 + SO3164 0.52 -0.4 3,301,196 + SO3164 0.54 +0.5 3,301,283 + SO3164 0.66 -1.5 3,301,539 + -0.2 3,301,753 - SO3165 0.24 -1.1 3,302,098 + +0.4 3,302,110 + SO3166 0.11 -0.6 3,302,110 + SO3166 0.11 -0.5 3,302,118 - SO3166 0.13 -0.3 3,302,203 + SO3166 0.34 -0.4 3,302,289 - SO3166 0.55 -0.2 3,302,326 + SO3166 0.64 -0.3 3,302,453 + +0.3 3,302,453 + -0.5
Or see this region's nucleotide sequence