Experiment: LB - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiS and thiF overlap by 4 nucleotides thiF and thiDE overlap by 4 nucleotides thiDE and thiC overlap by 1 nucleotides
SO2442: thiS - thiS protein, putative (NCBI ptt file), at 2,555,858 to 2,556,061
thiS
SO2443: thiF - thiF protein, putative (NCBI ptt file), at 2,556,058 to 2,556,960
thiF
SO2444: thiDE - phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file), at 2,556,957 to 2,558,537
thiDE
SO2445: thiC - thiamin biosynthesis protein ThiC (NCBI ptt file), at 2,558,537 to 2,560,702
thiC
Position (kb)
2556
2557
2558
2559 Strain fitness (log2 ratio)
-1
0
1 at 2556.483 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.918 kb on - strand at 2556.971 kb on - strand at 2557.138 kb on - strand, within thiDE at 2557.138 kb on - strand, within thiDE at 2557.214 kb on - strand, within thiDE at 2557.280 kb on + strand, within thiDE at 2557.413 kb on - strand, within thiDE at 2557.483 kb on + strand, within thiDE at 2557.552 kb on + strand, within thiDE at 2557.555 kb on - strand, within thiDE at 2557.676 kb on + strand, within thiDE at 2557.690 kb on + strand, within thiDE at 2557.723 kb on + strand, within thiDE at 2557.729 kb on + strand, within thiDE at 2557.742 kb on + strand, within thiDE at 2557.760 kb on - strand, within thiDE at 2557.770 kb on + strand, within thiDE at 2557.778 kb on - strand, within thiDE at 2557.782 kb on + strand, within thiDE at 2557.790 kb on - strand, within thiDE at 2557.794 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2558.275 kb on - strand, within thiDE at 2558.316 kb on - strand, within thiDE at 2558.535 kb on + strand at 2558.543 kb on - strand at 2558.580 kb on + strand at 2558.613 kb on + strand at 2558.615 kb on - strand at 2558.863 kb on - strand, within thiC at 2558.898 kb on - strand, within thiC at 2558.905 kb on - strand, within thiC at 2559.185 kb on + strand, within thiC at 2559.193 kb on - strand, within thiC at 2559.193 kb on - strand, within thiC at 2559.278 kb on - strand, within thiC at 2559.302 kb on - strand, within thiC at 2559.362 kb on + strand, within thiC at 2559.408 kb on + strand, within thiC
Per-strain Table
Position Strand Gene LocusTag Fraction LB - no RNA protect remove 2,556,483 + thiF SO2443 0.47 -0.6 2,556,680 + thiF SO2443 0.69 +0.0 2,556,680 + thiF SO2443 0.69 +0.3 2,556,680 + thiF SO2443 0.69 -0.1 2,556,738 + thiF SO2443 0.75 +0.6 2,556,738 + thiF SO2443 0.75 -0.9 2,556,918 - +0.0 2,556,971 - -0.6 2,557,138 - thiDE SO2444 0.11 +0.6 2,557,138 - thiDE SO2444 0.11 +0.6 2,557,214 - thiDE SO2444 0.16 +0.6 2,557,280 + thiDE SO2444 0.20 +0.8 2,557,413 - thiDE SO2444 0.29 +0.7 2,557,483 + thiDE SO2444 0.33 +1.3 2,557,552 + thiDE SO2444 0.38 -0.1 2,557,555 - thiDE SO2444 0.38 +1.5 2,557,676 + thiDE SO2444 0.45 -0.1 2,557,690 + thiDE SO2444 0.46 -0.5 2,557,723 + thiDE SO2444 0.48 +0.5 2,557,729 + thiDE SO2444 0.49 +0.1 2,557,742 + thiDE SO2444 0.50 +0.1 2,557,760 - thiDE SO2444 0.51 +0.5 2,557,770 + thiDE SO2444 0.51 +0.5 2,557,778 - thiDE SO2444 0.52 +0.3 2,557,782 + thiDE SO2444 0.52 +0.3 2,557,790 - thiDE SO2444 0.53 +0.4 2,557,794 + thiDE SO2444 0.53 +1.4 2,557,874 + thiDE SO2444 0.58 -0.1 2,557,874 + thiDE SO2444 0.58 -0.2 2,558,275 - thiDE SO2444 0.83 +1.1 2,558,316 - thiDE SO2444 0.86 -0.9 2,558,535 + -0.9 2,558,543 - +0.4 2,558,580 + +1.3 2,558,613 + +0.1 2,558,615 - +1.2 2,558,863 - thiC SO2445 0.15 +0.3 2,558,898 - thiC SO2445 0.17 -0.7 2,558,905 - thiC SO2445 0.17 +0.3 2,559,185 + thiC SO2445 0.30 +0.4 2,559,193 - thiC SO2445 0.30 -1.2 2,559,193 - thiC SO2445 0.30 +0.1 2,559,278 - thiC SO2445 0.34 +0.8 2,559,302 - thiC SO2445 0.35 +1.1 2,559,362 + thiC SO2445 0.38 +0.6 2,559,408 + thiC SO2445 0.40 -0.1
Or see this region's nucleotide sequence