Experiment: LB - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1061 and def-2 overlap by 4 nucleotides def-2 and slyX are separated by 57 nucleotides slyX and SO1064 are separated by 9 nucleotides
SO1061: SO1061 - TPR domain protein (NCBI ptt file), at 1,100,866 to 1,102,266
SO1061
SO1062: def-2 - polypeptide deformylase (NCBI ptt file), at 1,102,263 to 1,102,808
def-2
SO1063: slyX - slyX protein (NCBI ptt file), at 1,102,866 to 1,103,078
slyX
SO1064: SO1064 - hypothetical WD domain protein (NCBI ptt file), at 1,103,088 to 1,104,038
SO1064
Position (kb)
1102
1103 Strain fitness (log2 ratio)
-2
-1
0
1 at 1101.271 kb on + strand, within SO1061 at 1101.455 kb on + strand, within SO1061 at 1101.542 kb on + strand, within SO1061 at 1101.600 kb on - strand, within SO1061 at 1101.696 kb on + strand, within SO1061 at 1101.723 kb on - strand, within SO1061 at 1101.753 kb on + strand, within SO1061 at 1101.758 kb on + strand, within SO1061 at 1101.907 kb on + strand, within SO1061 at 1101.940 kb on + strand, within SO1061 at 1102.103 kb on + strand, within SO1061 at 1102.108 kb on + strand, within SO1061 at 1102.117 kb on - strand, within SO1061 at 1102.152 kb on - strand at 1102.497 kb on - strand, within def-2 at 1102.505 kb on - strand, within def-2 at 1102.580 kb on + strand, within def-2 at 1102.887 kb on + strand at 1102.889 kb on + strand, within slyX at 1103.009 kb on - strand, within slyX at 1103.073 kb on + strand at 1103.085 kb on + strand at 1103.085 kb on + strand at 1103.190 kb on + strand, within SO1064 at 1103.199 kb on - strand, within SO1064 at 1103.263 kb on - strand, within SO1064 at 1103.411 kb on + strand, within SO1064 at 1103.433 kb on - strand, within SO1064 at 1103.435 kb on + strand, within SO1064 at 1103.437 kb on + strand, within SO1064 at 1103.443 kb on - strand, within SO1064 at 1103.443 kb on - strand, within SO1064 at 1103.499 kb on + strand, within SO1064 at 1103.529 kb on + strand, within SO1064 at 1103.529 kb on + strand, within SO1064 at 1103.549 kb on - strand, within SO1064 at 1103.627 kb on + strand, within SO1064 at 1103.627 kb on + strand, within SO1064 at 1103.650 kb on - strand, within SO1064 at 1103.662 kb on + strand, within SO1064 at 1103.709 kb on + strand, within SO1064 at 1103.748 kb on - strand, within SO1064
Per-strain Table
Position Strand Gene LocusTag Fraction LB - no RNA protect remove 1,101,271 + SO1061 0.29 -0.1 1,101,455 + SO1061 0.42 -0.5 1,101,542 + SO1061 0.48 -1.3 1,101,600 - SO1061 0.52 +0.7 1,101,696 + SO1061 0.59 -0.6 1,101,723 - SO1061 0.61 -0.1 1,101,753 + SO1061 0.63 -0.7 1,101,758 + SO1061 0.64 +1.1 1,101,907 + SO1061 0.74 -0.2 1,101,940 + SO1061 0.77 +0.1 1,102,103 + SO1061 0.88 -0.2 1,102,108 + SO1061 0.89 +0.2 1,102,117 - SO1061 0.89 +0.3 1,102,152 - +0.8 1,102,497 - def-2 SO1062 0.43 +0.2 1,102,505 - def-2 SO1062 0.44 +1.1 1,102,580 + def-2 SO1062 0.58 -1.0 1,102,887 + -0.3 1,102,889 + slyX SO1063 0.11 -0.9 1,103,009 - slyX SO1063 0.67 -0.3 1,103,073 + -0.0 1,103,085 + -0.5 1,103,085 + -0.1 1,103,190 + SO1064 0.11 +0.5 1,103,199 - SO1064 0.12 +0.4 1,103,263 - SO1064 0.18 -0.2 1,103,411 + SO1064 0.34 +0.0 1,103,433 - SO1064 0.36 -0.6 1,103,435 + SO1064 0.36 +0.1 1,103,437 + SO1064 0.37 +0.1 1,103,443 - SO1064 0.37 -0.0 1,103,443 - SO1064 0.37 +0.2 1,103,499 + SO1064 0.43 +0.1 1,103,529 + SO1064 0.46 -2.2 1,103,529 + SO1064 0.46 -0.4 1,103,549 - SO1064 0.48 +0.3 1,103,627 + SO1064 0.57 +1.3 1,103,627 + SO1064 0.57 -0.0 1,103,650 - SO1064 0.59 +0.1 1,103,662 + SO1064 0.60 +0.5 1,103,709 + SO1064 0.65 +0.7 1,103,748 - SO1064 0.69 -0.0
Or see this region's nucleotide sequence