Experiment: LB - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0684 and SO0685 are separated by 59 nucleotides SO0685 and SO0686 are separated by 118 nucleotides SO0686 and SO0687 overlap by 8 nucleotides SO0687 and SO0688 overlap by 4 nucleotides
SO0684: SO0684 - ISSod1, transposase OrfB (NCBI ptt file), at 703,881 to 704,690
SO0684
SO0685: SO0685 - ISSod1, transposase OrfA (NCBI ptt file), at 704,750 to 705,040
SO0685
SO0686: SO0686 - hypothetical protein (NCBI ptt file), at 705,159 to 705,623
SO0686
SO0687: SO0687 - hypothetical protein (NCBI ptt file), at 705,616 to 705,918
SO0687
SO0688: SO0688 - hypothetical protein (NCBI ptt file), at 705,915 to 707,714
SO0688
Position (kb) 705
706 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 705.093 kb on + strand at 705.093 kb on + strand at 705.093 kb on + strand at 705.093 kb on + strand at 705.093 kb on + strand at 705.093 kb on + strand at 705.093 kb on + strand at 705.110 kb on + strand at 705.110 kb on + strand at 705.110 kb on + strand at 705.116 kb on - strand at 705.116 kb on - strand at 705.116 kb on - strand at 705.118 kb on - strand at 705.213 kb on - strand, within SO0686 at 705.246 kb on + strand, within SO0686 at 705.257 kb on + strand, within SO0686 at 705.257 kb on + strand, within SO0686 at 705.265 kb on - strand, within SO0686 at 705.265 kb on - strand, within SO0686 at 705.265 kb on - strand, within SO0686 at 705.265 kb on - strand, within SO0686 at 705.467 kb on - strand, within SO0686 at 705.614 kb on - strand at 705.618 kb on + strand at 705.656 kb on - strand, within SO0687 at 705.678 kb on + strand, within SO0687 at 705.678 kb on + strand, within SO0687 at 705.678 kb on + strand, within SO0687 at 705.685 kb on + strand, within SO0687 at 705.685 kb on - strand, within SO0687 at 705.693 kb on - strand, within SO0687 at 705.695 kb on + strand, within SO0687 at 705.858 kb on + strand, within SO0687 at 705.866 kb on - strand, within SO0687 at 705.911 kb on + strand at 705.949 kb on + strand at 705.952 kb on - strand at 705.969 kb on + strand at 705.975 kb on - strand at 705.980 kb on - strand at 706.000 kb on - strand at 706.005 kb on - strand at 706.013 kb on - strand at 706.014 kb on - strand at 706.015 kb on - strand at 706.083 kb on - strand at 706.083 kb on - strand at 706.083 kb on - strand at 706.178 kb on + strand, within SO0688 at 706.208 kb on - strand, within SO0688 at 706.210 kb on + strand, within SO0688 at 706.234 kb on + strand, within SO0688 at 706.234 kb on + strand, within SO0688 at 706.242 kb on - strand, within SO0688 at 706.249 kb on + strand, within SO0688 at 706.251 kb on - strand, within SO0688 at 706.257 kb on + strand, within SO0688 at 706.257 kb on - strand, within SO0688 at 706.258 kb on - strand, within SO0688 at 706.391 kb on + strand, within SO0688 at 706.426 kb on - strand, within SO0688 at 706.426 kb on - strand, within SO0688 at 706.426 kb on - strand, within SO0688 at 706.588 kb on + strand, within SO0688 at 706.596 kb on - strand, within SO0688 at 706.596 kb on - strand, within SO0688 at 706.596 kb on - strand, within SO0688 at 706.642 kb on + strand, within SO0688 at 706.642 kb on + strand, within SO0688 at 706.642 kb on + strand, within SO0688 at 706.747 kb on - strand, within SO0688 at 706.854 kb on + strand, within SO0688
Per-strain Table
Position Strand Gene LocusTag Fraction LB - no RNA protect remove 705,093 + +0.8 705,093 + -0.7 705,093 + +1.0 705,093 + -1.3 705,093 + -0.5 705,093 + -1.1 705,093 + +0.6 705,110 + +0.4 705,110 + +0.8 705,110 + -0.4 705,116 - -0.0 705,116 - -1.1 705,116 - -1.6 705,118 - -2.8 705,213 - SO0686 0.12 -0.6 705,246 + SO0686 0.19 +0.3 705,257 + SO0686 0.21 +0.7 705,257 + SO0686 0.21 -0.4 705,265 - SO0686 0.23 +0.7 705,265 - SO0686 0.23 +0.4 705,265 - SO0686 0.23 +0.6 705,265 - SO0686 0.23 +0.3 705,467 - SO0686 0.66 +0.9 705,614 - +0.6 705,618 + +0.2 705,656 - SO0687 0.13 -0.4 705,678 + SO0687 0.20 -0.1 705,678 + SO0687 0.20 +0.1 705,678 + SO0687 0.20 -0.7 705,685 + SO0687 0.23 -0.1 705,685 - SO0687 0.23 -0.9 705,693 - SO0687 0.25 -0.2 705,695 + SO0687 0.26 -0.5 705,858 + SO0687 0.80 -0.1 705,866 - SO0687 0.83 -0.5 705,911 + +0.5 705,949 + -0.2 705,952 - -1.1 705,969 + -0.8 705,975 - -0.2 705,980 - +0.3 706,000 - -0.1 706,005 - +0.5 706,013 - -0.0 706,014 - -0.4 706,015 - -0.4 706,083 - -0.4 706,083 - -0.2 706,083 - -1.0 706,178 + SO0688 0.15 -0.1 706,208 - SO0688 0.16 -0.4 706,210 + SO0688 0.16 -1.5 706,234 + SO0688 0.18 +1.0 706,234 + SO0688 0.18 -0.4 706,242 - SO0688 0.18 +0.4 706,249 + SO0688 0.19 +0.4 706,251 - SO0688 0.19 -0.1 706,257 + SO0688 0.19 -0.9 706,257 - SO0688 0.19 +1.0 706,258 - SO0688 0.19 +0.2 706,391 + SO0688 0.26 +0.5 706,426 - SO0688 0.28 -0.2 706,426 - SO0688 0.28 +0.8 706,426 - SO0688 0.28 +0.4 706,588 + SO0688 0.37 +0.5 706,596 - SO0688 0.38 +0.5 706,596 - SO0688 0.38 +1.0 706,596 - SO0688 0.38 +1.1 706,642 + SO0688 0.40 +0.4 706,642 + SO0688 0.40 +2.7 706,642 + SO0688 0.40 -0.5 706,747 - SO0688 0.46 -0.4 706,854 + SO0688 0.52 +0.3
Or see this region's nucleotide sequence