Experiment: LB - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dcp-1 and SO_3143 are separated by 383 nucleotides SO_3143 and SO3142.2 overlap by 4 nucleotides SO3142.2 and SO3142.3 are separated by 63 nucleotides
SO3142: dcp-1 - peptidyl-dipeptidase Dcp (NCBI ptt file), at 3,276,288 to 3,278,438
dcp-1
SO_3143: SO_3143 - no description, at 3,278,822 to 3,279,541
_3143
SO3142.2: SO3142.2 - hypothetical Na+/H+ antiporte (NCBI ptt file), at 3,279,538 to 3,279,954
SO3142.2
SO3142.3: SO3142.3 - hypothetical Na+/H+ antiporte (NCBI ptt file), at 3,280,018 to 3,280,380
SO3142.3
Position (kb)
3278
3279
3280 Strain fitness (log2 ratio)
-1
0
1 at 3277.904 kb on + strand, within dcp-1 at 3277.904 kb on + strand, within dcp-1 at 3277.926 kb on - strand, within dcp-1 at 3277.957 kb on - strand, within dcp-1 at 3278.036 kb on + strand, within dcp-1 at 3278.102 kb on - strand, within dcp-1 at 3278.140 kb on + strand, within dcp-1 at 3278.148 kb on - strand, within dcp-1 at 3278.160 kb on - strand, within dcp-1 at 3278.192 kb on + strand, within dcp-1 at 3278.229 kb on + strand at 3278.286 kb on + strand at 3278.286 kb on + strand at 3278.294 kb on - strand at 3278.344 kb on + strand at 3278.388 kb on - strand at 3278.509 kb on + strand at 3278.777 kb on + strand at 3278.852 kb on + strand at 3278.923 kb on + strand, within SO_3143 at 3278.931 kb on - strand, within SO_3143 at 3278.959 kb on + strand, within SO_3143 at 3279.082 kb on - strand, within SO_3143 at 3279.148 kb on + strand, within SO_3143 at 3279.248 kb on - strand, within SO_3143 at 3279.323 kb on + strand, within SO_3143 at 3279.340 kb on + strand, within SO_3143 at 3279.356 kb on + strand, within SO_3143 at 3279.361 kb on - strand, within SO_3143 at 3279.366 kb on + strand, within SO_3143 at 3279.366 kb on + strand, within SO_3143 at 3279.366 kb on + strand, within SO_3143 at 3279.369 kb on - strand, within SO_3143 at 3279.374 kb on - strand, within SO_3143 at 3279.374 kb on - strand, within SO_3143 at 3279.374 kb on - strand, within SO_3143 at 3279.374 kb on - strand, within SO_3143 at 3279.383 kb on - strand, within SO_3143 at 3279.393 kb on + strand, within SO_3143 at 3279.405 kb on - strand, within SO_3143 at 3279.410 kb on - strand, within SO_3143 at 3279.518 kb on + strand at 3279.518 kb on + strand at 3279.526 kb on - strand at 3279.526 kb on - strand at 3279.526 kb on - strand at 3279.586 kb on + strand, within SO3142.2 at 3279.607 kb on + strand, within SO3142.2 at 3279.638 kb on + strand, within SO3142.2 at 3279.638 kb on + strand, within SO3142.2 at 3279.638 kb on + strand, within SO3142.2 at 3279.646 kb on - strand, within SO3142.2 at 3279.812 kb on - strand, within SO3142.2 at 3279.819 kb on - strand, within SO3142.2 at 3279.880 kb on - strand, within SO3142.2 at 3279.950 kb on + strand at 3280.102 kb on - strand, within SO3142.3 at 3280.223 kb on + strand, within SO3142.3 at 3280.223 kb on + strand, within SO3142.3 at 3280.231 kb on - strand, within SO3142.3 at 3280.250 kb on + strand, within SO3142.3 at 3280.368 kb on + strand at 3280.414 kb on + strand at 3280.473 kb on - strand at 3280.475 kb on + strand at 3280.477 kb on + strand at 3280.520 kb on + strand at 3280.539 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB - no RNA protect remove 3,277,904 + dcp-1 SO3142 0.75 +0.2 3,277,904 + dcp-1 SO3142 0.75 +1.2 3,277,926 - dcp-1 SO3142 0.76 -0.5 3,277,957 - dcp-1 SO3142 0.78 -0.4 3,278,036 + dcp-1 SO3142 0.81 +0.6 3,278,102 - dcp-1 SO3142 0.84 -0.7 3,278,140 + dcp-1 SO3142 0.86 +0.1 3,278,148 - dcp-1 SO3142 0.86 +0.2 3,278,160 - dcp-1 SO3142 0.87 +0.9 3,278,192 + dcp-1 SO3142 0.89 +0.0 3,278,229 + +1.4 3,278,286 + -0.0 3,278,286 + +0.7 3,278,294 - -0.6 3,278,344 + -0.6 3,278,388 - +0.3 3,278,509 + +0.2 3,278,777 + +0.3 3,278,852 + -0.0 3,278,923 + SO_3143 0.14 -0.2 3,278,931 - SO_3143 0.15 +0.7 3,278,959 + SO_3143 0.19 -0.1 3,279,082 - SO_3143 0.36 -0.1 3,279,148 + SO_3143 0.45 +0.8 3,279,248 - SO_3143 0.59 -0.6 3,279,323 + SO_3143 0.70 -0.3 3,279,340 + SO_3143 0.72 +1.2 3,279,356 + SO_3143 0.74 +0.2 3,279,361 - SO_3143 0.75 -1.7 3,279,366 + SO_3143 0.76 -1.0 3,279,366 + SO_3143 0.76 -0.1 3,279,366 + SO_3143 0.76 +0.3 3,279,369 - SO_3143 0.76 -0.0 3,279,374 - SO_3143 0.77 +0.1 3,279,374 - SO_3143 0.77 -0.3 3,279,374 - SO_3143 0.77 +0.2 3,279,374 - SO_3143 0.77 +0.3 3,279,383 - SO_3143 0.78 -1.1 3,279,393 + SO_3143 0.79 -1.1 3,279,405 - SO_3143 0.81 +0.2 3,279,410 - SO_3143 0.82 -0.9 3,279,518 + -1.7 3,279,518 + +0.1 3,279,526 - -0.1 3,279,526 - +0.1 3,279,526 - +0.4 3,279,586 + SO3142.2 0.12 +0.6 3,279,607 + SO3142.2 0.17 +1.4 3,279,638 + SO3142.2 0.24 +0.6 3,279,638 + SO3142.2 0.24 -1.0 3,279,638 + SO3142.2 0.24 +0.5 3,279,646 - SO3142.2 0.26 -0.8 3,279,812 - SO3142.2 0.66 +1.2 3,279,819 - SO3142.2 0.67 -0.0 3,279,880 - SO3142.2 0.82 +0.2 3,279,950 + -1.1 3,280,102 - SO3142.3 0.23 -1.1 3,280,223 + SO3142.3 0.56 +0.4 3,280,223 + SO3142.3 0.56 +0.4 3,280,231 - SO3142.3 0.59 +0.2 3,280,250 + SO3142.3 0.64 +0.7 3,280,368 + -0.6 3,280,414 + -0.4 3,280,473 - -1.1 3,280,475 + -1.0 3,280,477 + -0.1 3,280,520 + -0.2 3,280,539 - +0.6
Or see this region's nucleotide sequence