Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c18620 and PGA1_c18630 are separated by 478 nucleotides PGA1_c18630 and PGA1_c18640 are separated by 47 nucleotides PGA1_c18640 and PGA1_c18650 are separated by 302 nucleotides PGA1_c18650 and PGA1_c18660 are separated by 14 nucleotides PGA1_c18660 and PGA1_c18670 are separated by 40 nucleotides
PGA1_c18620: PGA1_c18620 - hypothetical protein, at 1,935,692 to 1,935,934
_c18620
PGA1_c18630: PGA1_c18630 - hypothetical protein, at 1,936,413 to 1,936,643
_c18630
PGA1_c18640: PGA1_c18640 - hypothetical protein, at 1,936,691 to 1,936,915
_c18640
PGA1_c18650: PGA1_c18650 - hypothetical protein, at 1,937,218 to 1,937,568
_c18650
PGA1_c18660: PGA1_c18660 - hypothetical protein, at 1,937,583 to 1,937,873
_c18660
PGA1_c18670: PGA1_c18670 - hypothetical protein, at 1,937,914 to 1,938,084
_c18670
Position (kb)
1936
1937 Strain fitness (log2 ratio)
-2
-1
0
1 at 1935.709 kb on + strand at 1935.709 kb on + strand at 1935.717 kb on - strand, within PGA1_c18620 at 1935.717 kb on - strand, within PGA1_c18620 at 1935.717 kb on - strand, within PGA1_c18620 at 1935.717 kb on - strand, within PGA1_c18620 at 1935.717 kb on - strand, within PGA1_c18620 at 1935.728 kb on + strand, within PGA1_c18620 at 1935.743 kb on - strand, within PGA1_c18620 at 1935.753 kb on - strand, within PGA1_c18620 at 1935.778 kb on - strand, within PGA1_c18620 at 1935.778 kb on - strand, within PGA1_c18620 at 1935.802 kb on + strand, within PGA1_c18620 at 1935.802 kb on + strand, within PGA1_c18620 at 1935.810 kb on - strand, within PGA1_c18620 at 1935.817 kb on - strand, within PGA1_c18620 at 1935.962 kb on - strand at 1936.002 kb on + strand at 1936.006 kb on + strand at 1936.042 kb on + strand at 1936.109 kb on + strand at 1936.109 kb on + strand at 1936.109 kb on + strand at 1936.109 kb on + strand at 1936.117 kb on - strand at 1936.117 kb on - strand at 1936.117 kb on - strand at 1936.117 kb on - strand at 1936.117 kb on - strand at 1936.127 kb on - strand at 1936.152 kb on - strand at 1936.168 kb on - strand at 1936.174 kb on - strand at 1936.237 kb on + strand at 1936.238 kb on + strand at 1936.297 kb on + strand at 1936.369 kb on - strand at 1936.410 kb on - strand at 1936.415 kb on - strand at 1936.420 kb on + strand at 1936.428 kb on - strand at 1936.449 kb on + strand, within PGA1_c18630 at 1936.449 kb on + strand, within PGA1_c18630 at 1936.474 kb on - strand, within PGA1_c18630 at 1936.476 kb on + strand, within PGA1_c18630 at 1936.558 kb on - strand, within PGA1_c18630 at 1936.580 kb on + strand, within PGA1_c18630 at 1936.659 kb on + strand at 1936.699 kb on + strand at 1936.742 kb on + strand, within PGA1_c18640 at 1936.743 kb on + strand, within PGA1_c18640 at 1936.743 kb on + strand, within PGA1_c18640 at 1936.802 kb on - strand, within PGA1_c18640 at 1936.844 kb on + strand, within PGA1_c18640 at 1936.844 kb on + strand, within PGA1_c18640 at 1936.846 kb on - strand, within PGA1_c18640 at 1936.852 kb on - strand, within PGA1_c18640 at 1936.954 kb on + strand at 1936.957 kb on - strand at 1936.957 kb on - strand at 1936.995 kb on - strand at 1937.166 kb on + strand at 1937.186 kb on - strand at 1937.198 kb on + strand at 1937.206 kb on - strand at 1937.213 kb on + strand at 1937.228 kb on + strand at 1937.242 kb on - strand at 1937.249 kb on + strand at 1937.268 kb on + strand, within PGA1_c18650 at 1937.268 kb on + strand, within PGA1_c18650 at 1937.268 kb on + strand, within PGA1_c18650 at 1937.268 kb on + strand, within PGA1_c18650 at 1937.331 kb on - strand, within PGA1_c18650 at 1937.367 kb on + strand, within PGA1_c18650 at 1937.429 kb on + strand, within PGA1_c18650 at 1937.429 kb on + strand, within PGA1_c18650 at 1937.429 kb on + strand, within PGA1_c18650 at 1937.429 kb on + strand, within PGA1_c18650 at 1937.430 kb on - strand, within PGA1_c18650 at 1937.431 kb on + strand, within PGA1_c18650 at 1937.437 kb on - strand, within PGA1_c18650 at 1937.446 kb on + strand, within PGA1_c18650 at 1937.605 kb on + strand at 1937.615 kb on + strand, within PGA1_c18660 at 1937.637 kb on + strand, within PGA1_c18660 at 1937.687 kb on + strand, within PGA1_c18660 at 1937.691 kb on + strand, within PGA1_c18660 at 1937.693 kb on + strand, within PGA1_c18660 at 1937.699 kb on - strand, within PGA1_c18660 at 1937.701 kb on + strand, within PGA1_c18660 at 1937.770 kb on + strand, within PGA1_c18660 at 1937.784 kb on + strand, within PGA1_c18660 at 1937.803 kb on + strand, within PGA1_c18660 at 1937.803 kb on + strand, within PGA1_c18660 at 1937.840 kb on + strand, within PGA1_c18660 at 1937.901 kb on + strand at 1937.901 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 1,935,709 + +0.2 1,935,709 + -0.4 1,935,717 - PGA1_c18620 0.10 -0.2 1,935,717 - PGA1_c18620 0.10 +0.4 1,935,717 - PGA1_c18620 0.10 +0.1 1,935,717 - PGA1_c18620 0.10 -0.7 1,935,717 - PGA1_c18620 0.10 -0.7 1,935,728 + PGA1_c18620 0.15 +0.3 1,935,743 - PGA1_c18620 0.21 +0.8 1,935,753 - PGA1_c18620 0.25 +0.2 1,935,778 - PGA1_c18620 0.35 +0.7 1,935,778 - PGA1_c18620 0.35 +0.3 1,935,802 + PGA1_c18620 0.45 +0.4 1,935,802 + PGA1_c18620 0.45 -1.5 1,935,810 - PGA1_c18620 0.49 -1.0 1,935,817 - PGA1_c18620 0.51 +0.3 1,935,962 - +0.0 1,936,002 + -1.9 1,936,006 + -0.5 1,936,042 + +0.6 1,936,109 + +0.0 1,936,109 + +0.6 1,936,109 + +0.6 1,936,109 + +1.1 1,936,117 - +0.2 1,936,117 - -0.4 1,936,117 - -0.8 1,936,117 - -0.5 1,936,117 - -0.7 1,936,127 - -0.5 1,936,152 - -0.2 1,936,168 - +0.4 1,936,174 - -0.5 1,936,237 + -0.3 1,936,238 + -0.2 1,936,297 + -0.4 1,936,369 - +0.2 1,936,410 - +1.7 1,936,415 - +0.2 1,936,420 + +0.3 1,936,428 - +0.9 1,936,449 + PGA1_c18630 0.16 +0.8 1,936,449 + PGA1_c18630 0.16 -0.1 1,936,474 - PGA1_c18630 0.26 -0.1 1,936,476 + PGA1_c18630 0.27 -0.9 1,936,558 - PGA1_c18630 0.63 +0.1 1,936,580 + PGA1_c18630 0.72 +0.1 1,936,659 + +0.1 1,936,699 + -0.4 1,936,742 + PGA1_c18640 0.23 -0.7 1,936,743 + PGA1_c18640 0.23 +0.2 1,936,743 + PGA1_c18640 0.23 -1.8 1,936,802 - PGA1_c18640 0.49 -0.1 1,936,844 + PGA1_c18640 0.68 -2.1 1,936,844 + PGA1_c18640 0.68 -1.2 1,936,846 - PGA1_c18640 0.69 -0.3 1,936,852 - PGA1_c18640 0.72 +0.1 1,936,954 + -1.0 1,936,957 - +0.6 1,936,957 - -0.6 1,936,995 - -2.1 1,937,166 + -0.3 1,937,186 - -1.9 1,937,198 + +0.6 1,937,206 - -0.6 1,937,213 + +0.3 1,937,228 + +0.4 1,937,242 - -0.9 1,937,249 + +0.5 1,937,268 + PGA1_c18650 0.14 -0.4 1,937,268 + PGA1_c18650 0.14 -1.2 1,937,268 + PGA1_c18650 0.14 +0.3 1,937,268 + PGA1_c18650 0.14 +0.2 1,937,331 - PGA1_c18650 0.32 +0.1 1,937,367 + PGA1_c18650 0.42 +1.1 1,937,429 + PGA1_c18650 0.60 -0.1 1,937,429 + PGA1_c18650 0.60 +0.7 1,937,429 + PGA1_c18650 0.60 -0.6 1,937,429 + PGA1_c18650 0.60 -0.1 1,937,430 - PGA1_c18650 0.60 -0.6 1,937,431 + PGA1_c18650 0.61 +0.7 1,937,437 - PGA1_c18650 0.62 +0.3 1,937,446 + PGA1_c18650 0.65 -0.4 1,937,605 + +0.2 1,937,615 + PGA1_c18660 0.11 +0.2 1,937,637 + PGA1_c18660 0.19 +0.2 1,937,687 + PGA1_c18660 0.36 +0.4 1,937,691 + PGA1_c18660 0.37 +1.1 1,937,693 + PGA1_c18660 0.38 -0.2 1,937,699 - PGA1_c18660 0.40 -0.2 1,937,701 + PGA1_c18660 0.41 -0.1 1,937,770 + PGA1_c18660 0.64 +0.9 1,937,784 + PGA1_c18660 0.69 -0.8 1,937,803 + PGA1_c18660 0.76 -0.5 1,937,803 + PGA1_c18660 0.76 +0.2 1,937,840 + PGA1_c18660 0.88 -1.0 1,937,901 + -0.1 1,937,901 + -0.5
Or see this region's nucleotide sequence