Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS18310

Experiment: Sebacic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS18300 and ABZR87_RS18305 are separated by 23 nucleotidesABZR87_RS18305 and ABZR87_RS18310 are separated by 72 nucleotidesABZR87_RS18310 and ABZR87_RS18315 are separated by 96 nucleotidesABZR87_RS18315 and ABZR87_RS18320 overlap by 14 nucleotides ABZR87_RS18300: ABZR87_RS18300 - YbhB/YbcL family Raf kinase inhibitor-like protein, at 474,015 to 474,578 _RS18300 ABZR87_RS18305: ABZR87_RS18305 - substrate-binding domain-containing protein, at 474,602 to 475,384 _RS18305 ABZR87_RS18310: ABZR87_RS18310 - sulfonate ABC transporter substrate-binding protein, at 475,457 to 476,449 _RS18310 ABZR87_RS18315: ABZR87_RS18315 - DUF971 domain-containing protein, at 476,546 to 476,836 _RS18315 ABZR87_RS18320: ABZR87_RS18320 - HEAT repeat domain-containing protein, at 476,823 to 477,782 _RS18320 Position (kb) 475 476 477Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 474.533 kb on + strandat 474.533 kb on + strandat 474.533 kb on + strandat 474.533 kb on + strandat 474.533 kb on + strandat 474.534 kb on - strandat 474.534 kb on - strandat 474.603 kb on + strandat 474.729 kb on + strand, within ABZR87_RS18305at 474.729 kb on + strand, within ABZR87_RS18305at 474.730 kb on - strand, within ABZR87_RS18305at 474.738 kb on + strand, within ABZR87_RS18305at 474.739 kb on - strand, within ABZR87_RS18305at 474.771 kb on + strand, within ABZR87_RS18305at 474.771 kb on + strand, within ABZR87_RS18305at 474.772 kb on - strand, within ABZR87_RS18305at 474.927 kb on - strand, within ABZR87_RS18305at 474.930 kb on + strand, within ABZR87_RS18305at 474.930 kb on + strand, within ABZR87_RS18305at 474.930 kb on + strand, within ABZR87_RS18305at 474.930 kb on + strand, within ABZR87_RS18305at 474.930 kb on + strand, within ABZR87_RS18305at 474.930 kb on + strand, within ABZR87_RS18305at 474.966 kb on + strand, within ABZR87_RS18305at 474.966 kb on + strand, within ABZR87_RS18305at 474.966 kb on + strand, within ABZR87_RS18305at 474.967 kb on - strand, within ABZR87_RS18305at 474.967 kb on - strand, within ABZR87_RS18305at 475.021 kb on + strand, within ABZR87_RS18305at 475.022 kb on - strand, within ABZR87_RS18305at 475.099 kb on - strand, within ABZR87_RS18305at 475.399 kb on + strandat 475.400 kb on - strandat 475.400 kb on - strandat 475.419 kb on + strandat 475.419 kb on + strandat 475.419 kb on + strandat 475.420 kb on - strandat 475.420 kb on - strandat 475.777 kb on - strand, within ABZR87_RS18310at 475.849 kb on - strand, within ABZR87_RS18310at 475.852 kb on - strand, within ABZR87_RS18310at 475.852 kb on - strand, within ABZR87_RS18310at 475.852 kb on - strand, within ABZR87_RS18310at 475.852 kb on - strand, within ABZR87_RS18310at 475.923 kb on + strand, within ABZR87_RS18310at 475.923 kb on + strand, within ABZR87_RS18310at 475.983 kb on + strand, within ABZR87_RS18310at 475.984 kb on - strand, within ABZR87_RS18310at 476.109 kb on + strand, within ABZR87_RS18310at 476.109 kb on + strand, within ABZR87_RS18310at 476.109 kb on + strand, within ABZR87_RS18310at 476.110 kb on - strand, within ABZR87_RS18310at 476.110 kb on - strand, within ABZR87_RS18310at 476.110 kb on - strand, within ABZR87_RS18310at 476.110 kb on - strand, within ABZR87_RS18310at 476.141 kb on + strand, within ABZR87_RS18310at 476.164 kb on - strand, within ABZR87_RS18310at 476.241 kb on + strand, within ABZR87_RS18310at 476.241 kb on + strand, within ABZR87_RS18310at 476.242 kb on - strand, within ABZR87_RS18310at 476.428 kb on + strandat 476.487 kb on + strandat 476.487 kb on + strandat 476.532 kb on + strandat 476.532 kb on + strandat 476.558 kb on + strandat 476.558 kb on + strandat 476.558 kb on + strandat 476.592 kb on + strand, within ABZR87_RS18315at 476.592 kb on + strand, within ABZR87_RS18315at 476.592 kb on + strand, within ABZR87_RS18315at 476.593 kb on - strand, within ABZR87_RS18315at 477.077 kb on - strand, within ABZR87_RS18320at 477.077 kb on - strand, within ABZR87_RS18320

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Per-strain Table

Position Strand Gene LocusTag Fraction Sebacic (C)
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474,533 + -2.9
474,533 + +0.5
474,533 + +0.7
474,533 + -1.9
474,533 + -3.0
474,534 - +2.1
474,534 - +1.9
474,603 + +3.4
474,729 + ABZR87_RS18305 0.16 -1.9
474,729 + ABZR87_RS18305 0.16 -2.9
474,730 - ABZR87_RS18305 0.16 +0.1
474,738 + ABZR87_RS18305 0.17 -0.8
474,739 - ABZR87_RS18305 0.17 +3.1
474,771 + ABZR87_RS18305 0.22 +0.5
474,771 + ABZR87_RS18305 0.22 +0.6
474,772 - ABZR87_RS18305 0.22 +0.1
474,927 - ABZR87_RS18305 0.42 -2.8
474,930 + ABZR87_RS18305 0.42 -1.3
474,930 + ABZR87_RS18305 0.42 +1.6
474,930 + ABZR87_RS18305 0.42 -4.6
474,930 + ABZR87_RS18305 0.42 +3.6
474,930 + ABZR87_RS18305 0.42 +0.6
474,930 + ABZR87_RS18305 0.42 -0.4
474,966 + ABZR87_RS18305 0.46 +1.7
474,966 + ABZR87_RS18305 0.46 -3.6
474,966 + ABZR87_RS18305 0.46 -2.8
474,967 - ABZR87_RS18305 0.47 +0.3
474,967 - ABZR87_RS18305 0.47 +0.7
475,021 + ABZR87_RS18305 0.54 +2.5
475,022 - ABZR87_RS18305 0.54 -2.9
475,099 - ABZR87_RS18305 0.63 +2.3
475,399 + -1.7
475,400 - +1.2
475,400 - -2.8
475,419 + -3.5
475,419 + -0.8
475,419 + -2.7
475,420 - -0.8
475,420 - -3.0
475,777 - ABZR87_RS18310 0.32 -0.6
475,849 - ABZR87_RS18310 0.39 -2.1
475,852 - ABZR87_RS18310 0.40 +0.6
475,852 - ABZR87_RS18310 0.40 +0.1
475,852 - ABZR87_RS18310 0.40 +1.7
475,852 - ABZR87_RS18310 0.40 -0.3
475,923 + ABZR87_RS18310 0.47 -1.2
475,923 + ABZR87_RS18310 0.47 +1.6
475,983 + ABZR87_RS18310 0.53 +0.7
475,984 - ABZR87_RS18310 0.53 +1.5
476,109 + ABZR87_RS18310 0.66 +1.1
476,109 + ABZR87_RS18310 0.66 -0.0
476,109 + ABZR87_RS18310 0.66 +0.5
476,110 - ABZR87_RS18310 0.66 -4.5
476,110 - ABZR87_RS18310 0.66 +2.0
476,110 - ABZR87_RS18310 0.66 -0.0
476,110 - ABZR87_RS18310 0.66 +0.8
476,141 + ABZR87_RS18310 0.69 +0.5
476,164 - ABZR87_RS18310 0.71 -3.7
476,241 + ABZR87_RS18310 0.79 -4.1
476,241 + ABZR87_RS18310 0.79 +0.9
476,242 - ABZR87_RS18310 0.79 +1.5
476,428 + -1.7
476,487 + +1.3
476,487 + +0.5
476,532 + -2.1
476,532 + -3.5
476,558 + -3.0
476,558 + +2.0
476,558 + +0.5
476,592 + ABZR87_RS18315 0.16 -2.1
476,592 + ABZR87_RS18315 0.16 -3.4
476,592 + ABZR87_RS18315 0.16 +0.2
476,593 - ABZR87_RS18315 0.16 +2.0
477,077 - ABZR87_RS18320 0.26 -1.3
477,077 - ABZR87_RS18320 0.26 +2.4

Or see this region's nucleotide sequence