Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS11845

Experiment: Sebacic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS11835 and ABZR87_RS11840 are separated by 176 nucleotidesABZR87_RS11840 and ABZR87_RS11845 are separated by 52 nucleotidesABZR87_RS11845 and ABZR87_RS11850 are separated by 3 nucleotidesABZR87_RS11850 and ABZR87_RS11855 are separated by 11 nucleotides ABZR87_RS11835: ABZR87_RS11835 - UMP kinase, at 2,514,132 to 2,514,842 _RS11835 ABZR87_RS11840: ABZR87_RS11840 - ribosome recycling factor, at 2,515,019 to 2,515,579 _RS11840 ABZR87_RS11845: ABZR87_RS11845 - polyprenyl diphosphate synthase, at 2,515,632 to 2,516,408 _RS11845 ABZR87_RS11850: ABZR87_RS11850 - phosphatidate cytidylyltransferase, at 2,516,412 to 2,517,230 _RS11850 ABZR87_RS11855: ABZR87_RS11855 - 1-deoxy-D-xylulose-5-phosphate reductoisomerase, at 2,517,242 to 2,518,432 _RS11855 Position (kb) 2515 2516 2517Strain fitness (log2 ratio) -3 -2 -1 0 1at 2514.877 kb on + strandat 2514.925 kb on + strandat 2514.925 kb on + strandat 2515.577 kb on + strandat 2515.577 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sebacic (C)
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2,514,877 + -0.3
2,514,925 + +0.6
2,514,925 + -3.3
2,515,577 + +0.1
2,515,577 + -1.9

Or see this region's nucleotide sequence