Experiment: BHIS with Ciprofloxacin Hydrochloride 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3770 and BT3771 are separated by 16 nucleotides BT3771 and BT3772 are separated by 9 nucleotides BT3772 and BT3773 are separated by 175 nucleotides
BT3770: BT3770 - transcriptional regulator (NCBI ptt file), at 4,891,205 to 4,891,795
BT3770
BT3771: BT3771 - 3-oxoacyl-[acyl-carrier protein] reductase (NCBI ptt file), at 4,891,812 to 4,892,558
BT3771
BT3772: BT3772 - ribosomal large subunit pseudouridine synthase D (NCBI ptt file), at 4,892,568 to 4,893,239
BT3772
BT3773: BT3773 - conserved hypothetical protein (NCBI ptt file), at 4,893,415 to 4,895,676
BT3773
Position (kb)
4892
4893
4894 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4891.591 kb on + strand, within BT3770 at 4891.670 kb on + strand, within BT3770 at 4891.780 kb on + strand at 4891.780 kb on + strand at 4891.780 kb on + strand at 4892.552 kb on + strand at 4892.552 kb on + strand at 4892.556 kb on + strand at 4892.567 kb on + strand at 4892.594 kb on + strand at 4892.596 kb on + strand at 4892.596 kb on + strand at 4892.596 kb on + strand at 4892.596 kb on + strand at 4892.680 kb on - strand, within BT3772 at 4892.680 kb on - strand, within BT3772 at 4892.695 kb on - strand, within BT3772 at 4892.889 kb on + strand, within BT3772 at 4892.890 kb on - strand, within BT3772 at 4893.094 kb on - strand, within BT3772 at 4893.371 kb on - strand at 4893.491 kb on - strand at 4893.517 kb on + strand at 4893.517 kb on + strand at 4893.530 kb on - strand at 4893.530 kb on - strand at 4893.558 kb on - strand at 4893.558 kb on - strand at 4893.562 kb on + strand at 4893.615 kb on + strand at 4893.647 kb on - strand, within BT3773 at 4893.647 kb on - strand, within BT3773 at 4893.687 kb on - strand, within BT3773 at 4893.702 kb on + strand, within BT3773 at 4893.703 kb on - strand, within BT3773 at 4893.727 kb on - strand, within BT3773 at 4893.774 kb on - strand, within BT3773 at 4893.774 kb on - strand, within BT3773 at 4893.774 kb on - strand, within BT3773 at 4893.816 kb on - strand, within BT3773 at 4893.833 kb on - strand, within BT3773 at 4893.853 kb on + strand, within BT3773 at 4893.854 kb on - strand, within BT3773 at 4893.854 kb on - strand, within BT3773 at 4893.858 kb on + strand, within BT3773 at 4893.880 kb on - strand, within BT3773 at 4893.880 kb on - strand, within BT3773 at 4893.880 kb on - strand, within BT3773 at 4893.906 kb on + strand, within BT3773 at 4893.907 kb on - strand, within BT3773 at 4893.907 kb on - strand, within BT3773 at 4893.934 kb on - strand, within BT3773 at 4893.934 kb on - strand, within BT3773 at 4893.935 kb on + strand, within BT3773 at 4893.983 kb on - strand, within BT3773 at 4893.983 kb on - strand, within BT3773 at 4893.983 kb on - strand, within BT3773 at 4893.996 kb on - strand, within BT3773 at 4894.004 kb on - strand, within BT3773 at 4894.005 kb on + strand, within BT3773 at 4894.006 kb on - strand, within BT3773 at 4894.057 kb on + strand at 4894.102 kb on + strand, within BT3773 at 4894.102 kb on + strand, within BT3773 at 4894.103 kb on - strand, within BT3773 at 4894.194 kb on + strand, within BT3773 at 4894.204 kb on - strand, within BT3773
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ciprofloxacin Hydrochloride 0.025 mM remove 4,891,591 + BT3770 0.65 -0.6 4,891,670 + BT3770 0.79 -1.9 4,891,780 + -2.3 4,891,780 + -4.0 4,891,780 + -0.1 4,892,552 + -1.2 4,892,552 + -1.2 4,892,556 + -0.6 4,892,567 + -1.9 4,892,594 + -1.2 4,892,596 + -1.8 4,892,596 + -0.5 4,892,596 + -0.3 4,892,596 + -0.9 4,892,680 - BT3772 0.17 +1.1 4,892,680 - BT3772 0.17 +0.0 4,892,695 - BT3772 0.19 +2.0 4,892,889 + BT3772 0.48 -0.6 4,892,890 - BT3772 0.48 +1.2 4,893,094 - BT3772 0.78 -0.6 4,893,371 - -0.6 4,893,491 - -1.7 4,893,517 + -0.6 4,893,517 + -1.7 4,893,530 - -1.2 4,893,530 - -1.0 4,893,558 - -2.6 4,893,558 - -0.6 4,893,562 + -2.6 4,893,615 + +1.1 4,893,647 - BT3773 0.10 -1.0 4,893,647 - BT3773 0.10 +2.4 4,893,687 - BT3773 0.12 -0.9 4,893,702 + BT3773 0.13 +0.7 4,893,703 - BT3773 0.13 -0.3 4,893,727 - BT3773 0.14 +0.4 4,893,774 - BT3773 0.16 -2.2 4,893,774 - BT3773 0.16 -1.2 4,893,774 - BT3773 0.16 +0.0 4,893,816 - BT3773 0.18 -1.4 4,893,833 - BT3773 0.18 -1.2 4,893,853 + BT3773 0.19 -1.3 4,893,854 - BT3773 0.19 -2.4 4,893,854 - BT3773 0.19 +0.4 4,893,858 + BT3773 0.20 -1.9 4,893,880 - BT3773 0.21 -0.2 4,893,880 - BT3773 0.21 +2.0 4,893,880 - BT3773 0.21 -0.8 4,893,906 + BT3773 0.22 -0.8 4,893,907 - BT3773 0.22 -0.6 4,893,907 - BT3773 0.22 -2.4 4,893,934 - BT3773 0.23 -1.2 4,893,934 - BT3773 0.23 -0.6 4,893,935 + BT3773 0.23 +1.0 4,893,983 - BT3773 0.25 +0.4 4,893,983 - BT3773 0.25 -2.4 4,893,983 - BT3773 0.25 +0.4 4,893,996 - BT3773 0.26 -1.6 4,894,004 - BT3773 0.26 -0.7 4,894,005 + BT3773 0.26 -0.6 4,894,006 - BT3773 0.26 -1.4 4,894,057 + -0.6 4,894,102 + BT3773 0.30 -2.2 4,894,102 + BT3773 0.30 -0.3 4,894,103 - BT3773 0.30 -1.2 4,894,194 + BT3773 0.34 -1.2 4,894,204 - BT3773 0.35 +1.0
Or see this region's nucleotide sequence