Experiment: BHIS with Ciprofloxacin Hydrochloride 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1783 and BT1784 are separated by 186 nucleotides BT1784 and BT1785 are separated by 83 nucleotides BT1785 and BT1786 overlap by 4 nucleotides
BT1783: BT1783 - cation/H+ antiporter (NCBI ptt file), at 2,228,777 to 2,231,002
BT1783
BT1784: BT1784 - hypothetical protein (NCBI ptt file), at 2,231,189 to 2,231,710
BT1784
BT1785: BT1785 - hypothetical protein (NCBI ptt file), at 2,231,794 to 2,232,999
BT1785
BT1786: BT1786 - hypothetical protein (NCBI ptt file), at 2,232,996 to 2,234,333
BT1786
Position (kb)
2231
2232
2233 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2230.798 kb on + strand at 2230.798 kb on + strand at 2230.799 kb on - strand at 2230.802 kb on + strand at 2230.803 kb on - strand at 2230.838 kb on - strand at 2230.871 kb on + strand at 2230.924 kb on + strand at 2230.964 kb on + strand at 2230.972 kb on + strand at 2230.994 kb on - strand at 2231.003 kb on - strand at 2231.035 kb on - strand at 2231.137 kb on + strand at 2231.138 kb on - strand at 2231.197 kb on - strand at 2231.388 kb on - strand, within BT1784 at 2231.393 kb on + strand, within BT1784 at 2231.398 kb on + strand, within BT1784 at 2231.399 kb on - strand, within BT1784 at 2231.399 kb on - strand, within BT1784 at 2231.400 kb on + strand, within BT1784 at 2231.545 kb on + strand, within BT1784 at 2231.554 kb on - strand, within BT1784 at 2231.647 kb on + strand, within BT1784 at 2231.648 kb on - strand, within BT1784 at 2231.669 kb on - strand at 2231.669 kb on - strand at 2231.682 kb on - strand at 2231.686 kb on - strand at 2231.704 kb on + strand at 2231.811 kb on + strand at 2231.813 kb on + strand at 2231.814 kb on - strand at 2231.898 kb on + strand at 2231.898 kb on + strand at 2231.998 kb on - strand, within BT1785 at 2232.000 kb on - strand, within BT1785 at 2232.046 kb on - strand, within BT1785 at 2232.064 kb on - strand, within BT1785 at 2232.064 kb on - strand, within BT1785 at 2232.107 kb on - strand, within BT1785 at 2232.108 kb on + strand, within BT1785 at 2232.180 kb on + strand, within BT1785 at 2232.185 kb on + strand, within BT1785 at 2232.186 kb on - strand, within BT1785 at 2232.357 kb on + strand, within BT1785 at 2232.357 kb on + strand, within BT1785 at 2232.358 kb on - strand, within BT1785 at 2232.358 kb on - strand, within BT1785 at 2232.360 kb on - strand, within BT1785 at 2232.363 kb on + strand, within BT1785 at 2232.365 kb on + strand, within BT1785 at 2232.366 kb on - strand, within BT1785 at 2232.408 kb on + strand, within BT1785 at 2232.409 kb on - strand, within BT1785 at 2232.451 kb on - strand, within BT1785 at 2232.464 kb on + strand, within BT1785 at 2232.519 kb on + strand, within BT1785 at 2232.567 kb on + strand, within BT1785 at 2232.568 kb on - strand, within BT1785 at 2232.616 kb on + strand, within BT1785 at 2232.616 kb on + strand, within BT1785 at 2232.617 kb on - strand, within BT1785 at 2232.617 kb on - strand, within BT1785 at 2232.617 kb on - strand, within BT1785 at 2232.816 kb on + strand, within BT1785 at 2232.817 kb on - strand, within BT1785 at 2232.819 kb on + strand, within BT1785 at 2232.819 kb on + strand at 2232.820 kb on - strand, within BT1785 at 2232.956 kb on - strand at 2232.973 kb on - strand at 2232.983 kb on - strand at 2232.983 kb on - strand at 2232.983 kb on - strand at 2233.004 kb on + strand at 2233.015 kb on + strand at 2233.018 kb on + strand at 2233.032 kb on + strand at 2233.033 kb on - strand at 2233.040 kb on + strand at 2233.094 kb on + strand at 2233.185 kb on - strand, within BT1786 at 2233.203 kb on - strand, within BT1786 at 2233.214 kb on - strand, within BT1786 at 2233.221 kb on - strand, within BT1786 at 2233.250 kb on + strand, within BT1786 at 2233.333 kb on + strand, within BT1786 at 2233.345 kb on + strand, within BT1786 at 2233.346 kb on - strand, within BT1786 at 2233.346 kb on - strand, within BT1786 at 2233.346 kb on - strand, within BT1786 at 2233.346 kb on - strand, within BT1786 at 2233.349 kb on + strand, within BT1786 at 2233.350 kb on - strand, within BT1786 at 2233.363 kb on + strand, within BT1786 at 2233.365 kb on + strand, within BT1786 at 2233.365 kb on + strand, within BT1786 at 2233.447 kb on + strand, within BT1786 at 2233.447 kb on + strand, within BT1786 at 2233.447 kb on + strand, within BT1786 at 2233.448 kb on - strand, within BT1786 at 2233.471 kb on + strand, within BT1786 at 2233.471 kb on + strand, within BT1786 at 2233.472 kb on - strand, within BT1786 at 2233.542 kb on - strand, within BT1786 at 2233.542 kb on - strand, within BT1786 at 2233.545 kb on - strand, within BT1786 at 2233.547 kb on + strand, within BT1786 at 2233.570 kb on + strand, within BT1786 at 2233.640 kb on - strand, within BT1786 at 2233.757 kb on + strand, within BT1786 at 2233.758 kb on - strand, within BT1786 at 2233.784 kb on + strand, within BT1786 at 2233.858 kb on - strand, within BT1786 at 2233.880 kb on + strand, within BT1786 at 2233.891 kb on + strand, within BT1786 at 2233.896 kb on - strand, within BT1786 at 2233.908 kb on - strand, within BT1786 at 2233.960 kb on - strand, within BT1786 at 2233.960 kb on - strand, within BT1786 at 2233.960 kb on - strand, within BT1786 at 2233.976 kb on - strand, within BT1786 at 2233.986 kb on - strand, within BT1786
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ciprofloxacin Hydrochloride 0.025 mM remove 2,230,798 + +0.5 2,230,798 + -0.5 2,230,799 - -0.2 2,230,802 + -0.5 2,230,803 - -0.5 2,230,838 - -2.1 2,230,871 + -0.8 2,230,924 + -1.1 2,230,964 + -1.4 2,230,972 + -0.6 2,230,994 - +0.5 2,231,003 - +0.5 2,231,035 - +0.5 2,231,137 + -1.3 2,231,138 - +1.8 2,231,197 - -1.8 2,231,388 - BT1784 0.38 -1.3 2,231,393 + BT1784 0.39 +0.5 2,231,398 + BT1784 0.40 -0.9 2,231,399 - BT1784 0.40 -2.8 2,231,399 - BT1784 0.40 -0.4 2,231,400 + BT1784 0.40 -1.4 2,231,545 + BT1784 0.68 +1.1 2,231,554 - BT1784 0.70 -0.1 2,231,647 + BT1784 0.88 -1.4 2,231,648 - BT1784 0.88 -0.5 2,231,669 - +0.3 2,231,669 - +0.5 2,231,682 - -1.5 2,231,686 - -1.5 2,231,704 + -0.1 2,231,811 + -1.2 2,231,813 + -1.8 2,231,814 - +0.3 2,231,898 + -0.8 2,231,898 + +0.5 2,231,998 - BT1785 0.17 +1.1 2,232,000 - BT1785 0.17 -0.7 2,232,046 - BT1785 0.21 -0.1 2,232,064 - BT1785 0.22 -0.2 2,232,064 - BT1785 0.22 +0.5 2,232,107 - BT1785 0.26 +0.5 2,232,108 + BT1785 0.26 +0.5 2,232,180 + BT1785 0.32 -0.3 2,232,185 + BT1785 0.32 -0.8 2,232,186 - BT1785 0.33 +0.5 2,232,357 + BT1785 0.47 -3.1 2,232,357 + BT1785 0.47 -1.8 2,232,358 - BT1785 0.47 -1.1 2,232,358 - BT1785 0.47 -1.0 2,232,360 - BT1785 0.47 -1.5 2,232,363 + BT1785 0.47 -1.1 2,232,365 + BT1785 0.47 +0.5 2,232,366 - BT1785 0.47 +0.5 2,232,408 + BT1785 0.51 -1.8 2,232,409 - BT1785 0.51 -0.5 2,232,451 - BT1785 0.54 +0.5 2,232,464 + BT1785 0.56 +0.6 2,232,519 + BT1785 0.60 -0.4 2,232,567 + BT1785 0.64 +0.5 2,232,568 - BT1785 0.64 -0.5 2,232,616 + BT1785 0.68 +0.5 2,232,616 + BT1785 0.68 -2.1 2,232,617 - BT1785 0.68 +0.7 2,232,617 - BT1785 0.68 +0.5 2,232,617 - BT1785 0.68 -1.5 2,232,816 + BT1785 0.85 -0.5 2,232,817 - BT1785 0.85 +0.2 2,232,819 + BT1785 0.85 -0.5 2,232,819 + +0.5 2,232,820 - BT1785 0.85 -0.5 2,232,956 - -1.1 2,232,973 - -0.1 2,232,983 - +0.2 2,232,983 - -0.1 2,232,983 - -0.5 2,233,004 + -1.1 2,233,015 + -2.8 2,233,018 + -0.8 2,233,032 + -0.5 2,233,033 - +0.5 2,233,040 + -1.0 2,233,094 + +0.5 2,233,185 - BT1786 0.14 -0.5 2,233,203 - BT1786 0.15 +0.5 2,233,214 - BT1786 0.16 -1.1 2,233,221 - BT1786 0.17 -0.7 2,233,250 + BT1786 0.19 -1.1 2,233,333 + BT1786 0.25 -0.5 2,233,345 + BT1786 0.26 -0.1 2,233,346 - BT1786 0.26 -1.5 2,233,346 - BT1786 0.26 -0.5 2,233,346 - BT1786 0.26 -1.5 2,233,346 - BT1786 0.26 -1.7 2,233,349 + BT1786 0.26 +1.1 2,233,350 - BT1786 0.26 -0.3 2,233,363 + BT1786 0.27 +0.3 2,233,365 + BT1786 0.28 -1.8 2,233,365 + BT1786 0.28 -2.3 2,233,447 + BT1786 0.34 -1.5 2,233,447 + BT1786 0.34 -2.1 2,233,447 + BT1786 0.34 +0.3 2,233,448 - BT1786 0.34 -0.2 2,233,471 + BT1786 0.36 -1.1 2,233,471 + BT1786 0.36 +0.3 2,233,472 - BT1786 0.36 -1.5 2,233,542 - BT1786 0.41 -1.5 2,233,542 - BT1786 0.41 -0.2 2,233,545 - BT1786 0.41 +0.1 2,233,547 + BT1786 0.41 -1.8 2,233,570 + BT1786 0.43 -0.1 2,233,640 - BT1786 0.48 -2.1 2,233,757 + BT1786 0.57 -0.1 2,233,758 - BT1786 0.57 -2.3 2,233,784 + BT1786 0.59 +0.1 2,233,858 - BT1786 0.64 +0.5 2,233,880 + BT1786 0.66 -0.8 2,233,891 + BT1786 0.67 -0.9 2,233,896 - BT1786 0.67 -0.5 2,233,908 - BT1786 0.68 -2.1 2,233,960 - BT1786 0.72 -0.5 2,233,960 - BT1786 0.72 -1.3 2,233,960 - BT1786 0.72 -1.1 2,233,976 - BT1786 0.73 -0.8 2,233,986 - BT1786 0.74 -0.5
Or see this region's nucleotide sequence