Experiment: BHIS with Ciprofloxacin Hydrochloride 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0455 and BT0456 overlap by 4 nucleotides BT0456 and BT0457 are separated by 11 nucleotides
BT0455: BT0455 - sialidase (Neuraminidase) (NCBI ptt file), at 557,455 to 559,089
BT0455
BT0456: BT0456 - beta-hexosaminidase precursor (NCBI ptt file), at 559,086 to 561,098
BT0456
BT0457: BT0457 - sialic acid-specific 9-O-acetylesterase (NCBI ptt file), at 561,110 to 563,188
BT0457
Position (kb)
559
560
561
562 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 558.112 kb on + strand, within BT0455 at 558.113 kb on - strand, within BT0455 at 558.113 kb on - strand, within BT0455 at 558.124 kb on + strand, within BT0455 at 558.127 kb on - strand, within BT0455 at 558.129 kb on + strand, within BT0455 at 558.130 kb on - strand, within BT0455 at 558.130 kb on - strand, within BT0455 at 558.152 kb on - strand, within BT0455 at 558.156 kb on - strand, within BT0455 at 558.276 kb on + strand, within BT0455 at 558.277 kb on - strand, within BT0455 at 558.426 kb on + strand, within BT0455 at 558.451 kb on - strand, within BT0455 at 558.595 kb on - strand, within BT0455 at 558.595 kb on - strand, within BT0455 at 558.659 kb on + strand, within BT0455 at 558.798 kb on - strand, within BT0455 at 558.802 kb on - strand, within BT0455 at 558.835 kb on - strand, within BT0455 at 558.847 kb on - strand, within BT0455 at 558.852 kb on + strand, within BT0455 at 558.898 kb on - strand, within BT0455 at 558.907 kb on - strand, within BT0455 at 558.985 kb on - strand at 559.138 kb on - strand at 559.139 kb on + strand at 559.142 kb on - strand at 559.142 kb on - strand at 559.284 kb on - strand at 559.311 kb on - strand, within BT0456 at 559.319 kb on - strand, within BT0456 at 559.345 kb on + strand, within BT0456 at 559.345 kb on + strand, within BT0456 at 559.411 kb on + strand, within BT0456 at 559.447 kb on + strand, within BT0456 at 559.448 kb on - strand, within BT0456 at 559.497 kb on - strand, within BT0456 at 559.499 kb on + strand, within BT0456 at 559.547 kb on - strand, within BT0456 at 559.558 kb on - strand, within BT0456 at 559.723 kb on - strand, within BT0456 at 559.761 kb on - strand, within BT0456 at 559.923 kb on - strand, within BT0456 at 559.981 kb on - strand, within BT0456 at 560.032 kb on - strand, within BT0456 at 560.153 kb on - strand, within BT0456 at 560.153 kb on - strand, within BT0456 at 560.169 kb on - strand, within BT0456 at 560.261 kb on + strand, within BT0456 at 560.338 kb on - strand, within BT0456 at 560.423 kb on - strand, within BT0456 at 560.464 kb on - strand, within BT0456 at 560.566 kb on - strand, within BT0456 at 560.566 kb on - strand, within BT0456 at 560.567 kb on + strand, within BT0456 at 560.568 kb on - strand, within BT0456 at 560.571 kb on + strand, within BT0456 at 560.616 kb on - strand, within BT0456 at 560.633 kb on - strand, within BT0456 at 560.689 kb on - strand, within BT0456 at 560.689 kb on - strand, within BT0456 at 560.695 kb on - strand, within BT0456 at 560.700 kb on + strand, within BT0456 at 560.862 kb on - strand, within BT0456 at 560.943 kb on - strand at 561.002 kb on - strand at 561.031 kb on + strand at 561.108 kb on - strand at 561.126 kb on + strand at 561.152 kb on - strand at 561.215 kb on + strand at 561.242 kb on - strand at 561.347 kb on + strand, within BT0457 at 561.348 kb on - strand, within BT0457 at 561.394 kb on + strand at 561.424 kb on + strand, within BT0457 at 561.425 kb on - strand at 561.539 kb on - strand, within BT0457 at 561.539 kb on - strand, within BT0457 at 561.539 kb on - strand, within BT0457 at 561.539 kb on - strand, within BT0457 at 561.700 kb on - strand, within BT0457 at 561.724 kb on + strand, within BT0457 at 561.744 kb on - strand, within BT0457 at 561.793 kb on + strand, within BT0457 at 561.871 kb on - strand, within BT0457 at 561.961 kb on + strand, within BT0457
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ciprofloxacin Hydrochloride 0.025 mM remove 558,112 + BT0455 0.40 +0.1 558,113 - BT0455 0.40 -2.2 558,113 - BT0455 0.40 -0.7 558,124 + BT0455 0.41 -2.2 558,127 - BT0455 0.41 -0.8 558,129 + BT0455 0.41 -1.4 558,130 - BT0455 0.41 -0.4 558,130 - BT0455 0.41 -0.9 558,152 - BT0455 0.43 +1.6 558,156 - BT0455 0.43 -1.6 558,276 + BT0455 0.50 -0.5 558,277 - BT0455 0.50 +0.6 558,426 + BT0455 0.59 +0.1 558,451 - BT0455 0.61 -0.4 558,595 - BT0455 0.70 -0.4 558,595 - BT0455 0.70 -0.9 558,659 + BT0455 0.74 -1.7 558,798 - BT0455 0.82 -1.5 558,802 - BT0455 0.82 -0.5 558,835 - BT0455 0.84 -2.4 558,847 - BT0455 0.85 -0.4 558,852 + BT0455 0.85 +2.6 558,898 - BT0455 0.88 -0.6 558,907 - BT0455 0.89 -0.4 558,985 - -0.4 559,138 - +0.6 559,139 + -0.4 559,142 - +0.3 559,142 - +0.6 559,284 - -0.3 559,311 - BT0456 0.11 +0.7 559,319 - BT0456 0.12 -0.3 559,345 + BT0456 0.13 -0.3 559,345 + BT0456 0.13 -0.3 559,411 + BT0456 0.16 +1.7 559,447 + BT0456 0.18 -1.7 559,448 - BT0456 0.18 +0.7 559,497 - BT0456 0.20 +1.7 559,499 + BT0456 0.21 -1.3 559,547 - BT0456 0.23 -1.9 559,558 - BT0456 0.23 +0.7 559,723 - BT0456 0.32 +1.2 559,761 - BT0456 0.34 +2.1 559,923 - BT0456 0.42 -0.3 559,981 - BT0456 0.44 +0.7 560,032 - BT0456 0.47 +0.2 560,153 - BT0456 0.53 +0.7 560,153 - BT0456 0.53 +0.7 560,169 - BT0456 0.54 -0.3 560,261 + BT0456 0.58 -2.3 560,338 - BT0456 0.62 +0.7 560,423 - BT0456 0.66 +0.7 560,464 - BT0456 0.68 -0.5 560,566 - BT0456 0.74 -1.3 560,566 - BT0456 0.74 -0.3 560,567 + BT0456 0.74 +0.7 560,568 - BT0456 0.74 -0.3 560,571 + BT0456 0.74 +0.7 560,616 - BT0456 0.76 -0.3 560,633 - BT0456 0.77 -0.5 560,689 - BT0456 0.80 -1.3 560,689 - BT0456 0.80 -1.3 560,695 - BT0456 0.80 -1.3 560,700 + BT0456 0.80 +2.2 560,862 - BT0456 0.88 +0.7 560,943 - -1.2 561,002 - -0.9 561,031 + -0.9 561,108 - -0.8 561,126 + +0.7 561,152 - -0.9 561,215 + +0.7 561,242 - -1.7 561,347 + BT0457 0.11 +0.7 561,348 - BT0457 0.11 -0.3 561,394 + +0.7 561,424 + BT0457 0.15 +0.7 561,425 - +0.7 561,539 - BT0457 0.21 +0.7 561,539 - BT0457 0.21 -0.9 561,539 - BT0457 0.21 -2.0 561,539 - BT0457 0.21 -1.2 561,700 - BT0457 0.28 -2.3 561,724 + BT0457 0.30 -0.7 561,744 - BT0457 0.30 -0.1 561,793 + BT0457 0.33 -0.7 561,871 - BT0457 0.37 -1.3 561,961 + BT0457 0.41 -2.8
Or see this region's nucleotide sequence