Experiment: BG11 with Chlorite 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tgt and psbK are separated by 53 nucleotides psbK and Synpcc7942_0457 are separated by 215 nucleotides Synpcc7942_0457 and fghA are separated by 35 nucleotides fghA and Synpcc7942_0459 are separated by 5 nucleotides
Synpcc7942_0455: tgt - queuine tRNA-ribosyltransferase, at 444,001 to 445,155
tgt
Synpcc7942_0456: psbK - photosystem II reaction center protein PsbK precursor, at 445,209 to 445,346
psbK
Synpcc7942_0457: Synpcc7942_0457 - hypothetical protein, at 445,562 to 446,179
_0457
Synpcc7942_0458: fghA - Carboxylesterase, at 446,215 to 447,051
fghA
Synpcc7942_0459: Synpcc7942_0459 - glutathione-dependent formaldehyde dehydrogenase, at 447,057 to 448,166
_0459
Position (kb) 445
446
447 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 444.576 kb on - strand, within tgt at 444.595 kb on + strand, within tgt at 444.595 kb on + strand, within tgt at 444.595 kb on + strand, within tgt at 444.595 kb on + strand, within tgt at 444.603 kb on - strand, within tgt at 444.603 kb on - strand, within tgt at 444.603 kb on - strand, within tgt at 444.603 kb on - strand, within tgt at 444.738 kb on + strand, within tgt at 444.749 kb on + strand, within tgt at 444.749 kb on + strand, within tgt at 444.749 kb on + strand, within tgt at 444.757 kb on - strand, within tgt at 444.773 kb on + strand, within tgt at 444.796 kb on + strand, within tgt at 444.858 kb on + strand, within tgt at 444.858 kb on + strand, within tgt at 444.871 kb on - strand, within tgt at 444.902 kb on + strand, within tgt at 444.948 kb on + strand, within tgt at 445.014 kb on + strand, within tgt at 445.022 kb on - strand, within tgt at 445.092 kb on - strand at 445.116 kb on + strand at 445.129 kb on - strand at 445.129 kb on - strand at 445.148 kb on - strand at 445.208 kb on + strand at 445.218 kb on + strand at 445.293 kb on - strand, within psbK at 445.349 kb on - strand at 445.356 kb on - strand at 445.364 kb on - strand at 445.395 kb on + strand at 445.446 kb on - strand at 445.573 kb on - strand at 445.583 kb on + strand at 445.593 kb on + strand at 445.601 kb on - strand at 445.641 kb on + strand, within Synpcc7942_0457 at 445.670 kb on + strand, within Synpcc7942_0457 at 445.670 kb on + strand at 445.670 kb on + strand, within Synpcc7942_0457 at 445.670 kb on + strand, within Synpcc7942_0457 at 445.675 kb on + strand at 445.678 kb on - strand, within Synpcc7942_0457 at 445.678 kb on - strand, within Synpcc7942_0457 at 445.678 kb on - strand, within Synpcc7942_0457 at 445.678 kb on - strand, within Synpcc7942_0457 at 445.681 kb on + strand, within Synpcc7942_0457 at 445.686 kb on + strand, within Synpcc7942_0457 at 445.689 kb on - strand, within Synpcc7942_0457 at 445.691 kb on - strand, within Synpcc7942_0457 at 445.755 kb on + strand, within Synpcc7942_0457 at 445.763 kb on - strand, within Synpcc7942_0457 at 445.771 kb on + strand, within Synpcc7942_0457 at 445.783 kb on + strand, within Synpcc7942_0457 at 445.790 kb on + strand, within Synpcc7942_0457 at 445.798 kb on - strand, within Synpcc7942_0457 at 445.799 kb on - strand, within Synpcc7942_0457 at 445.806 kb on + strand, within Synpcc7942_0457 at 445.814 kb on - strand, within Synpcc7942_0457 at 445.832 kb on + strand, within Synpcc7942_0457 at 445.874 kb on - strand, within Synpcc7942_0457 at 445.897 kb on + strand, within Synpcc7942_0457 at 445.900 kb on - strand, within Synpcc7942_0457 at 445.933 kb on + strand, within Synpcc7942_0457 at 445.933 kb on + strand, within Synpcc7942_0457 at 445.933 kb on + strand, within Synpcc7942_0457 at 445.938 kb on + strand, within Synpcc7942_0457 at 445.941 kb on - strand, within Synpcc7942_0457 at 445.951 kb on - strand, within Synpcc7942_0457 at 445.988 kb on - strand, within Synpcc7942_0457 at 446.071 kb on + strand, within Synpcc7942_0457 at 446.120 kb on + strand at 446.169 kb on + strand at 446.169 kb on + strand at 446.185 kb on + strand at 446.200 kb on - strand at 446.234 kb on + strand at 446.242 kb on - strand at 446.287 kb on + strand at 446.318 kb on - strand, within fghA at 446.324 kb on - strand, within fghA at 446.337 kb on - strand, within fghA at 446.343 kb on + strand, within fghA at 446.453 kb on + strand, within fghA at 446.468 kb on - strand, within fghA at 446.477 kb on + strand, within fghA at 446.499 kb on - strand, within fghA at 446.507 kb on + strand, within fghA at 446.515 kb on - strand, within fghA at 446.533 kb on + strand, within fghA at 446.545 kb on + strand, within fghA at 446.553 kb on - strand, within fghA at 446.570 kb on + strand, within fghA at 446.578 kb on - strand, within fghA at 446.580 kb on + strand, within fghA at 446.588 kb on - strand, within fghA at 446.595 kb on - strand, within fghA at 446.665 kb on + strand, within fghA at 446.700 kb on + strand, within fghA at 446.708 kb on - strand, within fghA at 446.710 kb on - strand, within fghA at 446.741 kb on + strand, within fghA at 446.749 kb on - strand, within fghA at 446.782 kb on + strand, within fghA at 446.888 kb on + strand, within fghA at 446.896 kb on - strand, within fghA at 446.901 kb on - strand, within fghA at 446.901 kb on - strand, within fghA at 446.909 kb on + strand, within fghA at 446.917 kb on - strand, within fghA at 446.930 kb on - strand, within fghA at 446.930 kb on - strand, within fghA at 446.932 kb on - strand, within fghA at 446.947 kb on - strand, within fghA at 446.960 kb on - strand, within fghA at 446.965 kb on - strand, within fghA at 446.977 kb on + strand at 446.979 kb on + strand at 446.984 kb on + strand at 446.985 kb on - strand at 446.991 kb on + strand at 446.999 kb on - strand at 447.007 kb on - strand at 447.035 kb on + strand at 447.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Chlorite 0.3125 mM remove 444,576 - tgt Synpcc7942_0455 0.50 -1.3 444,595 + tgt Synpcc7942_0455 0.51 -2.0 444,595 + tgt Synpcc7942_0455 0.51 +1.5 444,595 + tgt Synpcc7942_0455 0.51 -1.8 444,595 + tgt Synpcc7942_0455 0.51 -1.3 444,603 - tgt Synpcc7942_0455 0.52 +1.5 444,603 - tgt Synpcc7942_0455 0.52 +0.1 444,603 - tgt Synpcc7942_0455 0.52 +1.7 444,603 - tgt Synpcc7942_0455 0.52 -0.2 444,738 + tgt Synpcc7942_0455 0.64 -1.7 444,749 + tgt Synpcc7942_0455 0.65 -2.0 444,749 + tgt Synpcc7942_0455 0.65 -0.9 444,749 + tgt Synpcc7942_0455 0.65 -0.1 444,757 - tgt Synpcc7942_0455 0.65 +0.3 444,773 + tgt Synpcc7942_0455 0.67 -1.2 444,796 + tgt Synpcc7942_0455 0.69 -1.0 444,858 + tgt Synpcc7942_0455 0.74 -1.0 444,858 + tgt Synpcc7942_0455 0.74 -1.7 444,871 - tgt Synpcc7942_0455 0.75 -3.5 444,902 + tgt Synpcc7942_0455 0.78 -4.1 444,948 + tgt Synpcc7942_0455 0.82 -2.6 445,014 + tgt Synpcc7942_0455 0.88 -1.9 445,022 - tgt Synpcc7942_0455 0.88 -1.2 445,092 - -0.7 445,116 + +0.1 445,129 - +0.1 445,129 - +1.0 445,148 - +0.9 445,208 + -0.6 445,218 + +1.6 445,293 - psbK Synpcc7942_0456 0.61 -0.0 445,349 - +0.4 445,356 - -1.3 445,364 - +0.0 445,395 + +0.5 445,446 - +1.0 445,573 - +0.5 445,583 + +0.2 445,593 + -0.1 445,601 - -0.9 445,641 + Synpcc7942_0457 0.13 +0.6 445,670 + Synpcc7942_0457 0.17 -1.5 445,670 + +0.5 445,670 + Synpcc7942_0457 0.17 -0.3 445,670 + Synpcc7942_0457 0.17 -0.2 445,675 + +1.1 445,678 - Synpcc7942_0457 0.19 -0.3 445,678 - Synpcc7942_0457 0.19 +1.4 445,678 - Synpcc7942_0457 0.19 +1.1 445,678 - Synpcc7942_0457 0.19 +1.4 445,681 + Synpcc7942_0457 0.19 -0.0 445,686 + Synpcc7942_0457 0.20 -0.4 445,689 - Synpcc7942_0457 0.21 -1.9 445,691 - Synpcc7942_0457 0.21 +2.0 445,755 + Synpcc7942_0457 0.31 -1.0 445,763 - Synpcc7942_0457 0.33 -2.0 445,771 + Synpcc7942_0457 0.34 +0.4 445,783 + Synpcc7942_0457 0.36 +1.1 445,790 + Synpcc7942_0457 0.37 -0.6 445,798 - Synpcc7942_0457 0.38 +0.4 445,799 - Synpcc7942_0457 0.38 +0.5 445,806 + Synpcc7942_0457 0.39 +1.5 445,814 - Synpcc7942_0457 0.41 +0.3 445,832 + Synpcc7942_0457 0.44 -2.6 445,874 - Synpcc7942_0457 0.50 -1.0 445,897 + Synpcc7942_0457 0.54 -0.0 445,900 - Synpcc7942_0457 0.55 +0.4 445,933 + Synpcc7942_0457 0.60 +0.4 445,933 + Synpcc7942_0457 0.60 -0.0 445,933 + Synpcc7942_0457 0.60 +1.5 445,938 + Synpcc7942_0457 0.61 -1.3 445,941 - Synpcc7942_0457 0.61 -2.2 445,951 - Synpcc7942_0457 0.63 +2.1 445,988 - Synpcc7942_0457 0.69 +1.4 446,071 + Synpcc7942_0457 0.82 +1.7 446,120 + -0.4 446,169 + -0.0 446,169 + -0.7 446,185 + +2.5 446,200 - +0.2 446,234 + -0.3 446,242 - -0.8 446,287 + -0.3 446,318 - fghA Synpcc7942_0458 0.12 -0.7 446,324 - fghA Synpcc7942_0458 0.13 -2.2 446,337 - fghA Synpcc7942_0458 0.15 +1.3 446,343 + fghA Synpcc7942_0458 0.15 +1.3 446,453 + fghA Synpcc7942_0458 0.28 -1.5 446,468 - fghA Synpcc7942_0458 0.30 +0.4 446,477 + fghA Synpcc7942_0458 0.31 +0.1 446,499 - fghA Synpcc7942_0458 0.34 +0.9 446,507 + fghA Synpcc7942_0458 0.35 -0.3 446,515 - fghA Synpcc7942_0458 0.36 -0.5 446,533 + fghA Synpcc7942_0458 0.38 -0.9 446,545 + fghA Synpcc7942_0458 0.39 +0.5 446,553 - fghA Synpcc7942_0458 0.40 -0.2 446,570 + fghA Synpcc7942_0458 0.42 -0.9 446,578 - fghA Synpcc7942_0458 0.43 -0.1 446,580 + fghA Synpcc7942_0458 0.44 +0.2 446,588 - fghA Synpcc7942_0458 0.45 +0.0 446,595 - fghA Synpcc7942_0458 0.45 -1.0 446,665 + fghA Synpcc7942_0458 0.54 -2.5 446,700 + fghA Synpcc7942_0458 0.58 +0.5 446,708 - fghA Synpcc7942_0458 0.59 -0.6 446,710 - fghA Synpcc7942_0458 0.59 +0.5 446,741 + fghA Synpcc7942_0458 0.63 -2.0 446,749 - fghA Synpcc7942_0458 0.64 -1.2 446,782 + fghA Synpcc7942_0458 0.68 -0.3 446,888 + fghA Synpcc7942_0458 0.80 -0.9 446,896 - fghA Synpcc7942_0458 0.81 -0.6 446,901 - fghA Synpcc7942_0458 0.82 -0.3 446,901 - fghA Synpcc7942_0458 0.82 +0.0 446,909 + fghA Synpcc7942_0458 0.83 +0.8 446,917 - fghA Synpcc7942_0458 0.84 +1.5 446,930 - fghA Synpcc7942_0458 0.85 -1.1 446,930 - fghA Synpcc7942_0458 0.85 -0.2 446,932 - fghA Synpcc7942_0458 0.86 +0.3 446,947 - fghA Synpcc7942_0458 0.87 -1.2 446,960 - fghA Synpcc7942_0458 0.89 +0.0 446,965 - fghA Synpcc7942_0458 0.90 +1.3 446,977 + -1.1 446,979 + +0.8 446,984 + +0.1 446,985 - +0.1 446,991 + -0.7 446,999 - +0.5 447,007 - -0.0 447,035 + -0.7 447,064 - +1.5
Or see this region's nucleotide sequence