Experiment: MoLS4 with L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nifH and nifV are separated by 757 nucleotides nifV and DORF123 are separated by 366 nucleotides
DVUA0015: nifH - nitrogenase iron protein (TIGR), at 18,877 to 19,710
nifH
DVUA0016: nifV - homocitrate synthase (TIGR), at 20,468 to 21,622
nifV
DORF123: DORF123 - SEED: FIG00603348: hypothetical protein, at 21,989 to 22,207
DORF123
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-2
-1
0
1 at 19.540 kb on - strand, within nifH at 19.600 kb on + strand, within nifH at 19.600 kb on + strand, within nifH at 19.641 kb on + strand at 19.652 kb on - strand at 19.732 kb on - strand at 19.780 kb on + strand at 19.801 kb on - strand at 19.838 kb on + strand at 19.927 kb on - strand at 19.938 kb on - strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.950 kb on - strand at 19.950 kb on - strand at 19.950 kb on - strand at 19.972 kb on - strand at 19.979 kb on + strand at 19.987 kb on - strand at 20.024 kb on + strand at 20.032 kb on - strand at 20.089 kb on - strand at 20.132 kb on - strand at 20.280 kb on - strand at 20.373 kb on + strand at 20.401 kb on + strand at 20.406 kb on + strand at 20.409 kb on - strand at 20.409 kb on - strand at 20.411 kb on + strand at 20.461 kb on + strand at 20.519 kb on + strand at 20.544 kb on + strand at 20.569 kb on + strand at 20.571 kb on - strand at 20.577 kb on - strand at 20.577 kb on - strand at 20.671 kb on - strand, within nifV at 20.699 kb on - strand, within nifV at 20.758 kb on + strand, within nifV at 20.761 kb on - strand, within nifV at 20.843 kb on + strand, within nifV at 20.876 kb on + strand, within nifV at 20.954 kb on - strand, within nifV at 20.976 kb on - strand, within nifV at 21.026 kb on - strand, within nifV at 21.068 kb on + strand, within nifV at 21.075 kb on + strand, within nifV at 21.093 kb on - strand, within nifV at 21.211 kb on - strand, within nifV at 21.211 kb on - strand, within nifV at 21.211 kb on - strand, within nifV at 21.220 kb on + strand, within nifV at 21.331 kb on - strand, within nifV at 21.333 kb on + strand, within nifV at 21.380 kb on - strand, within nifV at 21.380 kb on - strand, within nifV at 21.418 kb on + strand, within nifV at 21.441 kb on - strand, within nifV at 21.441 kb on - strand, within nifV at 21.487 kb on - strand, within nifV at 21.504 kb on + strand, within nifV at 21.509 kb on + strand at 21.512 kb on - strand at 21.518 kb on - strand at 21.530 kb on + strand at 21.533 kb on - strand at 21.550 kb on - strand at 21.584 kb on + strand at 21.592 kb on - strand at 21.612 kb on - strand at 21.619 kb on - strand at 21.644 kb on - strand at 21.655 kb on + strand at 21.708 kb on - strand at 21.708 kb on - strand at 21.708 kb on - strand at 21.708 kb on - strand at 21.725 kb on - strand at 21.751 kb on + strand at 21.759 kb on - strand at 21.780 kb on - strand at 21.859 kb on + strand at 21.922 kb on - strand at 21.948 kb on + strand at 22.013 kb on - strand, within DORF123 at 22.013 kb on - strand, within DORF123 at 22.024 kb on - strand, within DORF123 at 22.024 kb on - strand, within DORF123 at 22.077 kb on - strand, within DORF123 at 22.077 kb on - strand, within DORF123 at 22.077 kb on - strand, within DORF123 at 22.079 kb on - strand, within DORF123 at 22.084 kb on + strand, within DORF123 at 22.084 kb on + strand, within DORF123 at 22.092 kb on - strand, within DORF123 at 22.101 kb on + strand, within DORF123 at 22.220 kb on - strand at 22.226 kb on + strand at 22.226 kb on - strand at 22.233 kb on - strand at 22.234 kb on - strand at 22.363 kb on + strand at 22.386 kb on - strand at 22.397 kb on + strand at 22.397 kb on - strand at 22.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Phenylalanine (N) remove 19,540 - nifH DVUA0015 0.79 -0.7 19,600 + nifH DVUA0015 0.87 -0.6 19,600 + nifH DVUA0015 0.87 -1.7 19,641 + -1.2 19,652 - -0.4 19,732 - -0.0 19,780 + -1.0 19,801 - -0.8 19,838 + -0.9 19,927 - -0.8 19,938 - -0.7 19,942 + -0.7 19,942 + +0.5 19,942 + -0.7 19,942 + -0.9 19,942 + -0.2 19,942 + -0.6 19,942 + +0.1 19,950 - -0.4 19,950 - -0.8 19,950 - -1.0 19,972 - -0.8 19,979 + -0.7 19,987 - -0.8 20,024 + -0.4 20,032 - -0.3 20,089 - -0.2 20,132 - -1.3 20,280 - -0.0 20,373 + +0.1 20,401 + -0.2 20,406 + +0.6 20,409 - -0.3 20,409 - +0.1 20,411 + -0.4 20,461 + -0.2 20,519 + -0.4 20,544 + -0.7 20,569 + -0.8 20,571 - -0.6 20,577 - +0.7 20,577 - -1.3 20,671 - nifV DVUA0016 0.18 -0.2 20,699 - nifV DVUA0016 0.20 -0.8 20,758 + nifV DVUA0016 0.25 -0.6 20,761 - nifV DVUA0016 0.25 -0.9 20,843 + nifV DVUA0016 0.32 -1.7 20,876 + nifV DVUA0016 0.35 -1.5 20,954 - nifV DVUA0016 0.42 -0.6 20,976 - nifV DVUA0016 0.44 -1.0 21,026 - nifV DVUA0016 0.48 -0.6 21,068 + nifV DVUA0016 0.52 -0.7 21,075 + nifV DVUA0016 0.53 -0.8 21,093 - nifV DVUA0016 0.54 -0.8 21,211 - nifV DVUA0016 0.64 -1.4 21,211 - nifV DVUA0016 0.64 -0.7 21,211 - nifV DVUA0016 0.64 -0.4 21,220 + nifV DVUA0016 0.65 -0.5 21,331 - nifV DVUA0016 0.75 -0.7 21,333 + nifV DVUA0016 0.75 -0.9 21,380 - nifV DVUA0016 0.79 -0.7 21,380 - nifV DVUA0016 0.79 -1.3 21,418 + nifV DVUA0016 0.82 -1.3 21,441 - nifV DVUA0016 0.84 +1.3 21,441 - nifV DVUA0016 0.84 -1.1 21,487 - nifV DVUA0016 0.88 -0.8 21,504 + nifV DVUA0016 0.90 +0.9 21,509 + -1.1 21,512 - -1.1 21,518 - -0.6 21,530 + -0.3 21,533 - -0.2 21,550 - -1.5 21,584 + -0.9 21,592 - +0.8 21,612 - -0.4 21,619 - +0.4 21,644 - -0.4 21,655 + -0.3 21,708 - +0.2 21,708 - +0.1 21,708 - +0.1 21,708 - +0.2 21,725 - +0.1 21,751 + -0.2 21,759 - -0.2 21,780 - +0.2 21,859 + -0.1 21,922 - +0.5 21,948 + -0.2 22,013 - DORF123 0.11 -0.3 22,013 - DORF123 0.11 -0.4 22,024 - DORF123 0.16 -0.0 22,024 - DORF123 0.16 -0.2 22,077 - DORF123 0.40 -0.6 22,077 - DORF123 0.40 -0.0 22,077 - DORF123 0.40 -0.4 22,079 - DORF123 0.41 -0.1 22,084 + DORF123 0.43 +0.7 22,084 + DORF123 0.43 -1.8 22,092 - DORF123 0.47 +0.4 22,101 + DORF123 0.51 -1.5 22,220 - -0.5 22,226 + -0.3 22,226 - -0.1 22,233 - +0.0 22,234 - +0.0 22,363 + +0.7 22,386 - +0.3 22,397 + +0.7 22,397 - +0.2 22,405 - -0.1
Or see this region's nucleotide sequence