Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4414

Experiment: BHIS with 5-Fluorouracil 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4412 and BT4413 are separated by 83 nucleotidesBT4413 and BT4414 are separated by 220 nucleotidesBT4414 and BT4415 are separated by 14 nucleotides BT4412: BT4412 - conserved hypothetical protein (NCBI ptt file), at 5,814,323 to 5,817,040 BT4412 BT4413: BT4413 - hypothetical protein (NCBI ptt file), at 5,817,124 to 5,817,561 BT4413 BT4414: BT4414 - hypothetical protein (NCBI ptt file), at 5,817,782 to 5,818,333 BT4414 BT4415: BT4415 - putative decarboxylase beta subunit (NCBI ptt file), at 5,818,348 to 5,819,562 BT4415 Position (kb) 5817 5818 5819Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5816.815 kb on + strandat 5816.944 kb on + strandat 5816.992 kb on + strandat 5816.992 kb on + strandat 5816.993 kb on - strandat 5817.025 kb on - strandat 5817.028 kb on - strandat 5817.036 kb on + strandat 5817.039 kb on - strandat 5817.091 kb on - strandat 5817.123 kb on + strandat 5817.149 kb on + strandat 5817.241 kb on - strand, within BT4413at 5817.248 kb on - strand, within BT4413at 5817.248 kb on - strand, within BT4413at 5817.300 kb on + strand, within BT4413at 5817.300 kb on + strand, within BT4413at 5817.306 kb on + strand, within BT4413at 5817.342 kb on + strand, within BT4413at 5817.424 kb on - strand, within BT4413at 5817.435 kb on - strand, within BT4413at 5817.435 kb on - strand, within BT4413at 5817.457 kb on - strand, within BT4413at 5817.511 kb on - strand, within BT4413at 5817.511 kb on - strandat 5817.527 kb on - strandat 5817.527 kb on - strandat 5817.527 kb on - strandat 5817.527 kb on - strandat 5817.529 kb on + strandat 5817.535 kb on + strandat 5817.535 kb on + strandat 5817.537 kb on + strandat 5817.538 kb on - strandat 5817.538 kb on - strandat 5817.555 kb on + strandat 5817.614 kb on - strandat 5817.614 kb on - strandat 5817.617 kb on + strandat 5817.626 kb on + strandat 5817.640 kb on - strandat 5817.642 kb on - strandat 5817.715 kb on + strandat 5817.716 kb on - strandat 5817.833 kb on - strandat 5817.873 kb on - strand, within BT4414at 5817.873 kb on - strand, within BT4414at 5818.048 kb on - strand, within BT4414at 5818.188 kb on - strand, within BT4414at 5818.236 kb on - strand, within BT4414at 5818.347 kb on + strandat 5818.458 kb on + strandat 5818.459 kb on - strandat 5818.491 kb on + strand, within BT4415at 5818.492 kb on - strand, within BT4415at 5818.561 kb on - strand, within BT4415at 5818.566 kb on - strand, within BT4415at 5818.628 kb on + strand, within BT4415at 5818.674 kb on + strand, within BT4415at 5818.676 kb on + strand, within BT4415at 5818.751 kb on + strand, within BT4415at 5818.763 kb on + strand, within BT4415at 5818.764 kb on - strand, within BT4415at 5818.846 kb on - strand, within BT4415at 5818.970 kb on - strand, within BT4415at 5818.982 kb on + strand, within BT4415at 5819.010 kb on + strand, within BT4415at 5819.010 kb on + strand, within BT4415at 5819.011 kb on - strand, within BT4415at 5819.042 kb on - strand, within BT4415at 5819.172 kb on + strand, within BT4415at 5819.173 kb on - strand, within BT4415at 5819.176 kb on + strand, within BT4415at 5819.218 kb on - strand, within BT4415at 5819.294 kb on + strand, within BT4415

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM
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5,816,815 + +1.3
5,816,944 + -2.4
5,816,992 + -0.4
5,816,992 + -1.0
5,816,993 - -3.0
5,817,025 - -0.4
5,817,028 - -1.0
5,817,036 + +2.0
5,817,039 - -1.9
5,817,091 - +2.0
5,817,123 + -2.1
5,817,149 + -2.0
5,817,241 - BT4413 0.27 -2.4
5,817,248 - BT4413 0.28 -3.4
5,817,248 - BT4413 0.28 +1.0
5,817,300 + BT4413 0.40 -0.9
5,817,300 + BT4413 0.40 -1.6
5,817,306 + BT4413 0.42 -1.2
5,817,342 + BT4413 0.50 -0.8
5,817,424 - BT4413 0.68 +3.0
5,817,435 - BT4413 0.71 +0.5
5,817,435 - BT4413 0.71 -0.3
5,817,457 - BT4413 0.76 -0.0
5,817,511 - BT4413 0.88 -1.0
5,817,511 - -1.0
5,817,527 - -0.0
5,817,527 - -1.5
5,817,527 - -1.6
5,817,527 - -1.0
5,817,529 + -1.0
5,817,535 + -2.0
5,817,535 + -0.0
5,817,537 + -3.9
5,817,538 - +1.5
5,817,538 - +1.2
5,817,555 + +0.8
5,817,614 - +1.0
5,817,614 - -1.0
5,817,617 + +2.5
5,817,626 + +2.3
5,817,640 - -0.8
5,817,642 - -3.9
5,817,715 + -1.9
5,817,716 - -2.4
5,817,833 - -0.0
5,817,873 - BT4414 0.16 -2.7
5,817,873 - BT4414 0.16 -3.5
5,818,048 - BT4414 0.48 +2.0
5,818,188 - BT4414 0.74 +0.5
5,818,236 - BT4414 0.82 -4.2
5,818,347 + +3.1
5,818,458 + -0.0
5,818,459 - +1.0
5,818,491 + BT4415 0.12 -2.0
5,818,492 - BT4415 0.12 +1.0
5,818,561 - BT4415 0.18 -1.0
5,818,566 - BT4415 0.18 +1.0
5,818,628 + BT4415 0.23 -1.3
5,818,674 + BT4415 0.27 +2.3
5,818,676 + BT4415 0.27 -2.5
5,818,751 + BT4415 0.33 -1.5
5,818,763 + BT4415 0.34 -0.7
5,818,764 - BT4415 0.34 -0.3
5,818,846 - BT4415 0.41 -0.0
5,818,970 - BT4415 0.51 -0.0
5,818,982 + BT4415 0.52 +2.8
5,819,010 + BT4415 0.54 +2.5
5,819,010 + BT4415 0.54 -1.8
5,819,011 - BT4415 0.55 +0.5
5,819,042 - BT4415 0.57 -0.6
5,819,172 + BT4415 0.68 +3.5
5,819,173 - BT4415 0.68 -2.3
5,819,176 + BT4415 0.68 +2.3
5,819,218 - BT4415 0.72 +1.3
5,819,294 + BT4415 0.78 +1.9

Or see this region's nucleotide sequence