Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4412 and BT4413 are separated by 83 nucleotides BT4413 and BT4414 are separated by 220 nucleotides BT4414 and BT4415 are separated by 14 nucleotides
BT4412: BT4412 - conserved hypothetical protein (NCBI ptt file), at 5,814,323 to 5,817,040
BT4412
BT4413: BT4413 - hypothetical protein (NCBI ptt file), at 5,817,124 to 5,817,561
BT4413
BT4414: BT4414 - hypothetical protein (NCBI ptt file), at 5,817,782 to 5,818,333
BT4414
BT4415: BT4415 - putative decarboxylase beta subunit (NCBI ptt file), at 5,818,348 to 5,819,562
BT4415
Position (kb)
5817
5818
5819 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5816.815 kb on + strand at 5816.944 kb on + strand at 5816.992 kb on + strand at 5816.992 kb on + strand at 5816.993 kb on - strand at 5817.025 kb on - strand at 5817.028 kb on - strand at 5817.036 kb on + strand at 5817.039 kb on - strand at 5817.091 kb on - strand at 5817.123 kb on + strand at 5817.149 kb on + strand at 5817.241 kb on - strand, within BT4413 at 5817.248 kb on - strand, within BT4413 at 5817.248 kb on - strand, within BT4413 at 5817.300 kb on + strand, within BT4413 at 5817.300 kb on + strand, within BT4413 at 5817.306 kb on + strand, within BT4413 at 5817.342 kb on + strand, within BT4413 at 5817.424 kb on - strand, within BT4413 at 5817.435 kb on - strand, within BT4413 at 5817.435 kb on - strand, within BT4413 at 5817.457 kb on - strand, within BT4413 at 5817.511 kb on - strand, within BT4413 at 5817.511 kb on - strand at 5817.527 kb on - strand at 5817.527 kb on - strand at 5817.527 kb on - strand at 5817.527 kb on - strand at 5817.529 kb on + strand at 5817.535 kb on + strand at 5817.535 kb on + strand at 5817.537 kb on + strand at 5817.538 kb on - strand at 5817.538 kb on - strand at 5817.555 kb on + strand at 5817.614 kb on - strand at 5817.614 kb on - strand at 5817.617 kb on + strand at 5817.626 kb on + strand at 5817.640 kb on - strand at 5817.642 kb on - strand at 5817.715 kb on + strand at 5817.716 kb on - strand at 5817.833 kb on - strand at 5817.873 kb on - strand, within BT4414 at 5817.873 kb on - strand, within BT4414 at 5818.048 kb on - strand, within BT4414 at 5818.188 kb on - strand, within BT4414 at 5818.236 kb on - strand, within BT4414 at 5818.347 kb on + strand at 5818.458 kb on + strand at 5818.459 kb on - strand at 5818.491 kb on + strand, within BT4415 at 5818.492 kb on - strand, within BT4415 at 5818.561 kb on - strand, within BT4415 at 5818.566 kb on - strand, within BT4415 at 5818.628 kb on + strand, within BT4415 at 5818.674 kb on + strand, within BT4415 at 5818.676 kb on + strand, within BT4415 at 5818.751 kb on + strand, within BT4415 at 5818.763 kb on + strand, within BT4415 at 5818.764 kb on - strand, within BT4415 at 5818.846 kb on - strand, within BT4415 at 5818.970 kb on - strand, within BT4415 at 5818.982 kb on + strand, within BT4415 at 5819.010 kb on + strand, within BT4415 at 5819.010 kb on + strand, within BT4415 at 5819.011 kb on - strand, within BT4415 at 5819.042 kb on - strand, within BT4415 at 5819.172 kb on + strand, within BT4415 at 5819.173 kb on - strand, within BT4415 at 5819.176 kb on + strand, within BT4415 at 5819.218 kb on - strand, within BT4415 at 5819.294 kb on + strand, within BT4415
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 5,816,815 + +1.3 5,816,944 + -2.4 5,816,992 + -0.4 5,816,992 + -1.0 5,816,993 - -3.0 5,817,025 - -0.4 5,817,028 - -1.0 5,817,036 + +2.0 5,817,039 - -1.9 5,817,091 - +2.0 5,817,123 + -2.1 5,817,149 + -2.0 5,817,241 - BT4413 0.27 -2.4 5,817,248 - BT4413 0.28 -3.4 5,817,248 - BT4413 0.28 +1.0 5,817,300 + BT4413 0.40 -0.9 5,817,300 + BT4413 0.40 -1.6 5,817,306 + BT4413 0.42 -1.2 5,817,342 + BT4413 0.50 -0.8 5,817,424 - BT4413 0.68 +3.0 5,817,435 - BT4413 0.71 +0.5 5,817,435 - BT4413 0.71 -0.3 5,817,457 - BT4413 0.76 -0.0 5,817,511 - BT4413 0.88 -1.0 5,817,511 - -1.0 5,817,527 - -0.0 5,817,527 - -1.5 5,817,527 - -1.6 5,817,527 - -1.0 5,817,529 + -1.0 5,817,535 + -2.0 5,817,535 + -0.0 5,817,537 + -3.9 5,817,538 - +1.5 5,817,538 - +1.2 5,817,555 + +0.8 5,817,614 - +1.0 5,817,614 - -1.0 5,817,617 + +2.5 5,817,626 + +2.3 5,817,640 - -0.8 5,817,642 - -3.9 5,817,715 + -1.9 5,817,716 - -2.4 5,817,833 - -0.0 5,817,873 - BT4414 0.16 -2.7 5,817,873 - BT4414 0.16 -3.5 5,818,048 - BT4414 0.48 +2.0 5,818,188 - BT4414 0.74 +0.5 5,818,236 - BT4414 0.82 -4.2 5,818,347 + +3.1 5,818,458 + -0.0 5,818,459 - +1.0 5,818,491 + BT4415 0.12 -2.0 5,818,492 - BT4415 0.12 +1.0 5,818,561 - BT4415 0.18 -1.0 5,818,566 - BT4415 0.18 +1.0 5,818,628 + BT4415 0.23 -1.3 5,818,674 + BT4415 0.27 +2.3 5,818,676 + BT4415 0.27 -2.5 5,818,751 + BT4415 0.33 -1.5 5,818,763 + BT4415 0.34 -0.7 5,818,764 - BT4415 0.34 -0.3 5,818,846 - BT4415 0.41 -0.0 5,818,970 - BT4415 0.51 -0.0 5,818,982 + BT4415 0.52 +2.8 5,819,010 + BT4415 0.54 +2.5 5,819,010 + BT4415 0.54 -1.8 5,819,011 - BT4415 0.55 +0.5 5,819,042 - BT4415 0.57 -0.6 5,819,172 + BT4415 0.68 +3.5 5,819,173 - BT4415 0.68 -2.3 5,819,176 + BT4415 0.68 +2.3 5,819,218 - BT4415 0.72 +1.3 5,819,294 + BT4415 0.78 +1.9
Or see this region's nucleotide sequence