Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4269 and BT4270 are separated by 13 nucleotides BT4270 and BT4271 are separated by 112 nucleotides BT4271 and BT4272 are separated by 5 nucleotides
BT4269: BT4269 - hypothetical protein (NCBI ptt file), at 5,624,885 to 5,625,880
BT4269
BT4270: BT4270 - hypothetical protein (NCBI ptt file), at 5,625,894 to 5,627,711
BT4270
BT4271: BT4271 - hypothetical protein (NCBI ptt file), at 5,627,824 to 5,628,531
BT4271
BT4272: BT4272 - hypothetical protein (NCBI ptt file), at 5,628,537 to 5,631,527
BT4272
Position (kb)
5625
5626
5627
5628 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5624.895 kb on + strand at 5624.895 kb on + strand at 5624.945 kb on - strand at 5625.012 kb on - strand, within BT4269 at 5625.170 kb on - strand, within BT4269 at 5625.170 kb on - strand, within BT4269 at 5625.207 kb on - strand, within BT4269 at 5625.321 kb on - strand, within BT4269 at 5625.323 kb on - strand, within BT4269 at 5625.389 kb on - strand, within BT4269 at 5625.398 kb on + strand, within BT4269 at 5625.443 kb on - strand, within BT4269 at 5625.451 kb on + strand, within BT4269 at 5625.452 kb on - strand, within BT4269 at 5625.570 kb on - strand, within BT4269 at 5625.570 kb on - strand, within BT4269 at 5625.570 kb on - strand, within BT4269 at 5625.659 kb on - strand, within BT4269 at 5625.661 kb on + strand, within BT4269 at 5625.662 kb on - strand, within BT4269 at 5625.671 kb on + strand, within BT4269 at 5625.674 kb on - strand, within BT4269 at 5625.721 kb on + strand, within BT4269 at 5625.721 kb on + strand, within BT4269 at 5625.726 kb on - strand, within BT4269 at 5625.768 kb on - strand, within BT4269 at 5625.772 kb on + strand, within BT4269 at 5625.798 kb on - strand at 5625.812 kb on - strand at 5625.832 kb on - strand at 5625.849 kb on - strand at 5625.894 kb on - strand at 5626.091 kb on + strand, within BT4270 at 5626.092 kb on - strand, within BT4270 at 5626.180 kb on - strand, within BT4270 at 5626.344 kb on - strand, within BT4270 at 5626.364 kb on + strand, within BT4270 at 5626.368 kb on - strand, within BT4270 at 5626.379 kb on + strand, within BT4270 at 5626.520 kb on + strand, within BT4270 at 5626.521 kb on - strand, within BT4270 at 5626.594 kb on - strand, within BT4270 at 5626.595 kb on + strand, within BT4270 at 5626.596 kb on - strand, within BT4270 at 5626.626 kb on + strand, within BT4270 at 5626.667 kb on + strand, within BT4270 at 5626.694 kb on + strand, within BT4270 at 5626.694 kb on + strand, within BT4270 at 5626.694 kb on + strand, within BT4270 at 5626.695 kb on - strand, within BT4270 at 5626.695 kb on - strand, within BT4270 at 5626.793 kb on + strand, within BT4270 at 5626.793 kb on + strand, within BT4270 at 5626.794 kb on - strand, within BT4270 at 5626.853 kb on + strand, within BT4270 at 5626.929 kb on - strand, within BT4270 at 5627.054 kb on + strand, within BT4270 at 5627.065 kb on + strand, within BT4270 at 5627.066 kb on - strand, within BT4270 at 5627.066 kb on - strand, within BT4270 at 5627.070 kb on - strand, within BT4270 at 5627.111 kb on + strand, within BT4270 at 5627.124 kb on + strand, within BT4270 at 5627.202 kb on - strand, within BT4270 at 5627.220 kb on - strand, within BT4270 at 5627.284 kb on + strand, within BT4270 at 5627.284 kb on + strand, within BT4270 at 5627.285 kb on - strand, within BT4270 at 5627.391 kb on + strand, within BT4270 at 5627.493 kb on + strand, within BT4270 at 5627.494 kb on - strand, within BT4270 at 5627.495 kb on + strand, within BT4270 at 5627.560 kb on - strand at 5627.565 kb on + strand at 5627.639 kb on + strand at 5627.653 kb on - strand at 5627.659 kb on - strand at 5627.660 kb on + strand at 5627.702 kb on - strand at 5627.721 kb on - strand at 5627.801 kb on - strand at 5627.855 kb on + strand at 5627.989 kb on + strand, within BT4271 at 5627.989 kb on + strand, within BT4271 at 5628.015 kb on + strand at 5628.068 kb on - strand, within BT4271 at 5628.068 kb on - strand, within BT4271 at 5628.180 kb on - strand, within BT4271 at 5628.205 kb on - strand, within BT4271 at 5628.205 kb on - strand, within BT4271 at 5628.398 kb on + strand, within BT4271 at 5628.471 kb on + strand at 5628.565 kb on + strand at 5628.566 kb on - strand at 5628.569 kb on + strand at 5628.570 kb on - strand at 5628.570 kb on - strand at 5628.599 kb on + strand at 5628.606 kb on + strand at 5628.627 kb on - strand at 5628.627 kb on - strand at 5628.703 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 5,624,895 + +2.6 5,624,895 + +1.6 5,624,945 - +0.0 5,625,012 - BT4269 0.13 +1.4 5,625,170 - BT4269 0.29 -0.4 5,625,170 - BT4269 0.29 +0.0 5,625,207 - BT4269 0.32 +0.0 5,625,321 - BT4269 0.44 -3.7 5,625,323 - BT4269 0.44 -2.3 5,625,389 - BT4269 0.51 +0.5 5,625,398 + BT4269 0.52 -1.6 5,625,443 - BT4269 0.56 -2.6 5,625,451 + BT4269 0.57 -1.0 5,625,452 - BT4269 0.57 -2.4 5,625,570 - BT4269 0.69 +3.0 5,625,570 - BT4269 0.69 -1.1 5,625,570 - BT4269 0.69 -3.3 5,625,659 - BT4269 0.78 +2.6 5,625,661 + BT4269 0.78 +2.3 5,625,662 - BT4269 0.78 +1.2 5,625,671 + BT4269 0.79 -0.9 5,625,674 - BT4269 0.79 -1.8 5,625,721 + BT4269 0.84 +1.0 5,625,721 + BT4269 0.84 -0.6 5,625,726 - BT4269 0.84 -2.0 5,625,768 - BT4269 0.89 -0.6 5,625,772 + BT4269 0.89 +0.0 5,625,798 - -2.6 5,625,812 - -2.0 5,625,832 - +2.0 5,625,849 - -1.8 5,625,894 - +0.6 5,626,091 + BT4270 0.11 -1.4 5,626,092 - BT4270 0.11 -0.6 5,626,180 - BT4270 0.16 -2.6 5,626,344 - BT4270 0.25 -0.5 5,626,364 + BT4270 0.26 +2.8 5,626,368 - BT4270 0.26 -0.5 5,626,379 + BT4270 0.27 +0.6 5,626,520 + BT4270 0.34 -1.0 5,626,521 - BT4270 0.34 +0.0 5,626,594 - BT4270 0.39 +2.3 5,626,595 + BT4270 0.39 -2.0 5,626,596 - BT4270 0.39 -1.6 5,626,626 + BT4270 0.40 -2.7 5,626,667 + BT4270 0.43 -1.7 5,626,694 + BT4270 0.44 -2.0 5,626,694 + BT4270 0.44 -2.8 5,626,694 + BT4270 0.44 +2.8 5,626,695 - BT4270 0.44 +0.0 5,626,695 - BT4270 0.44 +0.0 5,626,793 + BT4270 0.49 +1.0 5,626,793 + BT4270 0.49 -2.3 5,626,794 - BT4270 0.50 +2.3 5,626,853 + BT4270 0.53 -2.2 5,626,929 - BT4270 0.57 -1.2 5,627,054 + BT4270 0.64 +0.0 5,627,065 + BT4270 0.64 -0.2 5,627,066 - BT4270 0.64 -2.0 5,627,066 - BT4270 0.64 -1.6 5,627,070 - BT4270 0.65 -1.5 5,627,111 + BT4270 0.67 -2.6 5,627,124 + BT4270 0.68 -2.6 5,627,202 - BT4270 0.72 -2.2 5,627,220 - BT4270 0.73 -2.8 5,627,284 + BT4270 0.76 +1.3 5,627,284 + BT4270 0.76 -3.6 5,627,285 - BT4270 0.77 +1.3 5,627,391 + BT4270 0.82 -1.9 5,627,493 + BT4270 0.88 -2.6 5,627,494 - BT4270 0.88 -3.0 5,627,495 + BT4270 0.88 -1.9 5,627,560 - -1.7 5,627,565 + -1.6 5,627,639 + -2.6 5,627,653 - -0.9 5,627,659 - -2.1 5,627,660 + +3.3 5,627,702 - +2.3 5,627,721 - -0.8 5,627,801 - +2.6 5,627,855 + +0.0 5,627,989 + BT4271 0.23 +0.0 5,627,989 + BT4271 0.23 +1.6 5,628,015 + -1.6 5,628,068 - BT4271 0.34 -1.0 5,628,068 - BT4271 0.34 -2.3 5,628,180 - BT4271 0.50 +0.8 5,628,205 - BT4271 0.54 -2.2 5,628,205 - BT4271 0.54 +1.0 5,628,398 + BT4271 0.81 +1.6 5,628,471 + -1.8 5,628,565 + +3.3 5,628,566 - -2.0 5,628,569 + -0.6 5,628,570 - -2.6 5,628,570 - +1.7 5,628,599 + -3.9 5,628,606 + +0.0 5,628,627 - -3.3 5,628,627 - -1.9 5,628,703 + -1.0
Or see this region's nucleotide sequence