Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4218 and BT4219 are separated by 90 nucleotides BT4219 and BT4220 are separated by 83 nucleotides
BT4218: BT4218 - phosphate starvation-inducible protein, PhoH (NCBI ptt file), at 5,554,609 to 5,555,625
BT4218
BT4219: BT4219 - hypothetical protein (NCBI ptt file), at 5,555,716 to 5,556,393
BT4219
BT4220: BT4220 - flotillin-like protein (NCBI ptt file), at 5,556,477 to 5,558,135
BT4220
Position (kb) 5555
5556
5557 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5554.718 kb on - strand, within BT4218 at 5554.858 kb on - strand, within BT4218 at 5554.930 kb on - strand, within BT4218 at 5554.984 kb on - strand, within BT4218 at 5555.070 kb on - strand, within BT4218 at 5555.072 kb on - strand, within BT4218 at 5555.085 kb on - strand, within BT4218 at 5555.255 kb on - strand, within BT4218 at 5555.386 kb on - strand, within BT4218 at 5555.525 kb on - strand at 5555.537 kb on - strand at 5555.537 kb on - strand at 5555.537 kb on - strand at 5555.549 kb on - strand at 5555.701 kb on + strand at 5555.701 kb on + strand at 5555.757 kb on - strand at 5555.762 kb on - strand at 5555.762 kb on - strand at 5555.980 kb on + strand, within BT4219 at 5556.089 kb on + strand, within BT4219 at 5556.116 kb on - strand, within BT4219 at 5556.257 kb on + strand, within BT4219 at 5556.257 kb on + strand, within BT4219 at 5556.258 kb on - strand, within BT4219 at 5556.306 kb on + strand, within BT4219 at 5556.358 kb on - strand at 5556.358 kb on - strand at 5556.382 kb on - strand at 5556.460 kb on - strand at 5556.460 kb on - strand at 5556.592 kb on - strand at 5556.592 kb on - strand at 5556.644 kb on + strand, within BT4220 at 5556.645 kb on - strand, within BT4220 at 5556.739 kb on - strand, within BT4220 at 5556.739 kb on - strand, within BT4220 at 5556.739 kb on - strand, within BT4220 at 5556.791 kb on + strand, within BT4220 at 5557.077 kb on + strand, within BT4220 at 5557.185 kb on - strand, within BT4220
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 5,554,718 - BT4218 0.11 +3.8 5,554,858 - BT4218 0.24 -1.6 5,554,930 - BT4218 0.32 -1.0 5,554,984 - BT4218 0.37 -1.6 5,555,070 - BT4218 0.45 +0.5 5,555,072 - BT4218 0.46 -1.1 5,555,085 - BT4218 0.47 +3.0 5,555,255 - BT4218 0.64 -0.2 5,555,386 - BT4218 0.76 +0.1 5,555,525 - -1.6 5,555,537 - -0.4 5,555,537 - -0.8 5,555,537 - -1.8 5,555,549 - -1.2 5,555,701 + +0.6 5,555,701 + -2.0 5,555,757 - -1.3 5,555,762 - +0.0 5,555,762 - -2.0 5,555,980 + BT4219 0.39 -1.0 5,556,089 + BT4219 0.55 -3.2 5,556,116 - BT4219 0.59 +1.6 5,556,257 + BT4219 0.80 -1.6 5,556,257 + BT4219 0.80 -1.9 5,556,258 - BT4219 0.80 -2.0 5,556,306 + BT4219 0.87 -0.6 5,556,358 - +0.5 5,556,358 - -3.2 5,556,382 - -0.8 5,556,460 - -1.5 5,556,460 - -2.4 5,556,592 - +2.0 5,556,592 - -0.6 5,556,644 + BT4220 0.10 -1.7 5,556,645 - BT4220 0.10 -0.6 5,556,739 - BT4220 0.16 -2.6 5,556,739 - BT4220 0.16 +0.0 5,556,739 - BT4220 0.16 -0.6 5,556,791 + BT4220 0.19 -1.0 5,557,077 + BT4220 0.36 +0.0 5,557,185 - BT4220 0.43 +3.2
Or see this region's nucleotide sequence