Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4009 and BT4010 are separated by 228 nucleotides BT4010 and BT4011 are separated by 4 nucleotides
BT4009: BT4009 - integrase (NCBI ptt file), at 5,222,847 to 5,223,959
BT4009
BT4010: BT4010 - conserved hypothetical protein (NCBI ptt file), at 5,224,188 to 5,225,576
BT4010
BT4011: BT4011 - DNA-methyltransferase (NCBI ptt file), at 5,225,581 to 5,226,441
BT4011
Position (kb)
5224
5225
5226 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5223.280 kb on + strand, within BT4009 at 5223.409 kb on + strand, within BT4009 at 5223.582 kb on - strand, within BT4009 at 5223.610 kb on + strand, within BT4009 at 5223.613 kb on - strand, within BT4009 at 5223.614 kb on + strand, within BT4009 at 5223.614 kb on + strand, within BT4009 at 5223.627 kb on + strand, within BT4009 at 5223.718 kb on + strand, within BT4009 at 5223.736 kb on + strand, within BT4009 at 5223.757 kb on + strand, within BT4009 at 5223.758 kb on - strand, within BT4009 at 5223.758 kb on - strand, within BT4009 at 5223.760 kb on + strand, within BT4009 at 5223.805 kb on + strand, within BT4009 at 5223.805 kb on + strand, within BT4009 at 5223.840 kb on + strand, within BT4009 at 5223.841 kb on - strand, within BT4009 at 5223.841 kb on - strand, within BT4009 at 5223.841 kb on - strand, within BT4009 at 5223.872 kb on - strand at 5223.875 kb on + strand at 5224.012 kb on + strand at 5224.013 kb on - strand at 5224.047 kb on - strand at 5224.065 kb on - strand at 5224.131 kb on - strand at 5224.195 kb on + strand at 5224.276 kb on + strand at 5224.304 kb on + strand at 5224.391 kb on + strand, within BT4010 at 5224.391 kb on + strand, within BT4010 at 5224.395 kb on + strand, within BT4010 at 5224.457 kb on - strand, within BT4010 at 5224.459 kb on + strand, within BT4010 at 5224.517 kb on + strand, within BT4010 at 5224.523 kb on + strand, within BT4010 at 5224.580 kb on + strand, within BT4010 at 5224.581 kb on - strand, within BT4010 at 5224.596 kb on - strand, within BT4010 at 5224.613 kb on + strand, within BT4010 at 5224.760 kb on + strand, within BT4010 at 5224.803 kb on + strand, within BT4010 at 5224.804 kb on - strand, within BT4010 at 5224.859 kb on + strand, within BT4010 at 5224.888 kb on + strand, within BT4010 at 5224.962 kb on + strand, within BT4010 at 5225.149 kb on + strand, within BT4010 at 5225.149 kb on + strand, within BT4010 at 5225.150 kb on - strand, within BT4010 at 5225.153 kb on + strand, within BT4010 at 5225.191 kb on + strand, within BT4010 at 5225.191 kb on + strand, within BT4010 at 5225.239 kb on + strand, within BT4010 at 5225.271 kb on + strand, within BT4010 at 5225.306 kb on + strand, within BT4010 at 5225.307 kb on - strand, within BT4010 at 5225.351 kb on + strand, within BT4010 at 5225.377 kb on + strand, within BT4010 at 5225.398 kb on + strand, within BT4010 at 5225.398 kb on + strand, within BT4010 at 5225.398 kb on + strand, within BT4010 at 5225.410 kb on + strand, within BT4010 at 5225.466 kb on + strand at 5225.466 kb on + strand at 5225.493 kb on - strand at 5225.527 kb on + strand at 5226.499 kb on + strand at 5226.499 kb on + strand at 5226.500 kb on - strand at 5226.554 kb on - strand at 5226.554 kb on - strand at 5226.554 kb on - strand at 5226.563 kb on + strand at 5226.565 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 5,223,280 + BT4009 0.39 -0.5 5,223,409 + BT4009 0.50 -1.3 5,223,582 - BT4009 0.66 -2.4 5,223,610 + BT4009 0.69 -0.1 5,223,613 - BT4009 0.69 -1.6 5,223,614 + BT4009 0.69 -2.6 5,223,614 + BT4009 0.69 +0.4 5,223,627 + BT4009 0.70 +0.9 5,223,718 + BT4009 0.78 -0.1 5,223,736 + BT4009 0.80 -1.1 5,223,757 + BT4009 0.82 -2.5 5,223,758 - BT4009 0.82 -0.1 5,223,758 - BT4009 0.82 -1.1 5,223,760 + BT4009 0.82 -4.1 5,223,805 + BT4009 0.86 +2.9 5,223,805 + BT4009 0.86 -1.9 5,223,840 + BT4009 0.89 -1.3 5,223,841 - BT4009 0.89 -0.5 5,223,841 - BT4009 0.89 -0.1 5,223,841 - BT4009 0.89 -1.6 5,223,872 - +0.4 5,223,875 + +3.7 5,224,012 + -2.0 5,224,013 - +0.9 5,224,047 - -2.6 5,224,065 - -0.1 5,224,131 - -1.4 5,224,195 + -0.4 5,224,276 + -2.9 5,224,304 + -0.1 5,224,391 + BT4010 0.15 -0.8 5,224,391 + BT4010 0.15 -3.2 5,224,395 + BT4010 0.15 -1.3 5,224,457 - BT4010 0.19 -2.6 5,224,459 + BT4010 0.20 +1.6 5,224,517 + BT4010 0.24 +0.9 5,224,523 + BT4010 0.24 -0.5 5,224,580 + BT4010 0.28 +1.5 5,224,581 - BT4010 0.28 -0.1 5,224,596 - BT4010 0.29 -2.6 5,224,613 + BT4010 0.31 -3.4 5,224,760 + BT4010 0.41 -1.3 5,224,803 + BT4010 0.44 +2.5 5,224,804 - BT4010 0.44 -0.6 5,224,859 + BT4010 0.48 -0.6 5,224,888 + BT4010 0.50 -0.8 5,224,962 + BT4010 0.56 +1.1 5,225,149 + BT4010 0.69 -0.8 5,225,149 + BT4010 0.69 +2.4 5,225,150 - BT4010 0.69 -0.3 5,225,153 + BT4010 0.69 -2.6 5,225,191 + BT4010 0.72 -2.1 5,225,191 + BT4010 0.72 -1.6 5,225,239 + BT4010 0.76 -2.6 5,225,271 + BT4010 0.78 +1.9 5,225,306 + BT4010 0.80 +0.2 5,225,307 - BT4010 0.81 -1.4 5,225,351 + BT4010 0.84 +1.5 5,225,377 + BT4010 0.86 -0.4 5,225,398 + BT4010 0.87 +0.3 5,225,398 + BT4010 0.87 +1.4 5,225,398 + BT4010 0.87 -0.8 5,225,410 + BT4010 0.88 +1.7 5,225,466 + -1.1 5,225,466 + -0.2 5,225,493 - +2.4 5,225,527 + -0.1 5,226,499 + -1.4 5,226,499 + -1.9 5,226,500 - +0.5 5,226,554 - +3.1 5,226,554 - -3.1 5,226,554 - -2.0 5,226,563 + -0.7 5,226,565 + -1.4
Or see this region's nucleotide sequence