Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4010

Experiment: BHIS with 5-Fluorouracil 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4009 and BT4010 are separated by 228 nucleotidesBT4010 and BT4011 are separated by 4 nucleotides BT4009: BT4009 - integrase (NCBI ptt file), at 5,222,847 to 5,223,959 BT4009 BT4010: BT4010 - conserved hypothetical protein (NCBI ptt file), at 5,224,188 to 5,225,576 BT4010 BT4011: BT4011 - DNA-methyltransferase (NCBI ptt file), at 5,225,581 to 5,226,441 BT4011 Position (kb) 5224 5225 5226Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5223.280 kb on + strand, within BT4009at 5223.409 kb on + strand, within BT4009at 5223.582 kb on - strand, within BT4009at 5223.610 kb on + strand, within BT4009at 5223.613 kb on - strand, within BT4009at 5223.614 kb on + strand, within BT4009at 5223.614 kb on + strand, within BT4009at 5223.627 kb on + strand, within BT4009at 5223.718 kb on + strand, within BT4009at 5223.736 kb on + strand, within BT4009at 5223.757 kb on + strand, within BT4009at 5223.758 kb on - strand, within BT4009at 5223.758 kb on - strand, within BT4009at 5223.760 kb on + strand, within BT4009at 5223.805 kb on + strand, within BT4009at 5223.805 kb on + strand, within BT4009at 5223.840 kb on + strand, within BT4009at 5223.841 kb on - strand, within BT4009at 5223.841 kb on - strand, within BT4009at 5223.841 kb on - strand, within BT4009at 5223.872 kb on - strandat 5223.875 kb on + strandat 5224.012 kb on + strandat 5224.013 kb on - strandat 5224.047 kb on - strandat 5224.065 kb on - strandat 5224.131 kb on - strandat 5224.195 kb on + strandat 5224.276 kb on + strandat 5224.304 kb on + strandat 5224.391 kb on + strand, within BT4010at 5224.391 kb on + strand, within BT4010at 5224.395 kb on + strand, within BT4010at 5224.457 kb on - strand, within BT4010at 5224.459 kb on + strand, within BT4010at 5224.517 kb on + strand, within BT4010at 5224.523 kb on + strand, within BT4010at 5224.580 kb on + strand, within BT4010at 5224.581 kb on - strand, within BT4010at 5224.596 kb on - strand, within BT4010at 5224.613 kb on + strand, within BT4010at 5224.760 kb on + strand, within BT4010at 5224.803 kb on + strand, within BT4010at 5224.804 kb on - strand, within BT4010at 5224.859 kb on + strand, within BT4010at 5224.888 kb on + strand, within BT4010at 5224.962 kb on + strand, within BT4010at 5225.149 kb on + strand, within BT4010at 5225.149 kb on + strand, within BT4010at 5225.150 kb on - strand, within BT4010at 5225.153 kb on + strand, within BT4010at 5225.191 kb on + strand, within BT4010at 5225.191 kb on + strand, within BT4010at 5225.239 kb on + strand, within BT4010at 5225.271 kb on + strand, within BT4010at 5225.306 kb on + strand, within BT4010at 5225.307 kb on - strand, within BT4010at 5225.351 kb on + strand, within BT4010at 5225.377 kb on + strand, within BT4010at 5225.398 kb on + strand, within BT4010at 5225.398 kb on + strand, within BT4010at 5225.398 kb on + strand, within BT4010at 5225.410 kb on + strand, within BT4010at 5225.466 kb on + strandat 5225.466 kb on + strandat 5225.493 kb on - strandat 5225.527 kb on + strandat 5226.499 kb on + strandat 5226.499 kb on + strandat 5226.500 kb on - strandat 5226.554 kb on - strandat 5226.554 kb on - strandat 5226.554 kb on - strandat 5226.563 kb on + strandat 5226.565 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM
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5,223,280 + BT4009 0.39 -0.5
5,223,409 + BT4009 0.50 -1.3
5,223,582 - BT4009 0.66 -2.4
5,223,610 + BT4009 0.69 -0.1
5,223,613 - BT4009 0.69 -1.6
5,223,614 + BT4009 0.69 -2.6
5,223,614 + BT4009 0.69 +0.4
5,223,627 + BT4009 0.70 +0.9
5,223,718 + BT4009 0.78 -0.1
5,223,736 + BT4009 0.80 -1.1
5,223,757 + BT4009 0.82 -2.5
5,223,758 - BT4009 0.82 -0.1
5,223,758 - BT4009 0.82 -1.1
5,223,760 + BT4009 0.82 -4.1
5,223,805 + BT4009 0.86 +2.9
5,223,805 + BT4009 0.86 -1.9
5,223,840 + BT4009 0.89 -1.3
5,223,841 - BT4009 0.89 -0.5
5,223,841 - BT4009 0.89 -0.1
5,223,841 - BT4009 0.89 -1.6
5,223,872 - +0.4
5,223,875 + +3.7
5,224,012 + -2.0
5,224,013 - +0.9
5,224,047 - -2.6
5,224,065 - -0.1
5,224,131 - -1.4
5,224,195 + -0.4
5,224,276 + -2.9
5,224,304 + -0.1
5,224,391 + BT4010 0.15 -0.8
5,224,391 + BT4010 0.15 -3.2
5,224,395 + BT4010 0.15 -1.3
5,224,457 - BT4010 0.19 -2.6
5,224,459 + BT4010 0.20 +1.6
5,224,517 + BT4010 0.24 +0.9
5,224,523 + BT4010 0.24 -0.5
5,224,580 + BT4010 0.28 +1.5
5,224,581 - BT4010 0.28 -0.1
5,224,596 - BT4010 0.29 -2.6
5,224,613 + BT4010 0.31 -3.4
5,224,760 + BT4010 0.41 -1.3
5,224,803 + BT4010 0.44 +2.5
5,224,804 - BT4010 0.44 -0.6
5,224,859 + BT4010 0.48 -0.6
5,224,888 + BT4010 0.50 -0.8
5,224,962 + BT4010 0.56 +1.1
5,225,149 + BT4010 0.69 -0.8
5,225,149 + BT4010 0.69 +2.4
5,225,150 - BT4010 0.69 -0.3
5,225,153 + BT4010 0.69 -2.6
5,225,191 + BT4010 0.72 -2.1
5,225,191 + BT4010 0.72 -1.6
5,225,239 + BT4010 0.76 -2.6
5,225,271 + BT4010 0.78 +1.9
5,225,306 + BT4010 0.80 +0.2
5,225,307 - BT4010 0.81 -1.4
5,225,351 + BT4010 0.84 +1.5
5,225,377 + BT4010 0.86 -0.4
5,225,398 + BT4010 0.87 +0.3
5,225,398 + BT4010 0.87 +1.4
5,225,398 + BT4010 0.87 -0.8
5,225,410 + BT4010 0.88 +1.7
5,225,466 + -1.1
5,225,466 + -0.2
5,225,493 - +2.4
5,225,527 + -0.1
5,226,499 + -1.4
5,226,499 + -1.9
5,226,500 - +0.5
5,226,554 - +3.1
5,226,554 - -3.1
5,226,554 - -2.0
5,226,563 + -0.7
5,226,565 + -1.4

Or see this region's nucleotide sequence