Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2587 and BT2588 are separated by 245 nucleotides BT2588 and BT2589 are separated by 2 nucleotides BT2589 and BT2590 are separated by 124 nucleotides
BT2587: BT2587 - hypothetical protein (NCBI ptt file), at 3,229,181 to 3,230,146
BT2587
BT2588: BT2588 - conserved hypothetical protein (NCBI ptt file), at 3,230,392 to 3,231,195
BT2588
BT2589: BT2589 - type I restriction enzyme, M subunit (NCBI ptt file), at 3,231,198 to 3,231,968
BT2589
BT2590: BT2590 - hypothetical protein (NCBI ptt file), at 3,232,093 to 3,233,250
BT2590
Position (kb)
3230
3231
3232 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3229.410 kb on + strand, within BT2587 at 3229.410 kb on + strand, within BT2587 at 3229.419 kb on + strand, within BT2587 at 3229.420 kb on - strand, within BT2587 at 3229.420 kb on - strand, within BT2587 at 3229.420 kb on - strand, within BT2587 at 3229.420 kb on - strand, within BT2587 at 3229.421 kb on + strand, within BT2587 at 3229.421 kb on + strand, within BT2587 at 3229.421 kb on + strand, within BT2587 at 3229.423 kb on + strand, within BT2587 at 3229.423 kb on + strand, within BT2587 at 3229.423 kb on + strand, within BT2587 at 3229.441 kb on - strand, within BT2587 at 3229.480 kb on + strand, within BT2587 at 3229.493 kb on + strand, within BT2587 at 3229.511 kb on + strand, within BT2587 at 3229.548 kb on - strand, within BT2587 at 3229.548 kb on - strand, within BT2587 at 3229.566 kb on - strand, within BT2587 at 3229.625 kb on - strand, within BT2587 at 3229.675 kb on + strand, within BT2587 at 3229.694 kb on + strand, within BT2587 at 3229.704 kb on + strand, within BT2587 at 3229.783 kb on + strand, within BT2587 at 3229.784 kb on - strand, within BT2587 at 3229.807 kb on + strand, within BT2587 at 3229.860 kb on + strand, within BT2587 at 3229.860 kb on + strand, within BT2587 at 3229.860 kb on + strand, within BT2587 at 3229.866 kb on + strand, within BT2587 at 3229.866 kb on + strand, within BT2587 at 3229.866 kb on + strand, within BT2587 at 3229.866 kb on + strand, within BT2587 at 3229.867 kb on - strand, within BT2587 at 3229.868 kb on + strand, within BT2587 at 3229.868 kb on + strand, within BT2587 at 3229.868 kb on + strand, within BT2587 at 3229.869 kb on - strand, within BT2587 at 3229.997 kb on + strand, within BT2587 at 3230.004 kb on - strand, within BT2587 at 3230.004 kb on - strand, within BT2587 at 3230.032 kb on - strand, within BT2587 at 3230.032 kb on - strand, within BT2587 at 3230.124 kb on + strand at 3230.129 kb on - strand at 3230.143 kb on - strand at 3230.175 kb on + strand at 3230.254 kb on - strand at 3230.369 kb on - strand at 3230.401 kb on - strand at 3230.411 kb on - strand at 3230.411 kb on - strand at 3230.479 kb on - strand, within BT2588 at 3230.490 kb on - strand, within BT2588 at 3230.687 kb on - strand, within BT2588 at 3230.733 kb on - strand, within BT2588 at 3230.963 kb on - strand, within BT2588 at 3231.024 kb on - strand, within BT2588 at 3231.026 kb on - strand, within BT2588 at 3231.111 kb on - strand, within BT2588 at 3231.117 kb on - strand at 3231.117 kb on - strand at 3231.169 kb on - strand at 3231.169 kb on - strand at 3231.169 kb on - strand at 3231.182 kb on - strand at 3231.208 kb on - strand at 3231.361 kb on - strand, within BT2589 at 3231.441 kb on - strand, within BT2589 at 3231.441 kb on - strand, within BT2589 at 3231.441 kb on - strand, within BT2589 at 3231.470 kb on - strand, within BT2589 at 3231.472 kb on - strand, within BT2589 at 3231.474 kb on - strand, within BT2589 at 3231.530 kb on - strand, within BT2589 at 3231.704 kb on - strand, within BT2589 at 3231.885 kb on - strand, within BT2589 at 3231.885 kb on - strand, within BT2589 at 3231.924 kb on - strand at 3231.924 kb on - strand at 3231.947 kb on - strand at 3231.947 kb on - strand at 3231.983 kb on - strand at 3232.038 kb on + strand at 3232.071 kb on + strand at 3232.080 kb on - strand at 3232.106 kb on + strand at 3232.154 kb on + strand at 3232.154 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 3,229,410 + BT2587 0.24 +1.9 3,229,410 + BT2587 0.24 -2.7 3,229,419 + BT2587 0.25 -1.5 3,229,420 - BT2587 0.25 -1.1 3,229,420 - BT2587 0.25 +2.7 3,229,420 - BT2587 0.25 -1.4 3,229,420 - BT2587 0.25 +0.1 3,229,421 + BT2587 0.25 +2.9 3,229,421 + BT2587 0.25 -1.9 3,229,421 + BT2587 0.25 -1.9 3,229,423 + BT2587 0.25 -1.1 3,229,423 + BT2587 0.25 -1.3 3,229,423 + BT2587 0.25 +1.1 3,229,441 - BT2587 0.27 -1.3 3,229,480 + BT2587 0.31 +0.1 3,229,493 + BT2587 0.32 +1.9 3,229,511 + BT2587 0.34 +1.5 3,229,548 - BT2587 0.38 -1.0 3,229,548 - BT2587 0.38 +0.1 3,229,566 - BT2587 0.40 +0.7 3,229,625 - BT2587 0.46 +1.7 3,229,675 + BT2587 0.51 -1.3 3,229,694 + BT2587 0.53 +2.1 3,229,704 + BT2587 0.54 +0.1 3,229,783 + BT2587 0.62 -3.7 3,229,784 - BT2587 0.62 -0.9 3,229,807 + BT2587 0.65 -2.2 3,229,860 + BT2587 0.70 -0.3 3,229,860 + BT2587 0.70 +3.6 3,229,860 + BT2587 0.70 +2.6 3,229,866 + BT2587 0.71 -2.9 3,229,866 + BT2587 0.71 -0.4 3,229,866 + BT2587 0.71 +1.9 3,229,866 + BT2587 0.71 -0.1 3,229,867 - BT2587 0.71 -1.4 3,229,868 + BT2587 0.71 +0.7 3,229,868 + BT2587 0.71 +2.7 3,229,868 + BT2587 0.71 +3.0 3,229,869 - BT2587 0.71 -2.0 3,229,997 + BT2587 0.84 -0.6 3,230,004 - BT2587 0.85 -1.2 3,230,004 - BT2587 0.85 -1.6 3,230,032 - BT2587 0.88 -4.4 3,230,032 - BT2587 0.88 -2.2 3,230,124 + +1.1 3,230,129 - -0.7 3,230,143 - -0.3 3,230,175 + +2.6 3,230,254 - -2.7 3,230,369 - -1.3 3,230,401 - -1.7 3,230,411 - -2.9 3,230,411 - -0.9 3,230,479 - BT2588 0.11 -0.7 3,230,490 - BT2588 0.12 +2.7 3,230,687 - BT2588 0.37 -1.7 3,230,733 - BT2588 0.42 +1.7 3,230,963 - BT2588 0.71 +2.4 3,231,024 - BT2588 0.79 +2.5 3,231,026 - BT2588 0.79 +2.1 3,231,111 - BT2588 0.89 -2.2 3,231,117 - +2.5 3,231,117 - -2.2 3,231,169 - +2.1 3,231,169 - +0.1 3,231,169 - -1.4 3,231,182 - -0.8 3,231,208 - -0.8 3,231,361 - BT2589 0.21 -1.6 3,231,441 - BT2589 0.32 -1.1 3,231,441 - BT2589 0.32 -0.8 3,231,441 - BT2589 0.32 +2.5 3,231,470 - BT2589 0.35 +2.6 3,231,472 - BT2589 0.36 +2.5 3,231,474 - BT2589 0.36 +2.2 3,231,530 - BT2589 0.43 +2.6 3,231,704 - BT2589 0.66 +2.0 3,231,885 - BT2589 0.89 +0.9 3,231,885 - BT2589 0.89 +2.2 3,231,924 - +0.9 3,231,924 - -2.3 3,231,947 - +1.6 3,231,947 - +1.5 3,231,983 - -1.9 3,232,038 + -0.8 3,232,071 + -2.7 3,232,080 - -1.8 3,232,106 + +0.9 3,232,154 + -2.4 3,232,154 + -2.8
Or see this region's nucleotide sequence