Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1861 and BT1862 are separated by 397 nucleotides BT1862 and BT_r04 are separated by 175 nucleotides BT_r04 and BT_r05 are separated by 418 nucleotides
BT1861: BT1861 - 2-isopropylmalate synthase (EC 2.3.3.13) (from data) , at 2,334,703 to 2,336,199
BT1861
BT1862: BT1862 - hypothetical protein (NCBI ptt file), at 2,336,597 to 2,336,839
BT1862
BT_r04: BT_r04 - 5S ribosomal RNA (RefSeq), at 2,337,015 to 2,337,048
_r04
BT_r05: BT_r05 - 23S ribosomal RNA (RefSeq), at 2,337,467 to 2,340,119
_r05
Position (kb)
2336
2337 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2335.690 kb on - strand, within BT1861 at 2335.698 kb on + strand, within BT1861 at 2335.698 kb on + strand, within BT1861 at 2335.698 kb on + strand, within BT1861 at 2335.698 kb on + strand, within BT1861 at 2335.751 kb on + strand, within BT1861 at 2335.752 kb on - strand, within BT1861 at 2335.817 kb on + strand, within BT1861 at 2335.878 kb on + strand, within BT1861 at 2335.879 kb on - strand, within BT1861 at 2335.908 kb on + strand, within BT1861 at 2335.914 kb on + strand, within BT1861 at 2335.914 kb on + strand, within BT1861 at 2335.914 kb on + strand, within BT1861 at 2335.960 kb on - strand, within BT1861 at 2336.034 kb on + strand, within BT1861 at 2336.181 kb on + strand at 2336.217 kb on + strand at 2336.284 kb on - strand at 2336.327 kb on - strand at 2336.334 kb on + strand at 2336.334 kb on + strand at 2336.344 kb on + strand at 2336.345 kb on - strand at 2336.348 kb on - strand at 2336.348 kb on - strand at 2336.352 kb on + strand at 2336.352 kb on + strand at 2336.353 kb on - strand at 2336.356 kb on + strand at 2336.357 kb on - strand at 2336.358 kb on + strand at 2336.390 kb on - strand at 2336.416 kb on - strand at 2336.519 kb on + strand at 2336.578 kb on - strand at 2336.578 kb on - strand at 2336.586 kb on - strand at 2336.598 kb on + strand at 2336.714 kb on + strand, within BT1862 at 2336.714 kb on + strand, within BT1862 at 2336.758 kb on + strand, within BT1862 at 2336.759 kb on - strand, within BT1862 at 2336.759 kb on - strand, within BT1862 at 2336.762 kb on - strand, within BT1862 at 2336.811 kb on - strand, within BT1862 at 2336.811 kb on - strand, within BT1862 at 2336.840 kb on - strand at 2336.847 kb on - strand at 2336.890 kb on - strand at 2336.891 kb on + strand at 2336.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 2,335,690 - BT1861 0.66 -2.5 2,335,698 + BT1861 0.66 -1.2 2,335,698 + BT1861 0.66 +1.0 2,335,698 + BT1861 0.66 +1.6 2,335,698 + BT1861 0.66 -1.5 2,335,751 + BT1861 0.70 -1.9 2,335,752 - BT1861 0.70 +0.6 2,335,817 + BT1861 0.74 -0.5 2,335,878 + BT1861 0.78 +1.9 2,335,879 - BT1861 0.79 -3.0 2,335,908 + BT1861 0.80 -0.3 2,335,914 + BT1861 0.81 +1.0 2,335,914 + BT1861 0.81 -2.2 2,335,914 + BT1861 0.81 +1.5 2,335,960 - BT1861 0.84 -1.4 2,336,034 + BT1861 0.89 -1.8 2,336,181 + -1.8 2,336,217 + -1.6 2,336,284 - -1.1 2,336,327 - +0.0 2,336,334 + +3.2 2,336,334 + -2.8 2,336,344 + -1.3 2,336,345 - -1.7 2,336,348 - -1.1 2,336,348 - -1.8 2,336,352 + +0.8 2,336,352 + -0.5 2,336,353 - -1.5 2,336,356 + -2.9 2,336,357 - +2.8 2,336,358 + -3.4 2,336,390 - +1.6 2,336,416 - -1.0 2,336,519 + +1.0 2,336,578 - -1.2 2,336,578 - -0.4 2,336,586 - -1.6 2,336,598 + +0.0 2,336,714 + BT1862 0.48 -2.6 2,336,714 + BT1862 0.48 -3.3 2,336,758 + BT1862 0.66 -0.7 2,336,759 - BT1862 0.67 -0.4 2,336,759 - BT1862 0.67 -3.3 2,336,762 - BT1862 0.68 +0.4 2,336,811 - BT1862 0.88 -3.7 2,336,811 - BT1862 0.88 -1.7 2,336,840 - -2.0 2,336,847 - +1.8 2,336,890 - -3.0 2,336,891 + -1.8 2,336,892 - -2.8
Or see this region's nucleotide sequence