Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1729 and BT1730 overlap by 19 nucleotides BT1730 and BT1731 are separated by 0 nucleotides BT1731 and BT1732 are separated by 11 nucleotides
BT1729: BT1729 - peptide chain release factor 3 (RF-3) (NCBI ptt file), at 2,127,615 to 2,129,213
BT1729
BT1730: BT1730 - putative dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 2,129,195 to 2,130,043
BT1730
BT1731: BT1731 - hypothetical protein (NCBI ptt file), at 2,130,044 to 2,130,589
BT1731
BT1732: BT1732 - conserved hypothetical protein (NCBI ptt file), at 2,130,601 to 2,131,254
BT1732
Position (kb)
2129
2130
2131 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2128.214 kb on - strand, within BT1729 at 2128.223 kb on + strand, within BT1729 at 2128.224 kb on - strand, within BT1729 at 2128.224 kb on - strand, within BT1729 at 2128.228 kb on - strand, within BT1729 at 2128.245 kb on + strand, within BT1729 at 2128.246 kb on - strand, within BT1729 at 2128.246 kb on - strand, within BT1729 at 2128.296 kb on - strand, within BT1729 at 2128.301 kb on + strand, within BT1729 at 2128.360 kb on - strand, within BT1729 at 2128.443 kb on + strand, within BT1729 at 2128.575 kb on + strand, within BT1729 at 2128.576 kb on - strand, within BT1729 at 2128.588 kb on - strand, within BT1729 at 2128.621 kb on + strand, within BT1729 at 2128.622 kb on - strand, within BT1729 at 2128.647 kb on - strand, within BT1729 at 2128.649 kb on + strand, within BT1729 at 2128.651 kb on + strand, within BT1729 at 2128.651 kb on + strand, within BT1729 at 2128.682 kb on + strand, within BT1729 at 2128.864 kb on - strand, within BT1729 at 2128.871 kb on + strand, within BT1729 at 2128.881 kb on + strand, within BT1729 at 2128.895 kb on + strand, within BT1729 at 2128.900 kb on + strand, within BT1729 at 2128.901 kb on - strand, within BT1729 at 2128.943 kb on + strand, within BT1729 at 2128.944 kb on - strand, within BT1729 at 2129.051 kb on + strand, within BT1729 at 2129.124 kb on - strand at 2129.145 kb on - strand at 2129.177 kb on + strand at 2129.189 kb on + strand at 2129.194 kb on - strand at 2130.606 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 2,128,214 - BT1729 0.37 -3.2 2,128,223 + BT1729 0.38 +0.0 2,128,224 - BT1729 0.38 +0.0 2,128,224 - BT1729 0.38 -2.2 2,128,228 - BT1729 0.38 -1.0 2,128,245 + BT1729 0.39 +0.0 2,128,246 - BT1729 0.39 -4.6 2,128,246 - BT1729 0.39 -2.3 2,128,296 - BT1729 0.43 -3.1 2,128,301 + BT1729 0.43 +1.3 2,128,360 - BT1729 0.47 -2.4 2,128,443 + BT1729 0.52 -0.3 2,128,575 + BT1729 0.60 -1.0 2,128,576 - BT1729 0.60 -3.6 2,128,588 - BT1729 0.61 -3.8 2,128,621 + BT1729 0.63 +0.0 2,128,622 - BT1729 0.63 +1.6 2,128,647 - BT1729 0.65 -2.3 2,128,649 + BT1729 0.65 -4.3 2,128,651 + BT1729 0.65 -2.3 2,128,651 + BT1729 0.65 +0.0 2,128,682 + BT1729 0.67 -2.6 2,128,864 - BT1729 0.78 +1.6 2,128,871 + BT1729 0.79 -3.2 2,128,881 + BT1729 0.79 +0.0 2,128,895 + BT1729 0.80 -1.5 2,128,900 + BT1729 0.80 -4.1 2,128,901 - BT1729 0.80 +0.6 2,128,943 + BT1729 0.83 -3.6 2,128,944 - BT1729 0.83 -1.3 2,129,051 + BT1729 0.90 -2.6 2,129,124 - -2.4 2,129,145 - -0.8 2,129,177 + -2.8 2,129,189 + +0.3 2,129,194 - -0.5 2,130,606 - -0.5
Or see this region's nucleotide sequence