Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0974 and BT0975 are separated by 1 nucleotides BT0975 and BT0976 are separated by 105 nucleotides BT0976 and BT0977 are separated by 106 nucleotides BT0977 and BT0978 are separated by 82 nucleotides
BT0974: BT0974 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 1,192,047 to 1,192,541
BT0974
BT0975: BT0975 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 1,192,543 to 1,193,310
BT0975
BT0976: BT0976 - putative transport protein (NCBI ptt file), at 1,193,416 to 1,194,669
BT0976
BT0977: BT0977 - hypothetical protein (NCBI ptt file), at 1,194,776 to 1,195,027
BT0977
BT0978: BT0978 - ECF-type sigma factor (NCBI ptt file), at 1,195,110 to 1,195,601
BT0978
Position (kb)
1193
1194
1195 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1192.939 kb on + strand, within BT0975 at 1193.229 kb on + strand, within BT0975 at 1193.313 kb on - strand at 1193.434 kb on + strand at 1193.534 kb on + strand at 1193.534 kb on + strand at 1193.534 kb on + strand at 1193.534 kb on + strand at 1193.577 kb on + strand, within BT0976 at 1193.632 kb on - strand, within BT0976 at 1193.632 kb on - strand, within BT0976 at 1193.637 kb on - strand, within BT0976 at 1193.654 kb on + strand, within BT0976 at 1193.716 kb on + strand, within BT0976 at 1193.810 kb on - strand, within BT0976 at 1193.815 kb on - strand, within BT0976 at 1193.815 kb on - strand, within BT0976 at 1193.921 kb on - strand, within BT0976 at 1193.922 kb on + strand, within BT0976 at 1193.923 kb on - strand, within BT0976 at 1193.928 kb on - strand, within BT0976 at 1193.952 kb on - strand, within BT0976 at 1193.952 kb on - strand, within BT0976 at 1193.952 kb on - strand, within BT0976 at 1193.955 kb on - strand, within BT0976 at 1193.995 kb on - strand, within BT0976 at 1194.099 kb on - strand, within BT0976 at 1194.145 kb on - strand, within BT0976 at 1194.166 kb on + strand, within BT0976 at 1194.218 kb on + strand, within BT0976 at 1194.223 kb on - strand, within BT0976 at 1194.312 kb on + strand, within BT0976 at 1194.413 kb on + strand, within BT0976 at 1194.431 kb on + strand, within BT0976 at 1194.431 kb on + strand, within BT0976 at 1194.432 kb on - strand, within BT0976 at 1194.441 kb on - strand, within BT0976 at 1194.453 kb on - strand, within BT0976 at 1194.552 kb on + strand at 1194.552 kb on + strand at 1194.574 kb on - strand at 1194.574 kb on - strand at 1194.581 kb on - strand at 1194.588 kb on + strand at 1194.590 kb on + strand at 1194.591 kb on - strand at 1194.594 kb on + strand at 1194.597 kb on - strand at 1194.610 kb on - strand at 1194.777 kb on + strand at 1194.806 kb on - strand, within BT0977 at 1194.806 kb on - strand, within BT0977 at 1194.879 kb on - strand, within BT0977 at 1194.908 kb on + strand, within BT0977 at 1194.979 kb on - strand, within BT0977 at 1195.050 kb on - strand at 1195.218 kb on + strand, within BT0978 at 1195.228 kb on - strand, within BT0978 at 1195.228 kb on - strand, within BT0978 at 1195.318 kb on + strand, within BT0978 at 1195.329 kb on + strand, within BT0978 at 1195.345 kb on - strand, within BT0978 at 1195.354 kb on - strand, within BT0978 at 1195.380 kb on - strand, within BT0978 at 1195.381 kb on + strand, within BT0978 at 1195.436 kb on - strand, within BT0978 at 1195.475 kb on + strand, within BT0978 at 1195.480 kb on + strand, within BT0978 at 1195.602 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 1,192,939 + BT0975 0.52 +1.9 1,193,229 + BT0975 0.89 -0.4 1,193,313 - -1.1 1,193,434 + +2.1 1,193,534 + -2.7 1,193,534 + -2.0 1,193,534 + -3.9 1,193,534 + +0.3 1,193,577 + BT0976 0.13 +1.1 1,193,632 - BT0976 0.17 -2.5 1,193,632 - BT0976 0.17 +2.0 1,193,637 - BT0976 0.18 -0.4 1,193,654 + BT0976 0.19 -1.4 1,193,716 + BT0976 0.24 -1.4 1,193,810 - BT0976 0.31 -3.6 1,193,815 - BT0976 0.32 +0.6 1,193,815 - BT0976 0.32 +0.4 1,193,921 - BT0976 0.40 -3.0 1,193,922 + BT0976 0.40 -2.0 1,193,923 - BT0976 0.40 -2.3 1,193,928 - BT0976 0.41 -0.0 1,193,952 - BT0976 0.43 -0.7 1,193,952 - BT0976 0.43 -1.4 1,193,952 - BT0976 0.43 -1.9 1,193,955 - BT0976 0.43 -0.9 1,193,995 - BT0976 0.46 -2.8 1,194,099 - BT0976 0.54 -1.4 1,194,145 - BT0976 0.58 -3.2 1,194,166 + BT0976 0.60 +3.4 1,194,218 + BT0976 0.64 +2.4 1,194,223 - BT0976 0.64 -3.6 1,194,312 + BT0976 0.71 -1.3 1,194,413 + BT0976 0.80 -3.2 1,194,431 + BT0976 0.81 -2.4 1,194,431 + BT0976 0.81 -3.2 1,194,432 - BT0976 0.81 -3.2 1,194,441 - BT0976 0.82 -2.0 1,194,453 - BT0976 0.83 +1.1 1,194,552 + +2.4 1,194,552 + +0.1 1,194,574 - +1.3 1,194,574 - +1.8 1,194,581 - -3.5 1,194,588 + +1.6 1,194,590 + -4.4 1,194,591 - -1.0 1,194,594 + -0.4 1,194,597 - -2.0 1,194,610 - -0.8 1,194,777 + -0.9 1,194,806 - BT0977 0.12 -2.0 1,194,806 - BT0977 0.12 +0.2 1,194,879 - BT0977 0.41 +2.4 1,194,908 + BT0977 0.52 -1.0 1,194,979 - BT0977 0.81 -1.8 1,195,050 - -2.0 1,195,218 + BT0978 0.22 -1.4 1,195,228 - BT0978 0.24 -0.4 1,195,228 - BT0978 0.24 +0.1 1,195,318 + BT0978 0.42 -0.1 1,195,329 + BT0978 0.45 -1.4 1,195,345 - BT0978 0.48 -2.8 1,195,354 - BT0978 0.50 -4.4 1,195,380 - BT0978 0.55 -3.6 1,195,381 + BT0978 0.55 -0.1 1,195,436 - BT0978 0.66 -1.4 1,195,475 + BT0978 0.74 -2.4 1,195,480 + BT0978 0.75 -1.4 1,195,602 - -3.0
Or see this region's nucleotide sequence