Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0976

Experiment: BHIS with 5-Fluorouracil 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0974 and BT0975 are separated by 1 nucleotidesBT0975 and BT0976 are separated by 105 nucleotidesBT0976 and BT0977 are separated by 106 nucleotidesBT0977 and BT0978 are separated by 82 nucleotides BT0974: BT0974 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 1,192,047 to 1,192,541 BT0974 BT0975: BT0975 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 1,192,543 to 1,193,310 BT0975 BT0976: BT0976 - putative transport protein (NCBI ptt file), at 1,193,416 to 1,194,669 BT0976 BT0977: BT0977 - hypothetical protein (NCBI ptt file), at 1,194,776 to 1,195,027 BT0977 BT0978: BT0978 - ECF-type sigma factor (NCBI ptt file), at 1,195,110 to 1,195,601 BT0978 Position (kb) 1193 1194 1195Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1192.939 kb on + strand, within BT0975at 1193.229 kb on + strand, within BT0975at 1193.313 kb on - strandat 1193.434 kb on + strandat 1193.534 kb on + strandat 1193.534 kb on + strandat 1193.534 kb on + strandat 1193.534 kb on + strandat 1193.577 kb on + strand, within BT0976at 1193.632 kb on - strand, within BT0976at 1193.632 kb on - strand, within BT0976at 1193.637 kb on - strand, within BT0976at 1193.654 kb on + strand, within BT0976at 1193.716 kb on + strand, within BT0976at 1193.810 kb on - strand, within BT0976at 1193.815 kb on - strand, within BT0976at 1193.815 kb on - strand, within BT0976at 1193.921 kb on - strand, within BT0976at 1193.922 kb on + strand, within BT0976at 1193.923 kb on - strand, within BT0976at 1193.928 kb on - strand, within BT0976at 1193.952 kb on - strand, within BT0976at 1193.952 kb on - strand, within BT0976at 1193.952 kb on - strand, within BT0976at 1193.955 kb on - strand, within BT0976at 1193.995 kb on - strand, within BT0976at 1194.099 kb on - strand, within BT0976at 1194.145 kb on - strand, within BT0976at 1194.166 kb on + strand, within BT0976at 1194.218 kb on + strand, within BT0976at 1194.223 kb on - strand, within BT0976at 1194.312 kb on + strand, within BT0976at 1194.413 kb on + strand, within BT0976at 1194.431 kb on + strand, within BT0976at 1194.431 kb on + strand, within BT0976at 1194.432 kb on - strand, within BT0976at 1194.441 kb on - strand, within BT0976at 1194.453 kb on - strand, within BT0976at 1194.552 kb on + strandat 1194.552 kb on + strandat 1194.574 kb on - strandat 1194.574 kb on - strandat 1194.581 kb on - strandat 1194.588 kb on + strandat 1194.590 kb on + strandat 1194.591 kb on - strandat 1194.594 kb on + strandat 1194.597 kb on - strandat 1194.610 kb on - strandat 1194.777 kb on + strandat 1194.806 kb on - strand, within BT0977at 1194.806 kb on - strand, within BT0977at 1194.879 kb on - strand, within BT0977at 1194.908 kb on + strand, within BT0977at 1194.979 kb on - strand, within BT0977at 1195.050 kb on - strandat 1195.218 kb on + strand, within BT0978at 1195.228 kb on - strand, within BT0978at 1195.228 kb on - strand, within BT0978at 1195.318 kb on + strand, within BT0978at 1195.329 kb on + strand, within BT0978at 1195.345 kb on - strand, within BT0978at 1195.354 kb on - strand, within BT0978at 1195.380 kb on - strand, within BT0978at 1195.381 kb on + strand, within BT0978at 1195.436 kb on - strand, within BT0978at 1195.475 kb on + strand, within BT0978at 1195.480 kb on + strand, within BT0978at 1195.602 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM
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1,192,939 + BT0975 0.52 +1.9
1,193,229 + BT0975 0.89 -0.4
1,193,313 - -1.1
1,193,434 + +2.1
1,193,534 + -2.7
1,193,534 + -2.0
1,193,534 + -3.9
1,193,534 + +0.3
1,193,577 + BT0976 0.13 +1.1
1,193,632 - BT0976 0.17 -2.5
1,193,632 - BT0976 0.17 +2.0
1,193,637 - BT0976 0.18 -0.4
1,193,654 + BT0976 0.19 -1.4
1,193,716 + BT0976 0.24 -1.4
1,193,810 - BT0976 0.31 -3.6
1,193,815 - BT0976 0.32 +0.6
1,193,815 - BT0976 0.32 +0.4
1,193,921 - BT0976 0.40 -3.0
1,193,922 + BT0976 0.40 -2.0
1,193,923 - BT0976 0.40 -2.3
1,193,928 - BT0976 0.41 -0.0
1,193,952 - BT0976 0.43 -0.7
1,193,952 - BT0976 0.43 -1.4
1,193,952 - BT0976 0.43 -1.9
1,193,955 - BT0976 0.43 -0.9
1,193,995 - BT0976 0.46 -2.8
1,194,099 - BT0976 0.54 -1.4
1,194,145 - BT0976 0.58 -3.2
1,194,166 + BT0976 0.60 +3.4
1,194,218 + BT0976 0.64 +2.4
1,194,223 - BT0976 0.64 -3.6
1,194,312 + BT0976 0.71 -1.3
1,194,413 + BT0976 0.80 -3.2
1,194,431 + BT0976 0.81 -2.4
1,194,431 + BT0976 0.81 -3.2
1,194,432 - BT0976 0.81 -3.2
1,194,441 - BT0976 0.82 -2.0
1,194,453 - BT0976 0.83 +1.1
1,194,552 + +2.4
1,194,552 + +0.1
1,194,574 - +1.3
1,194,574 - +1.8
1,194,581 - -3.5
1,194,588 + +1.6
1,194,590 + -4.4
1,194,591 - -1.0
1,194,594 + -0.4
1,194,597 - -2.0
1,194,610 - -0.8
1,194,777 + -0.9
1,194,806 - BT0977 0.12 -2.0
1,194,806 - BT0977 0.12 +0.2
1,194,879 - BT0977 0.41 +2.4
1,194,908 + BT0977 0.52 -1.0
1,194,979 - BT0977 0.81 -1.8
1,195,050 - -2.0
1,195,218 + BT0978 0.22 -1.4
1,195,228 - BT0978 0.24 -0.4
1,195,228 - BT0978 0.24 +0.1
1,195,318 + BT0978 0.42 -0.1
1,195,329 + BT0978 0.45 -1.4
1,195,345 - BT0978 0.48 -2.8
1,195,354 - BT0978 0.50 -4.4
1,195,380 - BT0978 0.55 -3.6
1,195,381 + BT0978 0.55 -0.1
1,195,436 - BT0978 0.66 -1.4
1,195,475 + BT0978 0.74 -2.4
1,195,480 + BT0978 0.75 -1.4
1,195,602 - -3.0

Or see this region's nucleotide sequence