Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0445 and BT0446 are separated by 656 nucleotides BT0446 and BT0447 overlap by 4 nucleotides
BT0445: BT0445 - endoglucanase E precursor (EGE) (NCBI ptt file), at 540,038 to 541,138
BT0445
BT0446: BT0446 - conserved hypothetical protein (NCBI ptt file), at 541,795 to 543,204
BT0446
BT0447: BT0447 - S-layer related protein precursor, sialic acid-specific 9-O-acetylesterase (NCBI ptt file), at 543,201 to 545,855
BT0447
Position (kb) 541
542
543
544 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 540.811 kb on + strand, within BT0445 at 540.812 kb on - strand, within BT0445 at 540.813 kb on + strand, within BT0445 at 540.814 kb on - strand, within BT0445 at 540.814 kb on - strand, within BT0445 at 540.845 kb on + strand, within BT0445 at 540.847 kb on + strand, within BT0445 at 540.848 kb on - strand, within BT0445 at 540.855 kb on + strand, within BT0445 at 540.856 kb on - strand, within BT0445 at 540.858 kb on + strand, within BT0445 at 540.892 kb on + strand, within BT0445 at 540.893 kb on - strand, within BT0445 at 540.897 kb on + strand, within BT0445 at 540.897 kb on + strand, within BT0445 at 540.939 kb on - strand, within BT0445 at 540.980 kb on + strand, within BT0445 at 540.982 kb on + strand, within BT0445 at 541.072 kb on + strand at 541.153 kb on + strand at 541.155 kb on + strand at 541.411 kb on + strand at 541.455 kb on + strand at 541.500 kb on + strand at 541.545 kb on - strand at 541.611 kb on - strand at 541.626 kb on + strand at 541.673 kb on - strand at 541.747 kb on + strand at 541.779 kb on + strand at 541.784 kb on - strand at 541.822 kb on - strand at 542.050 kb on + strand, within BT0446 at 542.225 kb on - strand, within BT0446 at 542.249 kb on - strand, within BT0446 at 542.292 kb on + strand, within BT0446 at 542.293 kb on - strand, within BT0446 at 542.336 kb on - strand, within BT0446 at 542.364 kb on - strand, within BT0446 at 542.364 kb on - strand, within BT0446 at 542.420 kb on + strand, within BT0446 at 542.501 kb on + strand, within BT0446 at 542.569 kb on + strand, within BT0446 at 542.664 kb on + strand, within BT0446 at 542.744 kb on - strand, within BT0446 at 542.915 kb on - strand, within BT0446 at 542.936 kb on + strand, within BT0446 at 542.938 kb on + strand, within BT0446 at 542.976 kb on + strand, within BT0446 at 542.976 kb on + strand, within BT0446 at 543.038 kb on - strand, within BT0446 at 543.045 kb on + strand, within BT0446 at 543.046 kb on - strand, within BT0446 at 543.046 kb on - strand, within BT0446 at 543.073 kb on - strand at 543.135 kb on + strand at 543.264 kb on - strand at 543.279 kb on + strand at 543.366 kb on - strand at 543.423 kb on + strand at 543.424 kb on - strand at 543.430 kb on + strand at 543.536 kb on - strand, within BT0447 at 543.543 kb on + strand, within BT0447 at 543.544 kb on - strand, within BT0447 at 543.544 kb on - strand, within BT0447 at 543.643 kb on + strand, within BT0447 at 543.644 kb on - strand, within BT0447 at 543.746 kb on + strand, within BT0447 at 543.865 kb on + strand, within BT0447 at 544.036 kb on + strand, within BT0447 at 544.040 kb on + strand, within BT0447 at 544.084 kb on + strand, within BT0447 at 544.116 kb on - strand, within BT0447 at 544.158 kb on + strand, within BT0447 at 544.158 kb on + strand, within BT0447 at 544.158 kb on + strand, within BT0447
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 540,811 + BT0445 0.70 -0.4 540,812 - BT0445 0.70 +1.2 540,813 + BT0445 0.70 -1.2 540,814 - BT0445 0.70 -1.4 540,814 - BT0445 0.70 -0.4 540,845 + BT0445 0.73 +1.2 540,847 + BT0445 0.73 -3.1 540,848 - BT0445 0.74 -2.4 540,855 + BT0445 0.74 -2.4 540,856 - BT0445 0.74 +3.1 540,858 + BT0445 0.74 -0.4 540,892 + BT0445 0.78 -1.0 540,893 - BT0445 0.78 -3.7 540,897 + BT0445 0.78 -1.2 540,897 + BT0445 0.78 -1.6 540,939 - BT0445 0.82 -1.0 540,980 + BT0445 0.86 -0.4 540,982 + BT0445 0.86 -1.7 541,072 + -2.6 541,153 + -0.7 541,155 + -1.7 541,411 + -0.4 541,455 + -1.3 541,500 + -2.1 541,545 - +0.6 541,611 - -3.9 541,626 + -0.2 541,673 - -0.1 541,747 + -0.4 541,779 + -0.3 541,784 - -0.4 541,822 - -1.3 542,050 + BT0446 0.18 +1.2 542,225 - BT0446 0.30 -3.0 542,249 - BT0446 0.32 -2.3 542,292 + BT0446 0.35 +0.6 542,293 - BT0446 0.35 -1.4 542,336 - BT0446 0.38 +0.2 542,364 - BT0446 0.40 -2.0 542,364 - BT0446 0.40 +1.2 542,420 + BT0446 0.44 +0.2 542,501 + BT0446 0.50 -0.7 542,569 + BT0446 0.55 -2.0 542,664 + BT0446 0.62 -2.4 542,744 - BT0446 0.67 +0.6 542,915 - BT0446 0.79 -1.4 542,936 + BT0446 0.81 -4.8 542,938 + BT0446 0.81 -0.4 542,976 + BT0446 0.84 -2.7 542,976 + BT0446 0.84 -2.4 543,038 - BT0446 0.88 -2.2 543,045 + BT0446 0.89 -2.6 543,046 - BT0446 0.89 -2.7 543,046 - BT0446 0.89 +1.2 543,073 - -0.4 543,135 + -1.2 543,264 - -2.0 543,279 + +0.3 543,366 - -3.6 543,423 + +0.4 543,424 - -1.7 543,430 + -0.7 543,536 - BT0447 0.13 +0.9 543,543 + BT0447 0.13 +0.6 543,544 - BT0447 0.13 -1.4 543,544 - BT0447 0.13 +3.4 543,643 + BT0447 0.17 -2.4 543,644 - BT0447 0.17 +1.9 543,746 + BT0447 0.21 -2.4 543,865 + BT0447 0.25 +2.4 544,036 + BT0447 0.31 -3.0 544,040 + BT0447 0.32 -3.1 544,084 + BT0447 0.33 -4.5 544,116 - BT0447 0.34 -0.1 544,158 + BT0447 0.36 -2.1 544,158 + BT0447 0.36 -2.8 544,158 + BT0447 0.36 +0.0
Or see this region's nucleotide sequence