Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0378 and BT0379 are separated by 34 nucleotides BT0379 and BT0380 are separated by 4 nucleotides
BT0378: BT0378 - capsular polysaccharide biosynthesis protein capD (NCBI ptt file), at 464,407 to 466,332
BT0378
BT0379: BT0379 - UDP-glucose 6-dehydrogenase (NCBI ptt file), at 466,367 to 467,683
BT0379
BT0380: BT0380 - nucleotide sugar epimerase (NCBI ptt file), at 467,688 to 468,761
BT0380
Position (kb) 466
467
468 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 465.550 kb on - strand, within BT0378 at 465.550 kb on - strand, within BT0378 at 466.331 kb on - strand at 466.340 kb on - strand at 466.526 kb on + strand, within BT0379 at 466.617 kb on + strand, within BT0379 at 466.627 kb on + strand, within BT0379 at 466.628 kb on - strand, within BT0379 at 466.671 kb on + strand, within BT0379 at 466.726 kb on - strand at 466.726 kb on - strand, within BT0379 at 466.732 kb on + strand, within BT0379 at 466.855 kb on - strand, within BT0379 at 466.856 kb on + strand, within BT0379 at 467.036 kb on - strand, within BT0379 at 467.038 kb on - strand, within BT0379 at 467.068 kb on + strand, within BT0379 at 467.071 kb on + strand, within BT0379 at 467.072 kb on - strand, within BT0379 at 467.097 kb on - strand, within BT0379 at 467.113 kb on + strand, within BT0379 at 467.154 kb on + strand, within BT0379 at 467.361 kb on - strand, within BT0379 at 467.361 kb on - strand, within BT0379 at 467.507 kb on - strand, within BT0379 at 467.697 kb on + strand at 467.697 kb on + strand at 467.697 kb on + strand at 467.828 kb on - strand, within BT0380 at 467.833 kb on - strand, within BT0380 at 468.128 kb on + strand, within BT0380 at 468.144 kb on - strand, within BT0380 at 468.144 kb on - strand, within BT0380 at 468.303 kb on + strand, within BT0380 at 468.326 kb on - strand, within BT0380 at 468.358 kb on - strand, within BT0380 at 468.400 kb on + strand, within BT0380 at 468.474 kb on + strand, within BT0380 at 468.486 kb on - strand, within BT0380 at 468.498 kb on - strand, within BT0380 at 468.645 kb on - strand, within BT0380
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 465,550 - BT0378 0.59 -2.7 465,550 - BT0378 0.59 -2.7 466,331 - -0.3 466,340 - -2.7 466,526 + BT0379 0.12 -3.5 466,617 + BT0379 0.19 +2.2 466,627 + BT0379 0.20 +1.2 466,628 - BT0379 0.20 +2.0 466,671 + BT0379 0.23 +0.1 466,726 - -0.3 466,726 - BT0379 0.27 -0.3 466,732 + BT0379 0.28 +0.2 466,855 - BT0379 0.37 -0.2 466,856 + BT0379 0.37 +0.9 467,036 - BT0379 0.51 -0.9 467,038 - BT0379 0.51 -2.3 467,068 + BT0379 0.53 +2.0 467,071 + BT0379 0.53 +0.9 467,072 - BT0379 0.54 +1.2 467,097 - BT0379 0.55 +1.0 467,113 + BT0379 0.57 -0.3 467,154 + BT0379 0.60 -0.3 467,361 - BT0379 0.75 -2.3 467,361 - BT0379 0.75 -0.6 467,507 - BT0379 0.87 -0.0 467,697 + +0.7 467,697 + -1.9 467,697 + +1.2 467,828 - BT0380 0.13 -1.7 467,833 - BT0380 0.14 +0.8 468,128 + BT0380 0.41 +3.0 468,144 - BT0380 0.42 -3.2 468,144 - BT0380 0.42 +1.2 468,303 + BT0380 0.57 -2.3 468,326 - BT0380 0.59 -1.3 468,358 - BT0380 0.62 -0.0 468,400 + BT0380 0.66 +1.0 468,474 + BT0380 0.73 -2.3 468,486 - BT0380 0.74 -1.7 468,498 - BT0380 0.75 -0.3 468,645 - BT0380 0.89 -1.6
Or see this region's nucleotide sequence