Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0206 and BT0207 are separated by 18 nucleotides BT0207 and BT0208 are separated by 17 nucleotides BT0208 and BT0209 are separated by 17 nucleotides
BT0206: BT0206 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 222,704 to 225,646
BT0206
BT0207: BT0207 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 225,665 to 227,200
BT0207
BT0208: BT0208 - hypothetical protein (NCBI ptt file), at 227,218 to 228,117
BT0208
BT0209: BT0209 - hypothetical protein (NCBI ptt file), at 228,135 to 230,087
BT0209
Position (kb) 225
226
227
228 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 224.789 kb on - strand, within BT0206 at 224.789 kb on - strand, within BT0206 at 224.789 kb on - strand, within BT0206 at 224.789 kb on - strand, within BT0206 at 224.792 kb on - strand, within BT0206 at 224.803 kb on - strand, within BT0206 at 224.832 kb on + strand, within BT0206 at 224.860 kb on + strand, within BT0206 at 224.913 kb on - strand, within BT0206 at 224.986 kb on - strand, within BT0206 at 224.993 kb on + strand, within BT0206 at 224.994 kb on - strand, within BT0206 at 224.997 kb on + strand, within BT0206 at 225.205 kb on + strand, within BT0206 at 225.300 kb on + strand, within BT0206 at 225.303 kb on - strand, within BT0206 at 225.303 kb on - strand, within BT0206 at 225.312 kb on - strand, within BT0206 at 225.554 kb on - strand at 225.588 kb on - strand at 225.651 kb on - strand at 225.688 kb on + strand at 225.780 kb on + strand at 225.780 kb on + strand at 225.781 kb on - strand at 225.781 kb on - strand at 225.859 kb on - strand, within BT0207 at 225.953 kb on - strand, within BT0207 at 225.956 kb on - strand, within BT0207 at 226.192 kb on - strand, within BT0207 at 226.251 kb on - strand, within BT0207 at 226.335 kb on + strand, within BT0207 at 226.336 kb on - strand, within BT0207 at 226.352 kb on + strand, within BT0207 at 226.353 kb on - strand, within BT0207 at 226.365 kb on - strand, within BT0207 at 226.403 kb on - strand, within BT0207 at 226.423 kb on + strand, within BT0207 at 226.430 kb on + strand, within BT0207 at 226.458 kb on - strand, within BT0207 at 226.506 kb on - strand, within BT0207 at 226.511 kb on - strand, within BT0207 at 226.514 kb on + strand, within BT0207 at 226.611 kb on - strand, within BT0207 at 226.611 kb on - strand, within BT0207 at 226.758 kb on - strand, within BT0207 at 226.769 kb on - strand, within BT0207 at 226.786 kb on + strand, within BT0207 at 226.787 kb on - strand, within BT0207 at 226.804 kb on + strand, within BT0207 at 226.811 kb on - strand, within BT0207 at 226.836 kb on - strand, within BT0207 at 227.006 kb on + strand, within BT0207 at 227.009 kb on - strand, within BT0207 at 227.125 kb on - strand at 227.125 kb on - strand at 227.131 kb on - strand at 227.234 kb on + strand at 227.264 kb on + strand at 227.531 kb on + strand, within BT0208 at 227.538 kb on + strand, within BT0208 at 227.620 kb on + strand, within BT0208 at 227.763 kb on - strand, within BT0208 at 227.804 kb on - strand, within BT0208 at 227.915 kb on - strand, within BT0208 at 227.954 kb on - strand, within BT0208 at 227.956 kb on - strand, within BT0208 at 227.995 kb on + strand, within BT0208 at 228.019 kb on - strand, within BT0208 at 228.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 224,789 - BT0206 0.71 -0.3 224,789 - BT0206 0.71 -3.3 224,789 - BT0206 0.71 -0.3 224,789 - BT0206 0.71 -3.9 224,792 - BT0206 0.71 -2.7 224,803 - BT0206 0.71 -2.5 224,832 + BT0206 0.72 -0.3 224,860 + BT0206 0.73 +0.4 224,913 - BT0206 0.75 +0.7 224,986 - BT0206 0.78 -0.9 224,993 + BT0206 0.78 -2.3 224,994 - BT0206 0.78 -0.5 224,997 + BT0206 0.78 -2.3 225,205 + BT0206 0.85 -0.7 225,300 + BT0206 0.88 +1.3 225,303 - BT0206 0.88 +1.7 225,303 - BT0206 0.88 -2.6 225,312 - BT0206 0.89 -2.3 225,554 - -3.1 225,588 - -1.1 225,651 - -3.9 225,688 + +2.0 225,780 + -2.9 225,780 + -0.3 225,781 - -3.5 225,781 - -3.1 225,859 - BT0207 0.13 -1.9 225,953 - BT0207 0.19 -1.9 225,956 - BT0207 0.19 -2.9 226,192 - BT0207 0.34 -1.3 226,251 - BT0207 0.38 -1.9 226,335 + BT0207 0.44 -1.9 226,336 - BT0207 0.44 -2.2 226,352 + BT0207 0.45 -2.6 226,353 - BT0207 0.45 -2.1 226,365 - BT0207 0.46 -0.3 226,403 - BT0207 0.48 -1.5 226,423 + BT0207 0.49 +0.3 226,430 + BT0207 0.50 -1.3 226,458 - BT0207 0.52 +1.7 226,506 - BT0207 0.55 -0.3 226,511 - BT0207 0.55 +0.7 226,514 + BT0207 0.55 -2.3 226,611 - BT0207 0.62 -0.3 226,611 - BT0207 0.62 +1.3 226,758 - BT0207 0.71 +0.7 226,769 - BT0207 0.72 -3.3 226,786 + BT0207 0.73 -1.9 226,787 - BT0207 0.73 -2.2 226,804 + BT0207 0.74 +0.7 226,811 - BT0207 0.75 +1.4 226,836 - BT0207 0.76 -3.0 227,006 + BT0207 0.87 +0.3 227,009 - BT0207 0.88 -1.7 227,125 - -2.6 227,125 - -0.9 227,131 - -0.6 227,234 + +3.8 227,264 + -1.9 227,531 + BT0208 0.35 -0.3 227,538 + BT0208 0.36 -3.9 227,620 + BT0208 0.45 -3.1 227,763 - BT0208 0.61 -0.0 227,804 - BT0208 0.65 -3.3 227,915 - BT0208 0.77 -1.6 227,954 - BT0208 0.82 -1.3 227,956 - BT0208 0.82 -1.3 227,995 + BT0208 0.86 -1.9 228,019 - BT0208 0.89 +1.7 228,035 - -0.3
Or see this region's nucleotide sequence