Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0022 and BT0023 are separated by 266 nucleotides BT0023 and BT0024 are separated by 9 nucleotides BT0024 and BT0025 are separated by 24 nucleotides BT0025 and BT0026 are separated by 261 nucleotides
BT0022: BT0022 - conserved hypothetical protein (NCBI ptt file), at 19,044 to 19,334
BT0022
BT0023: BT0023 - transposase (NCBI ptt file), at 19,601 to 19,966
BT0023
BT0024: BT0024 - transposase (NCBI ptt file), at 19,976 to 20,512
BT0024
BT0025: BT0025 - putative transposase (NCBI ptt file), at 20,537 to 20,674
BT0025
BT0026: BT0026 - putative transposase (NCBI ptt file), at 20,936 to 21,358
BT0026
Position (kb) 19
20
21 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 18.990 kb on - strand at 18.993 kb on + strand at 19.021 kb on + strand at 19.080 kb on - strand, within BT0022 at 19.396 kb on - strand at 19.493 kb on - strand at 19.507 kb on + strand at 19.578 kb on + strand at 19.578 kb on + strand at 19.663 kb on + strand, within BT0023 at 19.688 kb on - strand at 19.692 kb on - strand, within BT0023 at 19.798 kb on + strand, within BT0023 at 19.807 kb on + strand, within BT0023 at 19.816 kb on + strand, within BT0023 at 19.825 kb on + strand, within BT0023 at 19.846 kb on - strand, within BT0023 at 19.949 kb on - strand at 19.953 kb on - strand at 19.956 kb on + strand at 20.010 kb on - strand at 20.059 kb on - strand, within BT0024 at 20.063 kb on - strand, within BT0024 at 20.116 kb on - strand, within BT0024 at 20.162 kb on + strand, within BT0024 at 20.203 kb on - strand, within BT0024 at 20.243 kb on + strand, within BT0024 at 20.273 kb on - strand, within BT0024 at 20.532 kb on - strand at 20.538 kb on + strand at 20.621 kb on - strand, within BT0025 at 20.624 kb on - strand, within BT0025 at 20.699 kb on + strand at 20.701 kb on + strand at 20.701 kb on + strand at 20.740 kb on - strand at 20.835 kb on - strand at 20.998 kb on - strand, within BT0026 at 21.454 kb on - strand at 21.467 kb on - strand at 21.496 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 18,990 - +0.4 18,993 + -4.0 19,021 + -4.7 19,080 - BT0022 0.12 -1.2 19,396 - -2.2 19,493 - +0.8 19,507 + -0.3 19,578 + -1.1 19,578 + +4.2 19,663 + BT0023 0.17 -1.5 19,688 - -1.8 19,692 - BT0023 0.25 -4.4 19,798 + BT0023 0.54 -1.6 19,807 + BT0023 0.56 -1.4 19,816 + BT0023 0.59 +0.8 19,825 + BT0023 0.61 +0.8 19,846 - BT0023 0.67 -0.6 19,949 - -1.6 19,953 - -0.8 19,956 + -0.6 20,010 - +1.6 20,059 - BT0024 0.15 +0.5 20,063 - BT0024 0.16 -2.3 20,116 - BT0024 0.26 +1.0 20,162 + BT0024 0.35 +1.4 20,203 - BT0024 0.42 +2.5 20,243 + BT0024 0.50 +0.2 20,273 - BT0024 0.55 -1.8 20,532 - -2.2 20,538 + +0.5 20,621 - BT0025 0.61 -0.6 20,624 - BT0025 0.63 -0.2 20,699 + -0.0 20,701 + +3.3 20,701 + +1.5 20,740 - -0.2 20,835 - -0.2 20,998 - BT0026 0.15 -3.0 21,454 - -2.2 21,467 - -0.9 21,496 + -2.3
Or see this region's nucleotide sequence