Experiment: MoLS4 with L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhB and escT overlap by 4 nucleotides escT and invX are separated by 6 nucleotides invX and sctV are separated by 321 nucleotides
DVUA0101: flhB - type III secretion protein, hrpY/hrcU family (TIGR), at 137,887 to 138,927
flhB
DVUA0102: escT - type III secretion inner membrane protein (TIGR), at 138,924 to 139,739
escT
DVUA0103: invX - type III secretion protein, HrpO family (TIGR), at 139,746 to 140,006
invX
DVUA0104: sctV - type III secretion inner membrane protein, HrcV family (TIGR), at 140,328 to 142,421
sctV
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-2
-1
0
1 at 138.101 kb on - strand, within flhB at 138.106 kb on - strand, within flhB at 138.148 kb on - strand, within flhB at 138.263 kb on + strand, within flhB at 138.270 kb on + strand, within flhB at 138.271 kb on - strand, within flhB at 138.315 kb on + strand, within flhB at 138.323 kb on - strand, within flhB at 138.341 kb on + strand, within flhB at 138.403 kb on - strand, within flhB at 138.408 kb on + strand, within flhB at 138.458 kb on - strand, within flhB at 138.575 kb on + strand, within flhB at 138.599 kb on - strand, within flhB at 138.622 kb on - strand, within flhB at 138.855 kb on - strand at 138.855 kb on - strand at 139.039 kb on + strand, within escT at 139.049 kb on - strand, within escT at 139.066 kb on + strand, within escT at 139.081 kb on - strand, within escT at 139.234 kb on + strand, within escT at 139.234 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.258 kb on + strand, within escT at 139.266 kb on - strand, within escT at 139.266 kb on - strand, within escT at 139.266 kb on - strand, within escT at 139.266 kb on - strand, within escT at 139.266 kb on - strand, within escT at 139.266 kb on - strand, within escT at 139.276 kb on - strand, within escT at 139.365 kb on - strand, within escT at 139.374 kb on - strand, within escT at 139.415 kb on + strand, within escT at 139.433 kb on - strand, within escT at 139.467 kb on - strand, within escT at 139.483 kb on + strand, within escT at 139.639 kb on - strand, within escT at 139.683 kb on - strand at 139.699 kb on - strand at 139.699 kb on - strand at 139.801 kb on - strand, within invX at 139.848 kb on - strand, within invX at 139.855 kb on - strand, within invX at 139.860 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.895 kb on + strand, within invX at 139.903 kb on - strand, within invX at 139.903 kb on - strand, within invX at 139.905 kb on + strand, within invX at 140.073 kb on - strand at 140.168 kb on - strand at 140.213 kb on + strand at 140.224 kb on + strand at 140.244 kb on + strand at 140.269 kb on + strand at 140.270 kb on - strand at 140.333 kb on + strand at 140.409 kb on - strand at 140.413 kb on + strand at 140.446 kb on - strand at 140.589 kb on - strand, within sctV at 140.605 kb on - strand, within sctV at 140.641 kb on - strand, within sctV at 140.648 kb on + strand, within sctV at 140.693 kb on - strand, within sctV at 140.697 kb on + strand, within sctV at 140.697 kb on + strand, within sctV at 140.707 kb on - strand, within sctV
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Phenylalanine (N) remove 138,101 - flhB DVUA0101 0.21 -0.1 138,106 - flhB DVUA0101 0.21 +0.0 138,148 - flhB DVUA0101 0.25 +0.6 138,263 + flhB DVUA0101 0.36 -0.4 138,270 + flhB DVUA0101 0.37 -0.4 138,271 - flhB DVUA0101 0.37 +0.5 138,315 + flhB DVUA0101 0.41 -0.3 138,323 - flhB DVUA0101 0.42 +0.0 138,341 + flhB DVUA0101 0.44 +0.1 138,403 - flhB DVUA0101 0.50 -0.5 138,408 + flhB DVUA0101 0.50 -0.3 138,458 - flhB DVUA0101 0.55 -0.5 138,575 + flhB DVUA0101 0.66 -0.4 138,599 - flhB DVUA0101 0.68 -0.0 138,622 - flhB DVUA0101 0.71 +0.1 138,855 - +0.3 138,855 - +0.1 139,039 + escT DVUA0102 0.14 -0.1 139,049 - escT DVUA0102 0.15 -0.1 139,066 + escT DVUA0102 0.17 -0.5 139,081 - escT DVUA0102 0.19 -0.4 139,234 + escT DVUA0102 0.38 +0.2 139,234 + escT DVUA0102 0.38 -0.0 139,258 + escT DVUA0102 0.41 -0.3 139,258 + escT DVUA0102 0.41 +0.8 139,258 + escT DVUA0102 0.41 +1.0 139,258 + escT DVUA0102 0.41 +0.4 139,258 + escT DVUA0102 0.41 +0.5 139,258 + escT DVUA0102 0.41 -0.2 139,258 + escT DVUA0102 0.41 -0.4 139,258 + escT DVUA0102 0.41 +0.3 139,266 - escT DVUA0102 0.42 +0.4 139,266 - escT DVUA0102 0.42 +0.6 139,266 - escT DVUA0102 0.42 -0.2 139,266 - escT DVUA0102 0.42 +0.7 139,266 - escT DVUA0102 0.42 +1.7 139,266 - escT DVUA0102 0.42 -0.3 139,276 - escT DVUA0102 0.43 +0.2 139,365 - escT DVUA0102 0.54 -0.3 139,374 - escT DVUA0102 0.55 +0.2 139,415 + escT DVUA0102 0.60 -1.5 139,433 - escT DVUA0102 0.62 +0.3 139,467 - escT DVUA0102 0.67 -0.2 139,483 + escT DVUA0102 0.69 -0.1 139,639 - escT DVUA0102 0.88 +1.6 139,683 - +0.1 139,699 - -0.1 139,699 - +0.7 139,801 - invX DVUA0103 0.21 +0.1 139,848 - invX DVUA0103 0.39 -0.2 139,855 - invX DVUA0103 0.42 +0.2 139,860 + invX DVUA0103 0.44 -0.1 139,895 + invX DVUA0103 0.57 +0.8 139,895 + invX DVUA0103 0.57 +0.2 139,895 + invX DVUA0103 0.57 -0.9 139,895 + invX DVUA0103 0.57 -0.5 139,895 + invX DVUA0103 0.57 -0.1 139,903 - invX DVUA0103 0.60 +0.3 139,903 - invX DVUA0103 0.60 +0.1 139,905 + invX DVUA0103 0.61 -0.0 140,073 - -0.7 140,168 - -0.2 140,213 + +0.0 140,224 + +0.2 140,244 + -2.0 140,269 + -0.4 140,270 - +0.3 140,333 + -0.6 140,409 - -0.7 140,413 + +0.1 140,446 - -0.7 140,589 - sctV DVUA0104 0.12 -0.0 140,605 - sctV DVUA0104 0.13 -0.3 140,641 - sctV DVUA0104 0.15 +0.1 140,648 + sctV DVUA0104 0.15 -1.2 140,693 - sctV DVUA0104 0.17 -0.1 140,697 + sctV DVUA0104 0.18 +0.2 140,697 + sctV DVUA0104 0.18 -0.7 140,707 - sctV DVUA0104 0.18 -0.9
Or see this region's nucleotide sequence