Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01220 and CCNA_01221 are separated by 3 nucleotides CCNA_01221 and CCNA_01222 are separated by 67 nucleotides CCNA_01222 and CCNA_01223 overlap by 11 nucleotides
CCNA_01220: CCNA_01220 - 7-keto-8-aminopelargonate synthetase, at 1,346,517 to 1,347,731
_01220
CCNA_01221: CCNA_01221 - acyl carrier protein, at 1,347,735 to 1,347,995
_01221
CCNA_01222: CCNA_01222 - NADH-ubiquinone oxidoreductase, at 1,348,063 to 1,349,016
_01222
CCNA_01223: CCNA_01223 - acyl-CoA synthetase, at 1,349,006 to 1,350,724
_01223
Position (kb)
1348
1349
1350 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1347.078 kb on + strand, within CCNA_01220 at 1347.108 kb on - strand, within CCNA_01220 at 1347.158 kb on + strand, within CCNA_01220 at 1347.251 kb on - strand, within CCNA_01220 at 1347.291 kb on + strand, within CCNA_01220 at 1347.291 kb on + strand, within CCNA_01220 at 1347.299 kb on - strand, within CCNA_01220 at 1347.314 kb on - strand, within CCNA_01220 at 1347.325 kb on - strand, within CCNA_01220 at 1347.526 kb on + strand, within CCNA_01220 at 1347.554 kb on + strand, within CCNA_01220 at 1347.614 kb on - strand at 1347.627 kb on - strand at 1347.648 kb on - strand at 1347.661 kb on - strand at 1347.685 kb on + strand at 1347.685 kb on + strand at 1347.693 kb on - strand at 1347.693 kb on - strand at 1347.705 kb on + strand at 1347.747 kb on - strand at 1347.754 kb on + strand at 1347.762 kb on - strand, within CCNA_01221 at 1347.821 kb on + strand, within CCNA_01221 at 1347.924 kb on + strand, within CCNA_01221 at 1347.924 kb on + strand, within CCNA_01221 at 1347.924 kb on + strand, within CCNA_01221 at 1347.932 kb on - strand, within CCNA_01221 at 1347.941 kb on + strand, within CCNA_01221 at 1347.949 kb on - strand, within CCNA_01221 at 1347.955 kb on - strand, within CCNA_01221 at 1347.963 kb on + strand, within CCNA_01221 at 1347.970 kb on - strand at 1347.980 kb on + strand at 1347.998 kb on - strand at 1347.998 kb on - strand at 1348.172 kb on + strand, within CCNA_01222 at 1348.193 kb on + strand, within CCNA_01222 at 1348.205 kb on + strand, within CCNA_01222 at 1348.206 kb on + strand, within CCNA_01222 at 1348.206 kb on + strand, within CCNA_01222 at 1348.214 kb on - strand, within CCNA_01222 at 1348.222 kb on + strand, within CCNA_01222 at 1348.230 kb on - strand, within CCNA_01222 at 1348.323 kb on - strand, within CCNA_01222 at 1348.333 kb on - strand, within CCNA_01222 at 1348.333 kb on - strand, within CCNA_01222 at 1348.366 kb on - strand, within CCNA_01222 at 1348.401 kb on + strand, within CCNA_01222 at 1348.459 kb on + strand, within CCNA_01222 at 1348.467 kb on - strand, within CCNA_01222 at 1348.525 kb on - strand, within CCNA_01222 at 1348.525 kb on - strand, within CCNA_01222 at 1348.527 kb on + strand, within CCNA_01222 at 1348.553 kb on - strand, within CCNA_01222 at 1348.553 kb on - strand, within CCNA_01222 at 1348.616 kb on + strand, within CCNA_01222 at 1348.662 kb on + strand, within CCNA_01222 at 1348.677 kb on - strand, within CCNA_01222 at 1348.717 kb on - strand, within CCNA_01222 at 1348.746 kb on + strand, within CCNA_01222 at 1348.755 kb on + strand, within CCNA_01222 at 1348.763 kb on + strand, within CCNA_01222 at 1348.800 kb on - strand, within CCNA_01222 at 1348.820 kb on - strand, within CCNA_01222 at 1348.825 kb on - strand, within CCNA_01222 at 1348.837 kb on - strand, within CCNA_01222 at 1348.867 kb on - strand, within CCNA_01222 at 1348.880 kb on - strand, within CCNA_01222 at 1349.012 kb on - strand at 1349.045 kb on - strand at 1349.073 kb on + strand at 1349.073 kb on + strand at 1349.080 kb on - strand at 1349.081 kb on - strand at 1349.081 kb on - strand at 1349.081 kb on - strand at 1349.081 kb on - strand at 1349.192 kb on + strand, within CCNA_01223 at 1349.205 kb on + strand, within CCNA_01223 at 1349.261 kb on + strand, within CCNA_01223 at 1349.307 kb on + strand, within CCNA_01223 at 1349.333 kb on + strand, within CCNA_01223 at 1349.526 kb on + strand, within CCNA_01223 at 1349.536 kb on + strand, within CCNA_01223 at 1349.538 kb on + strand, within CCNA_01223 at 1349.544 kb on - strand, within CCNA_01223 at 1349.565 kb on - strand, within CCNA_01223 at 1349.599 kb on - strand, within CCNA_01223 at 1349.660 kb on + strand, within CCNA_01223 at 1349.661 kb on + strand, within CCNA_01223 at 1349.667 kb on + strand, within CCNA_01223 at 1349.667 kb on + strand, within CCNA_01223 at 1349.668 kb on - strand, within CCNA_01223 at 1349.675 kb on - strand, within CCNA_01223 at 1349.675 kb on - strand, within CCNA_01223 at 1349.675 kb on - strand, within CCNA_01223 at 1349.675 kb on - strand, within CCNA_01223 at 1349.698 kb on + strand, within CCNA_01223 at 1349.701 kb on - strand, within CCNA_01223 at 1349.701 kb on - strand, within CCNA_01223 at 1349.701 kb on - strand, within CCNA_01223 at 1349.707 kb on + strand, within CCNA_01223 at 1349.710 kb on - strand, within CCNA_01223 at 1349.720 kb on - strand, within CCNA_01223 at 1349.770 kb on - strand, within CCNA_01223 at 1349.770 kb on - strand, within CCNA_01223 at 1349.807 kb on + strand, within CCNA_01223 at 1349.807 kb on + strand, within CCNA_01223 at 1349.812 kb on + strand, within CCNA_01223 at 1349.815 kb on - strand, within CCNA_01223 at 1349.815 kb on - strand, within CCNA_01223 at 1349.815 kb on - strand, within CCNA_01223 at 1349.820 kb on - strand, within CCNA_01223 at 1349.914 kb on + strand, within CCNA_01223 at 1349.916 kb on + strand, within CCNA_01223 at 1349.935 kb on + strand, within CCNA_01223
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 1,347,078 + CCNA_01220 0.46 +0.5 1,347,108 - CCNA_01220 0.49 -2.7 1,347,158 + CCNA_01220 0.53 +0.8 1,347,251 - CCNA_01220 0.60 -1.1 1,347,291 + CCNA_01220 0.64 -1.3 1,347,291 + CCNA_01220 0.64 -1.6 1,347,299 - CCNA_01220 0.64 -0.2 1,347,314 - CCNA_01220 0.66 +0.3 1,347,325 - CCNA_01220 0.67 -1.8 1,347,526 + CCNA_01220 0.83 -0.7 1,347,554 + CCNA_01220 0.85 +2.8 1,347,614 - -1.0 1,347,627 - +0.2 1,347,648 - -2.1 1,347,661 - -0.7 1,347,685 + -1.5 1,347,685 + -0.9 1,347,693 - -2.0 1,347,693 - -1.6 1,347,705 + -0.7 1,347,747 - -1.9 1,347,754 + -1.2 1,347,762 - CCNA_01221 0.10 -0.5 1,347,821 + CCNA_01221 0.33 -0.5 1,347,924 + CCNA_01221 0.72 -2.6 1,347,924 + CCNA_01221 0.72 -2.6 1,347,924 + CCNA_01221 0.72 +0.0 1,347,932 - CCNA_01221 0.75 -0.1 1,347,941 + CCNA_01221 0.79 -2.4 1,347,949 - CCNA_01221 0.82 -0.6 1,347,955 - CCNA_01221 0.84 -1.4 1,347,963 + CCNA_01221 0.87 -0.2 1,347,970 - -0.4 1,347,980 + -2.2 1,347,998 - +3.8 1,347,998 - +0.7 1,348,172 + CCNA_01222 0.11 -2.8 1,348,193 + CCNA_01222 0.14 -1.0 1,348,205 + CCNA_01222 0.15 -0.6 1,348,206 + CCNA_01222 0.15 -1.0 1,348,206 + CCNA_01222 0.15 +0.2 1,348,214 - CCNA_01222 0.16 -0.4 1,348,222 + CCNA_01222 0.17 +0.0 1,348,230 - CCNA_01222 0.18 +0.0 1,348,323 - CCNA_01222 0.27 -1.3 1,348,333 - CCNA_01222 0.28 +1.6 1,348,333 - CCNA_01222 0.28 -0.9 1,348,366 - CCNA_01222 0.32 -1.5 1,348,401 + CCNA_01222 0.35 -0.7 1,348,459 + CCNA_01222 0.42 -1.0 1,348,467 - CCNA_01222 0.42 -0.9 1,348,525 - CCNA_01222 0.48 -0.6 1,348,525 - CCNA_01222 0.48 -1.8 1,348,527 + CCNA_01222 0.49 -1.0 1,348,553 - CCNA_01222 0.51 -0.6 1,348,553 - CCNA_01222 0.51 -1.3 1,348,616 + CCNA_01222 0.58 +0.3 1,348,662 + CCNA_01222 0.63 +1.2 1,348,677 - CCNA_01222 0.64 +0.2 1,348,717 - CCNA_01222 0.69 -2.8 1,348,746 + CCNA_01222 0.72 -0.5 1,348,755 + CCNA_01222 0.73 -2.5 1,348,763 + CCNA_01222 0.73 -0.6 1,348,800 - CCNA_01222 0.77 -0.2 1,348,820 - CCNA_01222 0.79 -1.6 1,348,825 - CCNA_01222 0.80 -1.7 1,348,837 - CCNA_01222 0.81 +0.5 1,348,867 - CCNA_01222 0.84 -1.8 1,348,880 - CCNA_01222 0.86 -0.3 1,349,012 - -1.1 1,349,045 - +2.8 1,349,073 + -0.9 1,349,073 + -1.3 1,349,080 - -2.3 1,349,081 - -0.8 1,349,081 - -3.3 1,349,081 - +0.0 1,349,081 - -2.7 1,349,192 + CCNA_01223 0.11 -1.6 1,349,205 + CCNA_01223 0.12 -1.2 1,349,261 + CCNA_01223 0.15 +1.2 1,349,307 + CCNA_01223 0.18 -0.8 1,349,333 + CCNA_01223 0.19 -1.6 1,349,526 + CCNA_01223 0.30 -2.4 1,349,536 + CCNA_01223 0.31 -2.9 1,349,538 + CCNA_01223 0.31 -1.4 1,349,544 - CCNA_01223 0.31 -1.1 1,349,565 - CCNA_01223 0.33 -1.3 1,349,599 - CCNA_01223 0.34 +0.5 1,349,660 + CCNA_01223 0.38 +2.1 1,349,661 + CCNA_01223 0.38 +0.0 1,349,667 + CCNA_01223 0.38 +0.8 1,349,667 + CCNA_01223 0.38 +1.1 1,349,668 - CCNA_01223 0.39 -2.1 1,349,675 - CCNA_01223 0.39 -0.2 1,349,675 - CCNA_01223 0.39 -1.9 1,349,675 - CCNA_01223 0.39 -0.7 1,349,675 - CCNA_01223 0.39 -1.8 1,349,698 + CCNA_01223 0.40 -0.3 1,349,701 - CCNA_01223 0.40 -1.2 1,349,701 - CCNA_01223 0.40 -1.8 1,349,701 - CCNA_01223 0.40 +2.8 1,349,707 + CCNA_01223 0.41 -1.9 1,349,710 - CCNA_01223 0.41 -0.4 1,349,720 - CCNA_01223 0.42 -0.8 1,349,770 - CCNA_01223 0.44 -1.7 1,349,770 - CCNA_01223 0.44 -3.0 1,349,807 + CCNA_01223 0.47 -0.5 1,349,807 + CCNA_01223 0.47 -2.3 1,349,812 + CCNA_01223 0.47 -0.6 1,349,815 - CCNA_01223 0.47 +0.5 1,349,815 - CCNA_01223 0.47 -0.5 1,349,815 - CCNA_01223 0.47 -1.5 1,349,820 - CCNA_01223 0.47 -1.4 1,349,914 + CCNA_01223 0.53 -3.2 1,349,916 + CCNA_01223 0.53 -0.2 1,349,935 + CCNA_01223 0.54 -2.3
Or see this region's nucleotide sequence