Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01210 and CCNA_01211 are separated by 161 nucleotides CCNA_01211 and CCNA_01212 overlap by 97 nucleotides CCNA_01212 and CCNA_01213 overlap by 1 nucleotides
CCNA_01210: CCNA_01210 - nucleotidyltransferase family protein, at 1,336,864 to 1,337,625
_01210
CCNA_01211: CCNA_01211 - MobA-like NTP transferase domain protein, at 1,337,787 to 1,338,662
_01211
CCNA_01212: CCNA_01212 - dATP pyrophosphohydrolase, at 1,338,566 to 1,339,720
_01212
CCNA_01213: CCNA_01213 - YjgP/YjgQ family membrane permease, at 1,339,720 to 1,340,781
_01213
Position (kb)
1338
1339
1340 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1337.600 kb on - strand at 1337.619 kb on + strand at 1337.650 kb on - strand at 1337.724 kb on + strand at 1337.729 kb on - strand at 1337.734 kb on - strand at 1337.738 kb on + strand at 1338.561 kb on - strand, within CCNA_01211 at 1338.602 kb on - strand at 1338.602 kb on - strand at 1338.602 kb on - strand at 1338.602 kb on - strand at 1338.602 kb on - strand at 1338.602 kb on - strand at 1338.602 kb on - strand at 1338.602 kb on - strand at 1338.602 kb on - strand at 1338.602 kb on - strand at 1338.690 kb on - strand, within CCNA_01212 at 1338.700 kb on + strand, within CCNA_01212 at 1338.752 kb on + strand, within CCNA_01212 at 1338.804 kb on + strand, within CCNA_01212 at 1338.805 kb on - strand, within CCNA_01212 at 1338.810 kb on + strand, within CCNA_01212 at 1338.812 kb on - strand, within CCNA_01212 at 1338.901 kb on + strand, within CCNA_01212 at 1339.017 kb on + strand, within CCNA_01212 at 1339.054 kb on - strand, within CCNA_01212 at 1339.058 kb on + strand, within CCNA_01212 at 1339.058 kb on + strand, within CCNA_01212 at 1339.058 kb on + strand, within CCNA_01212 at 1339.059 kb on + strand, within CCNA_01212 at 1339.066 kb on - strand, within CCNA_01212 at 1339.066 kb on - strand, within CCNA_01212 at 1339.105 kb on - strand, within CCNA_01212 at 1339.110 kb on + strand, within CCNA_01212 at 1339.116 kb on - strand, within CCNA_01212 at 1339.118 kb on - strand, within CCNA_01212 at 1339.190 kb on - strand, within CCNA_01212 at 1339.198 kb on - strand, within CCNA_01212 at 1339.198 kb on - strand, within CCNA_01212 at 1339.198 kb on - strand, within CCNA_01212 at 1339.198 kb on - strand, within CCNA_01212 at 1339.216 kb on - strand, within CCNA_01212 at 1339.232 kb on + strand, within CCNA_01212 at 1339.241 kb on - strand, within CCNA_01212 at 1339.241 kb on - strand, within CCNA_01212 at 1339.333 kb on + strand, within CCNA_01212 at 1339.353 kb on - strand, within CCNA_01212 at 1339.364 kb on + strand, within CCNA_01212 at 1339.394 kb on - strand, within CCNA_01212 at 1339.394 kb on - strand, within CCNA_01212 at 1339.409 kb on - strand, within CCNA_01212 at 1339.457 kb on - strand, within CCNA_01212 at 1339.490 kb on - strand, within CCNA_01212 at 1339.506 kb on - strand, within CCNA_01212 at 1339.506 kb on - strand, within CCNA_01212 at 1339.526 kb on - strand, within CCNA_01212 at 1339.534 kb on - strand, within CCNA_01212 at 1339.644 kb on + strand at 1339.671 kb on + strand at 1339.694 kb on - strand at 1339.717 kb on - strand at 1340.685 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 1,337,600 - -0.8 1,337,619 + +0.3 1,337,650 - +0.6 1,337,724 + +0.3 1,337,729 - +0.4 1,337,734 - -0.2 1,337,738 + +0.4 1,338,561 - CCNA_01211 0.88 -2.6 1,338,602 - -1.7 1,338,602 - -0.9 1,338,602 - -2.7 1,338,602 - -1.4 1,338,602 - -0.9 1,338,602 - -1.2 1,338,602 - +1.8 1,338,602 - -1.0 1,338,602 - -1.0 1,338,602 - -0.1 1,338,690 - CCNA_01212 0.11 -2.0 1,338,700 + CCNA_01212 0.12 -1.8 1,338,752 + CCNA_01212 0.16 -1.3 1,338,804 + CCNA_01212 0.21 +0.8 1,338,805 - CCNA_01212 0.21 -0.5 1,338,810 + CCNA_01212 0.21 -0.7 1,338,812 - CCNA_01212 0.21 -2.9 1,338,901 + CCNA_01212 0.29 -0.6 1,339,017 + CCNA_01212 0.39 -1.9 1,339,054 - CCNA_01212 0.42 -0.9 1,339,058 + CCNA_01212 0.43 -1.5 1,339,058 + CCNA_01212 0.43 -2.2 1,339,058 + CCNA_01212 0.43 -0.2 1,339,059 + CCNA_01212 0.43 -0.6 1,339,066 - CCNA_01212 0.43 -2.0 1,339,066 - CCNA_01212 0.43 -2.5 1,339,105 - CCNA_01212 0.47 -1.0 1,339,110 + CCNA_01212 0.47 +1.0 1,339,116 - CCNA_01212 0.48 -2.6 1,339,118 - CCNA_01212 0.48 -0.2 1,339,190 - CCNA_01212 0.54 -0.3 1,339,198 - CCNA_01212 0.55 -1.0 1,339,198 - CCNA_01212 0.55 -1.9 1,339,198 - CCNA_01212 0.55 -1.5 1,339,198 - CCNA_01212 0.55 -3.0 1,339,216 - CCNA_01212 0.56 +0.3 1,339,232 + CCNA_01212 0.58 +0.0 1,339,241 - CCNA_01212 0.58 -1.7 1,339,241 - CCNA_01212 0.58 -0.3 1,339,333 + CCNA_01212 0.66 -0.6 1,339,353 - CCNA_01212 0.68 -1.3 1,339,364 + CCNA_01212 0.69 -1.3 1,339,394 - CCNA_01212 0.72 -1.6 1,339,394 - CCNA_01212 0.72 -1.9 1,339,409 - CCNA_01212 0.73 -2.7 1,339,457 - CCNA_01212 0.77 +0.7 1,339,490 - CCNA_01212 0.80 -0.7 1,339,506 - CCNA_01212 0.81 -1.5 1,339,506 - CCNA_01212 0.81 -1.8 1,339,526 - CCNA_01212 0.83 -3.3 1,339,534 - CCNA_01212 0.84 -1.3 1,339,644 + +0.1 1,339,671 + +1.8 1,339,694 - -0.4 1,339,717 - +0.1 1,340,685 - -1.8
Or see this region's nucleotide sequence