Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04870 and PGA1_c04880 are separated by 77 nucleotides PGA1_c04880 and PGA1_c04890 are separated by 9 nucleotides PGA1_c04890 and PGA1_c04900 are separated by 77 nucleotides
PGA1_c04870: PGA1_c04870 - tryptophan synthase beta chain, at 478,592 to 479,845
_c04870
PGA1_c04880: PGA1_c04880 - Uncharacterized protein conserved in bacteria, at 479,923 to 480,306
_c04880
PGA1_c04890: PGA1_c04890 - putative beta-lactamase, at 480,316 to 481,479
_c04890
PGA1_c04900: PGA1_c04900 - peptidyl-tRNA hydrolase Pth, at 481,557 to 482,315
_c04900
Position (kb) 480
481
482 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 479.813 kb on - strand at 479.844 kb on + strand at 479.852 kb on - strand at 479.852 kb on - strand at 479.904 kb on + strand at 479.914 kb on + strand at 479.921 kb on + strand at 479.929 kb on - strand at 479.929 kb on - strand at 479.938 kb on - strand at 479.951 kb on - strand at 479.963 kb on - strand, within PGA1_c04880 at 480.033 kb on - strand, within PGA1_c04880 at 480.033 kb on - strand, within PGA1_c04880 at 480.033 kb on - strand, within PGA1_c04880 at 480.038 kb on - strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.073 kb on + strand, within PGA1_c04880 at 480.073 kb on + strand, within PGA1_c04880 at 480.097 kb on + strand, within PGA1_c04880 at 480.119 kb on + strand, within PGA1_c04880 at 480.131 kb on - strand, within PGA1_c04880 at 480.173 kb on + strand, within PGA1_c04880 at 480.180 kb on + strand, within PGA1_c04880 at 480.188 kb on - strand, within PGA1_c04880 at 480.188 kb on - strand, within PGA1_c04880 at 480.188 kb on - strand, within PGA1_c04880 at 480.191 kb on + strand, within PGA1_c04880 at 480.198 kb on - strand, within PGA1_c04880 at 480.198 kb on - strand, within PGA1_c04880 at 480.199 kb on - strand, within PGA1_c04880 at 480.199 kb on - strand, within PGA1_c04880 at 480.199 kb on - strand, within PGA1_c04880 at 480.232 kb on - strand, within PGA1_c04880 at 480.267 kb on + strand, within PGA1_c04880 at 480.270 kb on - strand at 480.282 kb on + strand at 480.290 kb on - strand at 480.295 kb on + strand at 480.308 kb on + strand at 480.313 kb on + strand at 480.323 kb on + strand at 480.392 kb on + strand at 480.397 kb on + strand at 480.400 kb on - strand at 480.405 kb on - strand at 480.426 kb on + strand at 480.438 kb on - strand, within PGA1_c04890 at 480.455 kb on + strand, within PGA1_c04890 at 480.463 kb on - strand, within PGA1_c04890 at 480.516 kb on - strand, within PGA1_c04890 at 480.540 kb on - strand, within PGA1_c04890 at 480.550 kb on + strand, within PGA1_c04890 at 480.558 kb on - strand, within PGA1_c04890 at 480.583 kb on - strand, within PGA1_c04890 at 480.593 kb on + strand, within PGA1_c04890 at 480.661 kb on + strand, within PGA1_c04890 at 480.666 kb on + strand, within PGA1_c04890 at 480.678 kb on + strand, within PGA1_c04890 at 480.681 kb on - strand, within PGA1_c04890 at 480.692 kb on + strand, within PGA1_c04890 at 480.696 kb on + strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.891 kb on + strand, within PGA1_c04890 at 480.898 kb on + strand, within PGA1_c04890 at 480.899 kb on - strand, within PGA1_c04890 at 480.906 kb on - strand, within PGA1_c04890 at 480.934 kb on + strand, within PGA1_c04890 at 480.970 kb on + strand, within PGA1_c04890 at 480.978 kb on - strand, within PGA1_c04890 at 480.978 kb on - strand, within PGA1_c04890 at 481.007 kb on + strand, within PGA1_c04890 at 481.079 kb on + strand, within PGA1_c04890 at 481.142 kb on + strand, within PGA1_c04890 at 481.199 kb on - strand, within PGA1_c04890 at 481.249 kb on + strand, within PGA1_c04890 at 481.249 kb on + strand, within PGA1_c04890 at 481.259 kb on + strand, within PGA1_c04890 at 481.274 kb on - strand, within PGA1_c04890 at 481.274 kb on - strand, within PGA1_c04890 at 481.284 kb on - strand, within PGA1_c04890 at 481.284 kb on - strand, within PGA1_c04890 at 481.363 kb on - strand at 481.376 kb on + strand at 481.404 kb on + strand at 481.411 kb on + strand at 481.411 kb on + strand at 481.419 kb on - strand at 481.424 kb on + strand at 481.479 kb on + strand at 481.497 kb on - strand at 481.497 kb on - strand at 481.497 kb on - strand at 481.497 kb on - strand at 481.498 kb on + strand at 481.506 kb on - strand at 481.506 kb on - strand at 481.508 kb on - strand at 481.514 kb on + strand at 481.514 kb on + strand at 481.514 kb on + strand at 481.514 kb on + strand at 481.514 kb on + strand at 481.514 kb on + strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.588 kb on - strand at 481.611 kb on + strand at 481.614 kb on + strand at 481.614 kb on + strand at 481.619 kb on - strand at 481.619 kb on - strand at 481.622 kb on - strand at 481.629 kb on - strand at 481.629 kb on - strand at 481.666 kb on - strand, within PGA1_c04900 at 481.711 kb on - strand, within PGA1_c04900 at 481.750 kb on + strand, within PGA1_c04900 at 481.763 kb on + strand, within PGA1_c04900 at 482.327 kb on - strand at 482.327 kb on - strand at 482.327 kb on - strand at 482.329 kb on - strand at 482.341 kb on - strand at 482.350 kb on + strand at 482.350 kb on + strand at 482.350 kb on + strand at 482.350 kb on + strand at 482.358 kb on + strand at 482.358 kb on + strand at 482.358 kb on + strand at 482.358 kb on - strand at 482.358 kb on - strand at 482.366 kb on - strand at 482.366 kb on - strand at 482.399 kb on + strand at 482.419 kb on - strand at 482.421 kb on + strand at 482.435 kb on + strand at 482.435 kb on + strand at 482.443 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 479,813 - +0.2 479,844 + -2.2 479,852 - -0.5 479,852 - -0.4 479,904 + -1.2 479,914 + -0.3 479,921 + -0.5 479,929 - -0.6 479,929 - +0.6 479,938 - +0.5 479,951 - -0.3 479,963 - PGA1_c04880 0.10 +0.1 480,033 - PGA1_c04880 0.29 -0.6 480,033 - PGA1_c04880 0.29 -0.7 480,033 - PGA1_c04880 0.29 +0.3 480,038 - PGA1_c04880 0.30 +1.2 480,063 + PGA1_c04880 0.36 -0.8 480,063 + PGA1_c04880 0.36 -0.5 480,063 + PGA1_c04880 0.36 -0.3 480,063 + PGA1_c04880 0.36 -0.8 480,073 + PGA1_c04880 0.39 -0.9 480,073 + PGA1_c04880 0.39 -2.1 480,097 + PGA1_c04880 0.45 -0.4 480,119 + PGA1_c04880 0.51 -0.4 480,131 - PGA1_c04880 0.54 +1.3 480,173 + PGA1_c04880 0.65 +0.1 480,180 + PGA1_c04880 0.67 -1.2 480,188 - PGA1_c04880 0.69 -0.9 480,188 - PGA1_c04880 0.69 -1.1 480,188 - PGA1_c04880 0.69 +0.1 480,191 + PGA1_c04880 0.70 +1.0 480,198 - PGA1_c04880 0.72 +0.3 480,198 - PGA1_c04880 0.72 -1.1 480,199 - PGA1_c04880 0.72 -0.3 480,199 - PGA1_c04880 0.72 -0.8 480,199 - PGA1_c04880 0.72 +0.9 480,232 - PGA1_c04880 0.80 +1.0 480,267 + PGA1_c04880 0.90 +0.3 480,270 - -3.0 480,282 + +0.7 480,290 - -1.1 480,295 + -0.3 480,308 + -0.2 480,313 + +0.6 480,323 + +0.5 480,392 + +1.7 480,397 + +0.8 480,400 - +0.6 480,405 - +0.0 480,426 + +0.3 480,438 - PGA1_c04890 0.10 +1.3 480,455 + PGA1_c04890 0.12 +0.0 480,463 - PGA1_c04890 0.13 -0.0 480,516 - PGA1_c04890 0.17 -0.0 480,540 - PGA1_c04890 0.19 +0.5 480,550 + PGA1_c04890 0.20 +0.6 480,558 - PGA1_c04890 0.21 +0.7 480,583 - PGA1_c04890 0.23 -1.2 480,593 + PGA1_c04890 0.24 -1.4 480,661 + PGA1_c04890 0.30 -1.0 480,666 + PGA1_c04890 0.30 +1.2 480,678 + PGA1_c04890 0.31 -1.3 480,681 - PGA1_c04890 0.31 +0.0 480,692 + PGA1_c04890 0.32 -0.6 480,696 + PGA1_c04890 0.33 +2.4 480,803 - PGA1_c04890 0.42 -1.5 480,803 - PGA1_c04890 0.42 +0.8 480,803 - PGA1_c04890 0.42 +0.6 480,803 - PGA1_c04890 0.42 -2.1 480,803 - PGA1_c04890 0.42 +0.1 480,803 - PGA1_c04890 0.42 +0.0 480,803 - PGA1_c04890 0.42 -0.3 480,803 - PGA1_c04890 0.42 -0.7 480,891 + PGA1_c04890 0.49 +0.7 480,898 + PGA1_c04890 0.50 +0.2 480,899 - PGA1_c04890 0.50 +1.3 480,906 - PGA1_c04890 0.51 -0.0 480,934 + PGA1_c04890 0.53 -0.3 480,970 + PGA1_c04890 0.56 +1.4 480,978 - PGA1_c04890 0.57 +0.2 480,978 - PGA1_c04890 0.57 -1.8 481,007 + PGA1_c04890 0.59 +0.6 481,079 + PGA1_c04890 0.66 +0.3 481,142 + PGA1_c04890 0.71 -0.6 481,199 - PGA1_c04890 0.76 -0.5 481,249 + PGA1_c04890 0.80 -0.0 481,249 + PGA1_c04890 0.80 -0.9 481,259 + PGA1_c04890 0.81 -1.1 481,274 - PGA1_c04890 0.82 -1.1 481,274 - PGA1_c04890 0.82 -0.8 481,284 - PGA1_c04890 0.83 +0.7 481,284 - PGA1_c04890 0.83 +0.5 481,363 - +0.3 481,376 + +0.3 481,404 + +0.6 481,411 + +1.1 481,411 + +1.7 481,419 - -0.9 481,424 + +1.7 481,479 + +0.3 481,497 - +0.4 481,497 - -1.3 481,497 - +0.2 481,497 - -0.1 481,498 + +0.2 481,506 - +1.7 481,506 - -0.8 481,508 - +0.6 481,514 + -0.4 481,514 + +2.5 481,514 + -0.2 481,514 + +1.3 481,514 + -0.2 481,514 + -0.0 481,522 - -1.4 481,522 - -0.1 481,522 - -0.2 481,522 - -0.8 481,522 - +0.8 481,522 - -0.1 481,522 - -0.1 481,522 - +0.2 481,588 - +2.2 481,611 + +1.4 481,614 + +1.1 481,614 + +0.4 481,619 - +0.7 481,619 - -0.3 481,622 - -0.6 481,629 - +1.0 481,629 - +0.7 481,666 - PGA1_c04900 0.14 -0.5 481,711 - PGA1_c04900 0.20 +1.0 481,750 + PGA1_c04900 0.25 -0.5 481,763 + PGA1_c04900 0.27 -0.3 482,327 - +0.7 482,327 - -0.3 482,327 - -0.8 482,329 - -1.1 482,341 - -0.2 482,350 + +0.5 482,350 + -2.0 482,350 + +1.1 482,350 + -0.1 482,358 + -0.3 482,358 + +1.7 482,358 + -0.1 482,358 - -0.4 482,358 - -0.6 482,366 - +0.4 482,366 - -0.9 482,399 + +0.4 482,419 - +1.7 482,421 + -0.1 482,435 + -0.7 482,435 + -2.4 482,443 - +0.9
Or see this region's nucleotide sequence