Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c30450 and PGA1_c30460 are separated by 88 nucleotides PGA1_c30460 and PGA1_c30470 are separated by 10 nucleotides PGA1_c30470 and PGA1_c30480 are separated by 159 nucleotides
PGA1_c30450: PGA1_c30450 - putative DJ-1/PfpI family protein, at 3,180,707 to 3,181,381
_c30450
PGA1_c30460: PGA1_c30460 - D-alanine aminotransferase Dat, at 3,181,470 to 3,182,330
_c30460
PGA1_c30470: PGA1_c30470 - mandelate racemase / muconate lactonizing enzyme, at 3,182,341 to 3,183,306
_c30470
PGA1_c30480: PGA1_c30480 - Uncharacterized conserved protein, at 3,183,466 to 3,184,467
_c30480
Position (kb)
3182
3183
3184 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3181.349 kb on - strand at 3181.375 kb on + strand at 3181.394 kb on + strand at 3181.422 kb on + strand at 3181.427 kb on + strand at 3181.540 kb on + strand at 3181.558 kb on - strand, within PGA1_c30460 at 3181.589 kb on - strand, within PGA1_c30460 at 3181.595 kb on - strand, within PGA1_c30460 at 3181.683 kb on + strand, within PGA1_c30460 at 3181.683 kb on + strand, within PGA1_c30460 at 3181.683 kb on + strand, within PGA1_c30460 at 3181.691 kb on - strand, within PGA1_c30460 at 3181.691 kb on - strand, within PGA1_c30460 at 3181.705 kb on + strand, within PGA1_c30460 at 3181.708 kb on - strand, within PGA1_c30460 at 3181.708 kb on - strand, within PGA1_c30460 at 3181.770 kb on - strand, within PGA1_c30460 at 3181.782 kb on + strand, within PGA1_c30460 at 3181.833 kb on - strand, within PGA1_c30460 at 3181.856 kb on + strand, within PGA1_c30460 at 3181.866 kb on + strand, within PGA1_c30460 at 3181.869 kb on - strand, within PGA1_c30460 at 3181.871 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.887 kb on - strand, within PGA1_c30460 at 3181.887 kb on - strand, within PGA1_c30460 at 3181.887 kb on - strand, within PGA1_c30460 at 3181.935 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.974 kb on - strand, within PGA1_c30460 at 3181.974 kb on - strand, within PGA1_c30460 at 3181.974 kb on - strand, within PGA1_c30460 at 3182.008 kb on + strand, within PGA1_c30460 at 3182.047 kb on + strand, within PGA1_c30460 at 3182.047 kb on + strand, within PGA1_c30460 at 3182.050 kb on - strand, within PGA1_c30460 at 3182.055 kb on - strand, within PGA1_c30460 at 3182.055 kb on - strand, within PGA1_c30460 at 3182.055 kb on - strand, within PGA1_c30460 at 3182.064 kb on + strand, within PGA1_c30460 at 3182.121 kb on - strand, within PGA1_c30460 at 3182.131 kb on + strand, within PGA1_c30460 at 3182.136 kb on + strand, within PGA1_c30460 at 3182.219 kb on - strand, within PGA1_c30460 at 3182.247 kb on - strand at 3182.318 kb on + strand at 3182.339 kb on + strand at 3182.347 kb on + strand at 3182.347 kb on - strand at 3182.407 kb on + strand at 3182.407 kb on + strand at 3182.446 kb on - strand, within PGA1_c30470 at 3182.487 kb on + strand, within PGA1_c30470 at 3182.517 kb on + strand, within PGA1_c30470 at 3182.576 kb on + strand, within PGA1_c30470 at 3182.662 kb on + strand, within PGA1_c30470 at 3182.662 kb on + strand, within PGA1_c30470 at 3182.670 kb on - strand, within PGA1_c30470 at 3182.773 kb on + strand, within PGA1_c30470 at 3182.928 kb on + strand, within PGA1_c30470 at 3183.085 kb on + strand, within PGA1_c30470 at 3183.127 kb on - strand, within PGA1_c30470 at 3183.150 kb on - strand, within PGA1_c30470 at 3183.293 kb on + strand at 3183.306 kb on - strand at 3183.311 kb on - strand at 3183.313 kb on + strand at 3183.323 kb on - strand at 3183.334 kb on - strand at 3183.356 kb on + strand at 3183.364 kb on - strand at 3183.364 kb on - strand at 3183.471 kb on - strand at 3183.496 kb on - strand at 3183.541 kb on + strand at 3183.541 kb on + strand at 3183.541 kb on + strand at 3183.581 kb on + strand, within PGA1_c30480 at 3183.588 kb on - strand, within PGA1_c30480 at 3183.593 kb on - strand, within PGA1_c30480 at 3183.601 kb on - strand, within PGA1_c30480 at 3183.669 kb on + strand, within PGA1_c30480 at 3183.684 kb on + strand, within PGA1_c30480 at 3183.685 kb on - strand, within PGA1_c30480 at 3183.731 kb on + strand, within PGA1_c30480 at 3183.743 kb on - strand, within PGA1_c30480 at 3183.766 kb on - strand, within PGA1_c30480 at 3183.790 kb on + strand, within PGA1_c30480 at 3183.808 kb on + strand, within PGA1_c30480 at 3183.808 kb on + strand, within PGA1_c30480 at 3183.816 kb on - strand, within PGA1_c30480 at 3183.825 kb on + strand, within PGA1_c30480 at 3183.911 kb on - strand, within PGA1_c30480 at 3183.922 kb on + strand, within PGA1_c30480 at 3183.925 kb on + strand, within PGA1_c30480 at 3183.934 kb on + strand, within PGA1_c30480 at 3183.942 kb on - strand, within PGA1_c30480 at 3183.942 kb on - strand, within PGA1_c30480 at 3183.943 kb on - strand, within PGA1_c30480 at 3183.994 kb on + strand, within PGA1_c30480 at 3184.045 kb on - strand, within PGA1_c30480 at 3184.087 kb on + strand, within PGA1_c30480 at 3184.100 kb on - strand, within PGA1_c30480 at 3184.129 kb on + strand, within PGA1_c30480 at 3184.129 kb on + strand, within PGA1_c30480 at 3184.129 kb on + strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.137 kb on - strand, within PGA1_c30480 at 3184.144 kb on + strand, within PGA1_c30480 at 3184.154 kb on + strand, within PGA1_c30480 at 3184.177 kb on - strand, within PGA1_c30480 at 3184.204 kb on + strand, within PGA1_c30480 at 3184.212 kb on - strand, within PGA1_c30480 at 3184.241 kb on - strand, within PGA1_c30480 at 3184.269 kb on - strand, within PGA1_c30480 at 3184.303 kb on - strand, within PGA1_c30480 at 3184.303 kb on - strand, within PGA1_c30480 at 3184.303 kb on - strand, within PGA1_c30480 at 3184.303 kb on - strand, within PGA1_c30480
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 3,181,349 - +1.3 3,181,375 + -0.6 3,181,394 + +0.1 3,181,422 + -1.1 3,181,427 + +0.9 3,181,540 + -1.8 3,181,558 - PGA1_c30460 0.10 -2.2 3,181,589 - PGA1_c30460 0.14 -1.3 3,181,595 - PGA1_c30460 0.15 -2.0 3,181,683 + PGA1_c30460 0.25 -2.1 3,181,683 + PGA1_c30460 0.25 -2.4 3,181,683 + PGA1_c30460 0.25 -3.1 3,181,691 - PGA1_c30460 0.26 -1.3 3,181,691 - PGA1_c30460 0.26 -1.8 3,181,705 + PGA1_c30460 0.27 -2.1 3,181,708 - PGA1_c30460 0.28 -1.7 3,181,708 - PGA1_c30460 0.28 -3.5 3,181,770 - PGA1_c30460 0.35 +0.5 3,181,782 + PGA1_c30460 0.36 -1.1 3,181,833 - PGA1_c30460 0.42 -3.5 3,181,856 + PGA1_c30460 0.45 -2.0 3,181,866 + PGA1_c30460 0.46 -2.0 3,181,869 - PGA1_c30460 0.46 -4.0 3,181,871 + PGA1_c30460 0.47 -3.1 3,181,879 + PGA1_c30460 0.48 -1.7 3,181,879 + PGA1_c30460 0.48 -3.9 3,181,879 + PGA1_c30460 0.48 -3.2 3,181,879 + PGA1_c30460 0.48 -1.6 3,181,879 + PGA1_c30460 0.48 -2.0 3,181,879 + PGA1_c30460 0.48 +0.3 3,181,879 + PGA1_c30460 0.48 -2.1 3,181,887 - PGA1_c30460 0.48 -1.7 3,181,887 - PGA1_c30460 0.48 -2.5 3,181,887 - PGA1_c30460 0.48 -2.1 3,181,935 + PGA1_c30460 0.54 -2.0 3,181,966 + PGA1_c30460 0.58 -1.5 3,181,966 + PGA1_c30460 0.58 -2.7 3,181,966 + PGA1_c30460 0.58 -2.7 3,181,966 + PGA1_c30460 0.58 -1.6 3,181,974 - PGA1_c30460 0.59 -2.3 3,181,974 - PGA1_c30460 0.59 -1.8 3,181,974 - PGA1_c30460 0.59 -3.6 3,182,008 + PGA1_c30460 0.62 -2.2 3,182,047 + PGA1_c30460 0.67 -2.2 3,182,047 + PGA1_c30460 0.67 -3.2 3,182,050 - PGA1_c30460 0.67 -3.0 3,182,055 - PGA1_c30460 0.68 -2.3 3,182,055 - PGA1_c30460 0.68 -1.7 3,182,055 - PGA1_c30460 0.68 -3.3 3,182,064 + PGA1_c30460 0.69 -2.4 3,182,121 - PGA1_c30460 0.76 -1.8 3,182,131 + PGA1_c30460 0.77 -2.5 3,182,136 + PGA1_c30460 0.77 -2.8 3,182,219 - PGA1_c30460 0.87 -0.5 3,182,247 - -3.6 3,182,318 + -2.2 3,182,339 + +0.5 3,182,347 + +0.3 3,182,347 - +1.2 3,182,407 + -1.1 3,182,407 + -2.5 3,182,446 - PGA1_c30470 0.11 -1.6 3,182,487 + PGA1_c30470 0.15 +0.3 3,182,517 + PGA1_c30470 0.18 -2.8 3,182,576 + PGA1_c30470 0.24 -1.7 3,182,662 + PGA1_c30470 0.33 -3.1 3,182,662 + PGA1_c30470 0.33 -2.2 3,182,670 - PGA1_c30470 0.34 -2.5 3,182,773 + PGA1_c30470 0.45 -2.8 3,182,928 + PGA1_c30470 0.61 -2.1 3,183,085 + PGA1_c30470 0.77 -3.6 3,183,127 - PGA1_c30470 0.81 -0.1 3,183,150 - PGA1_c30470 0.84 -2.8 3,183,293 + -1.0 3,183,306 - -0.5 3,183,311 - -2.8 3,183,313 + -1.5 3,183,323 - -2.5 3,183,334 - -0.6 3,183,356 + -1.0 3,183,364 - -1.1 3,183,364 - +0.1 3,183,471 - -1.6 3,183,496 - -4.4 3,183,541 + -3.0 3,183,541 + -1.8 3,183,541 + -2.1 3,183,581 + PGA1_c30480 0.11 -2.2 3,183,588 - PGA1_c30480 0.12 -1.0 3,183,593 - PGA1_c30480 0.13 -2.2 3,183,601 - PGA1_c30480 0.13 -1.5 3,183,669 + PGA1_c30480 0.20 -0.5 3,183,684 + PGA1_c30480 0.22 -0.1 3,183,685 - PGA1_c30480 0.22 -2.5 3,183,731 + PGA1_c30480 0.26 -3.7 3,183,743 - PGA1_c30480 0.28 -1.3 3,183,766 - PGA1_c30480 0.30 -2.0 3,183,790 + PGA1_c30480 0.32 -1.1 3,183,808 + PGA1_c30480 0.34 -0.7 3,183,808 + PGA1_c30480 0.34 -2.1 3,183,816 - PGA1_c30480 0.35 -2.8 3,183,825 + PGA1_c30480 0.36 -1.6 3,183,911 - PGA1_c30480 0.44 -3.8 3,183,922 + PGA1_c30480 0.46 -2.0 3,183,925 + PGA1_c30480 0.46 -3.3 3,183,934 + PGA1_c30480 0.47 -2.9 3,183,942 - PGA1_c30480 0.48 -3.3 3,183,942 - PGA1_c30480 0.48 -0.8 3,183,943 - PGA1_c30480 0.48 -3.4 3,183,994 + PGA1_c30480 0.53 -1.8 3,184,045 - PGA1_c30480 0.58 -2.0 3,184,087 + PGA1_c30480 0.62 -1.1 3,184,100 - PGA1_c30480 0.63 -2.8 3,184,129 + PGA1_c30480 0.66 -2.5 3,184,129 + PGA1_c30480 0.66 -1.4 3,184,129 + PGA1_c30480 0.66 -2.7 3,184,137 - PGA1_c30480 0.67 -1.8 3,184,137 - PGA1_c30480 0.67 +0.4 3,184,137 - PGA1_c30480 0.67 -2.1 3,184,137 - PGA1_c30480 0.67 -3.0 3,184,137 - PGA1_c30480 0.67 -3.5 3,184,137 - PGA1_c30480 0.67 -1.2 3,184,137 - PGA1_c30480 0.67 -2.1 3,184,137 - PGA1_c30480 0.67 -3.2 3,184,144 + PGA1_c30480 0.68 -2.2 3,184,154 + PGA1_c30480 0.69 -1.3 3,184,177 - PGA1_c30480 0.71 -1.0 3,184,204 + PGA1_c30480 0.74 -2.9 3,184,212 - PGA1_c30480 0.74 -3.8 3,184,241 - PGA1_c30480 0.77 -3.8 3,184,269 - PGA1_c30480 0.80 -0.3 3,184,303 - PGA1_c30480 0.84 -1.3 3,184,303 - PGA1_c30480 0.84 +0.3 3,184,303 - PGA1_c30480 0.84 -2.7 3,184,303 - PGA1_c30480 0.84 -2.1
Or see this region's nucleotide sequence