Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02000 and PGA1_c02010 are separated by 12 nucleotides PGA1_c02010 and PGA1_c02020 are separated by 148 nucleotides PGA1_c02020 and PGA1_c02030 are separated by 218 nucleotides PGA1_c02030 and PGA1_c02040 overlap by 4 nucleotides
PGA1_c02000: PGA1_c02000 - glutamate/glutamine/aspartate/asparagine transport system permease protein BztC, at 182,984 to 184,282
_c02000
PGA1_c02010: PGA1_c02010 - glutamate/glutamine/aspartate/asparagine transport ATP-binding protein BztD, at 184,295 to 185,086
_c02010
PGA1_c02020: PGA1_c02020 - Predicted metal-binding protein, at 185,235 to 185,693
_c02020
PGA1_c02030: PGA1_c02030 - phosphoglycerate mutase-like protein, at 185,912 to 186,409
_c02030
PGA1_c02040: PGA1_c02040 - hypothetical protein, at 186,406 to 187,149
_c02040
Position (kb) 185
186 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 184.247 kb on - strand at 184.276 kb on + strand at 184.276 kb on + strand at 184.279 kb on - strand at 184.284 kb on - strand at 184.284 kb on - strand at 184.476 kb on + strand, within PGA1_c02010 at 184.530 kb on - strand, within PGA1_c02010 at 184.542 kb on + strand, within PGA1_c02010 at 184.542 kb on + strand, within PGA1_c02010 at 184.542 kb on + strand, within PGA1_c02010 at 184.542 kb on + strand, within PGA1_c02010 at 184.542 kb on + strand, within PGA1_c02010 at 184.550 kb on - strand, within PGA1_c02010 at 184.555 kb on - strand, within PGA1_c02010 at 184.584 kb on + strand, within PGA1_c02010 at 184.584 kb on + strand, within PGA1_c02010 at 184.592 kb on - strand, within PGA1_c02010 at 184.592 kb on - strand, within PGA1_c02010 at 184.611 kb on + strand, within PGA1_c02010 at 184.620 kb on - strand, within PGA1_c02010 at 184.621 kb on + strand, within PGA1_c02010 at 184.663 kb on + strand, within PGA1_c02010 at 184.694 kb on + strand, within PGA1_c02010 at 184.768 kb on + strand, within PGA1_c02010 at 184.773 kb on - strand, within PGA1_c02010 at 184.773 kb on - strand, within PGA1_c02010 at 184.775 kb on + strand, within PGA1_c02010 at 184.775 kb on + strand, within PGA1_c02010 at 184.778 kb on - strand, within PGA1_c02010 at 184.786 kb on + strand, within PGA1_c02010 at 184.856 kb on - strand, within PGA1_c02010 at 184.912 kb on + strand, within PGA1_c02010 at 184.919 kb on + strand, within PGA1_c02010 at 184.929 kb on + strand, within PGA1_c02010 at 185.082 kb on + strand at 185.090 kb on - strand at 185.215 kb on + strand at 185.253 kb on + strand at 185.285 kb on - strand, within PGA1_c02020 at 185.323 kb on - strand, within PGA1_c02020 at 185.438 kb on + strand, within PGA1_c02020 at 185.438 kb on + strand, within PGA1_c02020 at 185.438 kb on - strand, within PGA1_c02020 at 185.446 kb on - strand, within PGA1_c02020 at 185.537 kb on + strand, within PGA1_c02020 at 185.545 kb on - strand, within PGA1_c02020 at 185.553 kb on - strand, within PGA1_c02020 at 185.688 kb on + strand at 185.717 kb on + strand at 185.717 kb on + strand at 185.725 kb on - strand at 185.736 kb on - strand at 185.911 kb on - strand at 185.913 kb on - strand at 186.010 kb on + strand, within PGA1_c02030 at 186.124 kb on + strand, within PGA1_c02030 at 186.246 kb on - strand, within PGA1_c02030 at 186.353 kb on + strand, within PGA1_c02030 at 186.391 kb on + strand at 186.457 kb on + strand at 186.527 kb on + strand, within PGA1_c02040 at 186.535 kb on - strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.625 kb on + strand, within PGA1_c02040 at 186.651 kb on - strand, within PGA1_c02040 at 186.651 kb on - strand, within PGA1_c02040 at 186.656 kb on - strand, within PGA1_c02040
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 184,247 - +0.6 184,276 + +0.5 184,276 + +0.5 184,279 - +1.5 184,284 - +0.2 184,284 - -0.1 184,476 + PGA1_c02010 0.23 +0.4 184,530 - PGA1_c02010 0.30 +0.6 184,542 + PGA1_c02010 0.31 +2.2 184,542 + PGA1_c02010 0.31 +1.1 184,542 + PGA1_c02010 0.31 +0.4 184,542 + PGA1_c02010 0.31 -0.1 184,542 + PGA1_c02010 0.31 -0.1 184,550 - PGA1_c02010 0.32 +1.7 184,555 - PGA1_c02010 0.33 +1.0 184,584 + PGA1_c02010 0.36 +1.7 184,584 + PGA1_c02010 0.36 -0.8 184,592 - PGA1_c02010 0.38 +0.8 184,592 - PGA1_c02010 0.38 +0.3 184,611 + PGA1_c02010 0.40 +1.5 184,620 - PGA1_c02010 0.41 +1.6 184,621 + PGA1_c02010 0.41 +0.2 184,663 + PGA1_c02010 0.46 +1.5 184,694 + PGA1_c02010 0.50 -0.3 184,768 + PGA1_c02010 0.60 +1.2 184,773 - PGA1_c02010 0.60 -0.3 184,773 - PGA1_c02010 0.60 +1.3 184,775 + PGA1_c02010 0.61 -0.3 184,775 + PGA1_c02010 0.61 +1.9 184,778 - PGA1_c02010 0.61 +1.7 184,786 + PGA1_c02010 0.62 +0.4 184,856 - PGA1_c02010 0.71 +0.4 184,912 + PGA1_c02010 0.78 +1.1 184,919 + PGA1_c02010 0.79 -0.1 184,929 + PGA1_c02010 0.80 -0.4 185,082 + -0.1 185,090 - +1.3 185,215 + -0.4 185,253 + +0.0 185,285 - PGA1_c02020 0.11 -0.2 185,323 - PGA1_c02020 0.19 -0.0 185,438 + PGA1_c02020 0.44 -2.2 185,438 + PGA1_c02020 0.44 -0.8 185,438 - PGA1_c02020 0.44 -0.2 185,446 - PGA1_c02020 0.46 -1.1 185,537 + PGA1_c02020 0.66 -3.9 185,545 - PGA1_c02020 0.68 -0.4 185,553 - PGA1_c02020 0.69 -2.2 185,688 + -0.4 185,717 + -1.5 185,717 + -1.8 185,725 - -1.4 185,736 - -0.3 185,911 - -1.4 185,913 - +0.1 186,010 + PGA1_c02030 0.20 -0.3 186,124 + PGA1_c02030 0.43 -0.4 186,246 - PGA1_c02030 0.67 -1.1 186,353 + PGA1_c02030 0.89 +0.2 186,391 + +0.6 186,457 + +1.1 186,527 + PGA1_c02040 0.16 -0.4 186,535 - PGA1_c02040 0.17 -0.4 186,610 + PGA1_c02040 0.27 +0.2 186,610 + PGA1_c02040 0.27 -0.2 186,610 + PGA1_c02040 0.27 +0.4 186,618 - PGA1_c02040 0.28 +0.4 186,618 - PGA1_c02040 0.28 -0.2 186,618 - PGA1_c02040 0.28 -1.9 186,625 + PGA1_c02040 0.29 -0.6 186,651 - PGA1_c02040 0.33 -0.1 186,651 - PGA1_c02040 0.33 -0.4 186,656 - PGA1_c02040 0.34 -3.2
Or see this region's nucleotide sequence