Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c12500 and PGA1_c12510 are separated by 35 nucleotides PGA1_c12510 and PGA1_c12520 are separated by 274 nucleotides
PGA1_c12500: PGA1_c12500 - Membrane-bound lytic murein transglycosylase B, at 1,298,373 to 1,299,254
_c12500
PGA1_c12510: PGA1_c12510 - transcriptional regulator, AraC family, at 1,299,290 to 1,300,297
_c12510
PGA1_c12520: PGA1_c12520 - ATP-dependent Clp protease proteolytic subunit, at 1,300,572 to 1,301,204
_c12520
Position (kb)
1299
1300
1301 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1298.346 kb on + strand at 1298.354 kb on + strand at 1298.419 kb on + strand at 1298.427 kb on - strand at 1298.428 kb on + strand at 1298.436 kb on - strand at 1298.436 kb on - strand at 1298.477 kb on + strand, within PGA1_c12500 at 1298.477 kb on + strand, within PGA1_c12500 at 1298.485 kb on - strand, within PGA1_c12500 at 1298.485 kb on - strand, within PGA1_c12500 at 1298.553 kb on - strand, within PGA1_c12500 at 1298.593 kb on - strand, within PGA1_c12500 at 1298.600 kb on - strand, within PGA1_c12500 at 1298.616 kb on + strand, within PGA1_c12500 at 1298.616 kb on + strand, within PGA1_c12500 at 1298.621 kb on + strand, within PGA1_c12500 at 1298.621 kb on + strand, within PGA1_c12500 at 1298.639 kb on - strand, within PGA1_c12500 at 1298.675 kb on - strand, within PGA1_c12500 at 1298.705 kb on + strand, within PGA1_c12500 at 1298.728 kb on + strand, within PGA1_c12500 at 1298.743 kb on + strand, within PGA1_c12500 at 1298.760 kb on + strand, within PGA1_c12500 at 1298.852 kb on + strand, within PGA1_c12500 at 1298.863 kb on + strand, within PGA1_c12500 at 1298.957 kb on - strand, within PGA1_c12500 at 1298.969 kb on + strand, within PGA1_c12500 at 1298.977 kb on + strand, within PGA1_c12500 at 1299.033 kb on + strand, within PGA1_c12500 at 1299.038 kb on + strand, within PGA1_c12500 at 1299.045 kb on + strand, within PGA1_c12500 at 1299.046 kb on - strand, within PGA1_c12500 at 1299.101 kb on + strand, within PGA1_c12500 at 1299.109 kb on - strand, within PGA1_c12500 at 1299.151 kb on + strand, within PGA1_c12500 at 1299.173 kb on + strand at 1299.205 kb on + strand at 1299.242 kb on + strand at 1299.243 kb on + strand at 1299.288 kb on + strand at 1299.288 kb on + strand at 1299.288 kb on + strand at 1299.293 kb on + strand at 1299.293 kb on + strand at 1299.301 kb on - strand at 1299.301 kb on - strand at 1299.301 kb on - strand at 1299.366 kb on - strand at 1299.366 kb on - strand at 1299.431 kb on + strand, within PGA1_c12510 at 1299.431 kb on + strand, within PGA1_c12510 at 1299.438 kb on + strand, within PGA1_c12510 at 1299.466 kb on - strand, within PGA1_c12510 at 1299.475 kb on + strand, within PGA1_c12510 at 1299.481 kb on - strand, within PGA1_c12510 at 1299.483 kb on - strand, within PGA1_c12510 at 1299.551 kb on - strand, within PGA1_c12510 at 1299.770 kb on + strand, within PGA1_c12510 at 1299.770 kb on + strand, within PGA1_c12510 at 1299.797 kb on + strand, within PGA1_c12510 at 1299.840 kb on - strand, within PGA1_c12510 at 1299.847 kb on - strand, within PGA1_c12510 at 1299.860 kb on - strand, within PGA1_c12510 at 1299.891 kb on + strand, within PGA1_c12510 at 1299.904 kb on - strand, within PGA1_c12510 at 1299.965 kb on + strand, within PGA1_c12510 at 1299.971 kb on - strand, within PGA1_c12510 at 1300.008 kb on + strand, within PGA1_c12510 at 1300.011 kb on - strand, within PGA1_c12510 at 1300.054 kb on + strand, within PGA1_c12510 at 1300.062 kb on - strand, within PGA1_c12510 at 1300.083 kb on + strand, within PGA1_c12510 at 1300.122 kb on + strand, within PGA1_c12510 at 1300.122 kb on + strand, within PGA1_c12510 at 1300.153 kb on - strand, within PGA1_c12510 at 1300.184 kb on + strand, within PGA1_c12510 at 1300.192 kb on + strand, within PGA1_c12510 at 1300.197 kb on + strand at 1300.306 kb on + strand at 1300.342 kb on + strand at 1300.352 kb on + strand at 1300.355 kb on - strand at 1300.355 kb on - strand at 1300.443 kb on + strand at 1300.443 kb on + strand at 1300.448 kb on + strand at 1300.448 kb on + strand at 1300.453 kb on + strand at 1300.453 kb on + strand at 1300.460 kb on + strand at 1300.492 kb on + strand at 1300.492 kb on + strand at 1300.500 kb on - strand at 1300.512 kb on + strand at 1300.513 kb on + strand at 1301.199 kb on - strand at 1301.203 kb on + strand at 1301.224 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 1,298,346 + +0.7 1,298,354 + -0.8 1,298,419 + +0.2 1,298,427 - +0.9 1,298,428 + +0.1 1,298,436 - -0.6 1,298,436 - +1.4 1,298,477 + PGA1_c12500 0.12 -0.0 1,298,477 + PGA1_c12500 0.12 -1.0 1,298,485 - PGA1_c12500 0.13 +0.3 1,298,485 - PGA1_c12500 0.13 -0.4 1,298,553 - PGA1_c12500 0.20 +0.0 1,298,593 - PGA1_c12500 0.25 -0.4 1,298,600 - PGA1_c12500 0.26 -0.6 1,298,616 + PGA1_c12500 0.28 -1.0 1,298,616 + PGA1_c12500 0.28 +0.4 1,298,621 + PGA1_c12500 0.28 -0.3 1,298,621 + PGA1_c12500 0.28 +0.9 1,298,639 - PGA1_c12500 0.30 +1.0 1,298,675 - PGA1_c12500 0.34 -0.8 1,298,705 + PGA1_c12500 0.38 +0.3 1,298,728 + PGA1_c12500 0.40 -1.9 1,298,743 + PGA1_c12500 0.42 +0.8 1,298,760 + PGA1_c12500 0.44 +1.1 1,298,852 + PGA1_c12500 0.54 +0.3 1,298,863 + PGA1_c12500 0.56 -0.1 1,298,957 - PGA1_c12500 0.66 -1.4 1,298,969 + PGA1_c12500 0.68 +0.6 1,298,977 + PGA1_c12500 0.68 -1.2 1,299,033 + PGA1_c12500 0.75 +0.8 1,299,038 + PGA1_c12500 0.75 -0.7 1,299,045 + PGA1_c12500 0.76 +0.2 1,299,046 - PGA1_c12500 0.76 -2.3 1,299,101 + PGA1_c12500 0.83 -0.8 1,299,109 - PGA1_c12500 0.83 -0.2 1,299,151 + PGA1_c12500 0.88 -0.3 1,299,173 + -0.1 1,299,205 + -0.3 1,299,242 + +1.7 1,299,243 + +0.6 1,299,288 + +0.9 1,299,288 + -0.6 1,299,288 + -0.2 1,299,293 + -0.6 1,299,293 + +0.2 1,299,301 - +0.1 1,299,301 - +0.2 1,299,301 - -2.7 1,299,366 - +0.2 1,299,366 - +0.0 1,299,431 + PGA1_c12510 0.14 +1.0 1,299,431 + PGA1_c12510 0.14 -0.4 1,299,438 + PGA1_c12510 0.15 +0.2 1,299,466 - PGA1_c12510 0.17 -1.1 1,299,475 + PGA1_c12510 0.18 +0.2 1,299,481 - PGA1_c12510 0.19 +1.6 1,299,483 - PGA1_c12510 0.19 -0.4 1,299,551 - PGA1_c12510 0.26 +1.4 1,299,770 + PGA1_c12510 0.48 +0.4 1,299,770 + PGA1_c12510 0.48 +0.7 1,299,797 + PGA1_c12510 0.50 -1.5 1,299,840 - PGA1_c12510 0.55 -1.3 1,299,847 - PGA1_c12510 0.55 +0.5 1,299,860 - PGA1_c12510 0.57 +3.1 1,299,891 + PGA1_c12510 0.60 +0.8 1,299,904 - PGA1_c12510 0.61 -0.4 1,299,965 + PGA1_c12510 0.67 -0.2 1,299,971 - PGA1_c12510 0.68 -0.0 1,300,008 + PGA1_c12510 0.71 +1.1 1,300,011 - PGA1_c12510 0.72 +1.4 1,300,054 + PGA1_c12510 0.76 -0.1 1,300,062 - PGA1_c12510 0.77 +0.5 1,300,083 + PGA1_c12510 0.79 +0.9 1,300,122 + PGA1_c12510 0.83 +1.8 1,300,122 + PGA1_c12510 0.83 -0.4 1,300,153 - PGA1_c12510 0.86 +0.6 1,300,184 + PGA1_c12510 0.89 -0.7 1,300,192 + PGA1_c12510 0.89 +0.8 1,300,197 + -0.1 1,300,306 + -0.7 1,300,342 + -0.2 1,300,352 + +0.1 1,300,355 - -0.8 1,300,355 - +1.2 1,300,443 + +0.6 1,300,443 + -1.1 1,300,448 + -0.4 1,300,448 + -0.9 1,300,453 + +1.4 1,300,453 + +0.5 1,300,460 + +0.3 1,300,492 + -0.4 1,300,492 + -0.2 1,300,500 - -0.3 1,300,512 + -2.0 1,300,513 + -1.2 1,301,199 - -1.8 1,301,203 + +1.4 1,301,224 + -0.2
Or see this region's nucleotide sequence