Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22480 and BWI76_RS22485 are separated by 760 nucleotides BWI76_RS22485 and BWI76_RS22490 are separated by 171 nucleotides BWI76_RS22490 and BWI76_RS22495 overlap by 8 nucleotides
BWI76_RS22480: BWI76_RS22480 - lactaldehyde reductase, at 4,686,792 to 4,687,943
_RS22480
BWI76_RS22485: BWI76_RS22485 - hypothetical protein, at 4,688,704 to 4,689,024
_RS22485
BWI76_RS22490: BWI76_RS22490 - transcriptional regulator, at 4,689,196 to 4,689,936
_RS22490
BWI76_RS22495: BWI76_RS22495 - hypothetical protein, at 4,689,929 to 4,690,417
_RS22495
Position (kb) 4688
4689
4690 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4687.708 kb on - strand, within BWI76_RS22480 at 4687.708 kb on - strand, within BWI76_RS22480 at 4687.708 kb on - strand, within BWI76_RS22480 at 4687.710 kb on + strand, within BWI76_RS22480 at 4687.710 kb on + strand, within BWI76_RS22480 at 4687.710 kb on + strand, within BWI76_RS22480 at 4687.712 kb on + strand, within BWI76_RS22480 at 4687.720 kb on + strand at 4687.721 kb on + strand, within BWI76_RS22480 at 4687.729 kb on - strand, within BWI76_RS22480 at 4687.729 kb on - strand, within BWI76_RS22480 at 4687.729 kb on - strand, within BWI76_RS22480 at 4687.734 kb on + strand, within BWI76_RS22480 at 4687.734 kb on + strand, within BWI76_RS22480 at 4687.742 kb on - strand, within BWI76_RS22480 at 4687.742 kb on - strand, within BWI76_RS22480 at 4687.742 kb on - strand, within BWI76_RS22480 at 4687.759 kb on + strand, within BWI76_RS22480 at 4687.767 kb on - strand, within BWI76_RS22480 at 4687.767 kb on - strand, within BWI76_RS22480 at 4687.777 kb on - strand, within BWI76_RS22480 at 4687.777 kb on - strand, within BWI76_RS22480 at 4687.777 kb on - strand, within BWI76_RS22480 at 4687.777 kb on - strand, within BWI76_RS22480 at 4687.779 kb on + strand, within BWI76_RS22480 at 4687.816 kb on - strand, within BWI76_RS22480 at 4687.816 kb on - strand, within BWI76_RS22480 at 4687.818 kb on + strand, within BWI76_RS22480 at 4687.818 kb on + strand, within BWI76_RS22480 at 4687.826 kb on - strand, within BWI76_RS22480 at 4687.832 kb on - strand at 4687.832 kb on - strand at 4687.834 kb on - strand at 4687.842 kb on - strand at 4687.885 kb on - strand at 4687.888 kb on - strand at 4687.888 kb on - strand at 4687.890 kb on + strand at 4687.914 kb on - strand at 4687.928 kb on - strand at 4687.946 kb on - strand at 4687.947 kb on - strand at 4687.952 kb on + strand at 4687.952 kb on + strand at 4687.954 kb on - strand at 4687.966 kb on - strand at 4687.982 kb on + strand at 4687.992 kb on + strand at 4687.992 kb on + strand at 4687.997 kb on + strand at 4688.000 kb on - strand at 4688.000 kb on - strand at 4688.000 kb on - strand at 4688.000 kb on - strand at 4688.000 kb on - strand at 4688.001 kb on - strand at 4688.057 kb on - strand at 4688.087 kb on + strand at 4688.090 kb on + strand at 4688.092 kb on + strand at 4688.097 kb on + strand at 4688.105 kb on - strand at 4688.105 kb on - strand at 4688.105 kb on - strand at 4688.107 kb on + strand at 4688.110 kb on - strand at 4688.115 kb on - strand at 4688.159 kb on + strand at 4688.159 kb on + strand at 4688.187 kb on - strand at 4688.236 kb on + strand at 4688.288 kb on + strand at 4688.296 kb on - strand at 4688.296 kb on - strand at 4688.304 kb on - strand at 4688.355 kb on + strand at 4688.367 kb on + strand at 4688.370 kb on - strand at 4688.370 kb on - strand at 4688.375 kb on - strand at 4688.375 kb on - strand at 4688.375 kb on - strand at 4688.375 kb on - strand at 4688.375 kb on - strand at 4688.375 kb on - strand at 4688.375 kb on - strand at 4688.375 kb on - strand at 4688.375 kb on - strand at 4688.380 kb on - strand at 4688.381 kb on + strand at 4688.381 kb on + strand at 4688.381 kb on - strand at 4688.381 kb on - strand at 4688.389 kb on - strand at 4688.389 kb on - strand at 4688.389 kb on - strand at 4688.389 kb on - strand at 4688.389 kb on - strand at 4688.395 kb on - strand at 4688.425 kb on - strand at 4688.467 kb on + strand at 4688.467 kb on + strand at 4688.467 kb on + strand at 4688.470 kb on - strand at 4688.472 kb on + strand at 4688.472 kb on + strand at 4688.475 kb on - strand at 4688.475 kb on - strand at 4688.480 kb on - strand at 4688.480 kb on - strand at 4688.480 kb on - strand at 4688.480 kb on - strand at 4688.543 kb on - strand at 4688.547 kb on + strand at 4688.677 kb on + strand at 4688.677 kb on + strand at 4688.677 kb on + strand at 4688.677 kb on + strand at 4688.685 kb on - strand at 4688.685 kb on - strand at 4688.685 kb on - strand at 4688.685 kb on - strand at 4688.685 kb on - strand at 4688.685 kb on - strand at 4688.769 kb on + strand, within BWI76_RS22485 at 4688.856 kb on + strand, within BWI76_RS22485 at 4688.856 kb on + strand, within BWI76_RS22485 at 4688.862 kb on - strand, within BWI76_RS22485 at 4688.862 kb on - strand, within BWI76_RS22485 at 4688.893 kb on + strand, within BWI76_RS22485 at 4688.934 kb on + strand, within BWI76_RS22485 at 4688.934 kb on + strand, within BWI76_RS22485 at 4688.934 kb on + strand, within BWI76_RS22485 at 4688.934 kb on + strand, within BWI76_RS22485 at 4688.934 kb on + strand, within BWI76_RS22485 at 4688.942 kb on - strand, within BWI76_RS22485 at 4688.959 kb on + strand, within BWI76_RS22485 at 4688.959 kb on + strand, within BWI76_RS22485 at 4688.968 kb on - strand, within BWI76_RS22485 at 4689.027 kb on + strand at 4689.034 kb on - strand at 4689.034 kb on - strand at 4689.034 kb on - strand at 4689.048 kb on + strand at 4689.048 kb on + strand at 4689.048 kb on + strand at 4689.053 kb on + strand at 4689.056 kb on - strand at 4689.056 kb on - strand at 4689.056 kb on - strand at 4689.056 kb on - strand at 4689.058 kb on + strand at 4689.067 kb on + strand at 4689.239 kb on + strand at 4689.242 kb on - strand at 4689.244 kb on + strand at 4689.244 kb on + strand at 4689.244 kb on + strand at 4689.244 kb on + strand at 4689.244 kb on + strand at 4689.247 kb on - strand at 4689.249 kb on + strand at 4689.258 kb on + strand at 4689.272 kb on + strand, within BWI76_RS22490 at 4689.272 kb on + strand, within BWI76_RS22490 at 4689.272 kb on + strand, within BWI76_RS22490 at 4689.272 kb on + strand, within BWI76_RS22490 at 4689.297 kb on - strand, within BWI76_RS22490 at 4689.300 kb on + strand, within BWI76_RS22490 at 4689.300 kb on + strand, within BWI76_RS22490 at 4689.300 kb on + strand, within BWI76_RS22490 at 4689.300 kb on + strand, within BWI76_RS22490 at 4689.308 kb on - strand, within BWI76_RS22490 at 4689.308 kb on - strand, within BWI76_RS22490 at 4689.414 kb on + strand, within BWI76_RS22490 at 4689.416 kb on + strand, within BWI76_RS22490 at 4689.416 kb on + strand, within BWI76_RS22490 at 4689.422 kb on - strand, within BWI76_RS22490 at 4689.424 kb on - strand, within BWI76_RS22490 at 4689.424 kb on - strand, within BWI76_RS22490 at 4689.424 kb on - strand, within BWI76_RS22490 at 4689.434 kb on + strand, within BWI76_RS22490 at 4689.442 kb on - strand, within BWI76_RS22490 at 4689.516 kb on - strand, within BWI76_RS22490 at 4689.542 kb on + strand, within BWI76_RS22490 at 4689.542 kb on + strand, within BWI76_RS22490 at 4689.577 kb on - strand, within BWI76_RS22490 at 4689.577 kb on - strand, within BWI76_RS22490 at 4689.577 kb on - strand, within BWI76_RS22490 at 4689.577 kb on - strand, within BWI76_RS22490 at 4689.710 kb on - strand, within BWI76_RS22490 at 4689.715 kb on + strand, within BWI76_RS22490 at 4689.715 kb on + strand, within BWI76_RS22490 at 4689.723 kb on - strand, within BWI76_RS22490 at 4689.723 kb on - strand, within BWI76_RS22490 at 4689.723 kb on - strand, within BWI76_RS22490 at 4689.723 kb on - strand, within BWI76_RS22490 at 4689.723 kb on - strand, within BWI76_RS22490 at 4689.723 kb on - strand, within BWI76_RS22490 at 4689.723 kb on - strand, within BWI76_RS22490 at 4689.723 kb on - strand, within BWI76_RS22490 at 4689.768 kb on + strand, within BWI76_RS22490 at 4689.773 kb on + strand, within BWI76_RS22490 at 4689.776 kb on - strand, within BWI76_RS22490 at 4689.781 kb on - strand, within BWI76_RS22490 at 4689.781 kb on - strand, within BWI76_RS22490 at 4689.781 kb on - strand, within BWI76_RS22490 at 4689.781 kb on - strand, within BWI76_RS22490 at 4689.888 kb on + strand at 4689.897 kb on + strand at 4689.897 kb on + strand at 4689.897 kb on - strand at 4689.897 kb on - strand at 4689.905 kb on - strand at 4689.905 kb on - strand at 4689.962 kb on + strand at 4689.962 kb on + strand at 4689.963 kb on + strand at 4689.965 kb on - strand at 4689.965 kb on - strand at 4689.970 kb on - strand at 4689.983 kb on + strand, within BWI76_RS22495 at 4689.988 kb on + strand, within BWI76_RS22495 at 4689.996 kb on - strand, within BWI76_RS22495 at 4690.001 kb on - strand, within BWI76_RS22495 at 4690.006 kb on - strand, within BWI76_RS22495
Per-strain Table
Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM remove 4,687,708 - BWI76_RS22480 0.80 +0.2 4,687,708 - BWI76_RS22480 0.80 -0.7 4,687,708 - BWI76_RS22480 0.80 +0.6 4,687,710 + BWI76_RS22480 0.80 +1.7 4,687,710 + BWI76_RS22480 0.80 +0.4 4,687,710 + BWI76_RS22480 0.80 +0.4 4,687,712 + BWI76_RS22480 0.80 +0.3 4,687,720 + +0.8 4,687,721 + BWI76_RS22480 0.81 +0.4 4,687,729 - BWI76_RS22480 0.81 +0.1 4,687,729 - BWI76_RS22480 0.81 +0.6 4,687,729 - BWI76_RS22480 0.81 +1.1 4,687,734 + BWI76_RS22480 0.82 +0.8 4,687,734 + BWI76_RS22480 0.82 -0.9 4,687,742 - BWI76_RS22480 0.82 +0.4 4,687,742 - BWI76_RS22480 0.82 -1.3 4,687,742 - BWI76_RS22480 0.82 +0.2 4,687,759 + BWI76_RS22480 0.84 -1.1 4,687,767 - BWI76_RS22480 0.85 +0.1 4,687,767 - BWI76_RS22480 0.85 -0.6 4,687,777 - BWI76_RS22480 0.86 -0.1 4,687,777 - BWI76_RS22480 0.86 -0.8 4,687,777 - BWI76_RS22480 0.86 +0.2 4,687,777 - BWI76_RS22480 0.86 +0.1 4,687,779 + BWI76_RS22480 0.86 -0.3 4,687,816 - BWI76_RS22480 0.89 -1.5 4,687,816 - BWI76_RS22480 0.89 +0.3 4,687,818 + BWI76_RS22480 0.89 -0.6 4,687,818 + BWI76_RS22480 0.89 -0.8 4,687,826 - BWI76_RS22480 0.90 +0.3 4,687,832 - +1.2 4,687,832 - -0.4 4,687,834 - -0.5 4,687,842 - +0.9 4,687,885 - +0.3 4,687,888 - -0.3 4,687,888 - -0.2 4,687,890 + +1.6 4,687,914 - +0.2 4,687,928 - -0.1 4,687,946 - +0.3 4,687,947 - +0.3 4,687,952 + -0.9 4,687,952 + -0.8 4,687,954 - +0.7 4,687,966 - +0.4 4,687,982 + +0.1 4,687,992 + -0.6 4,687,992 + +0.1 4,687,997 + -0.1 4,688,000 - +0.0 4,688,000 - -0.4 4,688,000 - +0.1 4,688,000 - -0.1 4,688,000 - +0.1 4,688,001 - +0.1 4,688,057 - +0.3 4,688,087 + -1.1 4,688,090 + +0.5 4,688,092 + -1.2 4,688,097 + +0.9 4,688,105 - +1.6 4,688,105 - -0.5 4,688,105 - -0.4 4,688,107 + -0.2 4,688,110 - -0.1 4,688,115 - -0.5 4,688,159 + -0.8 4,688,159 + -0.3 4,688,187 - +0.4 4,688,236 + +0.7 4,688,288 + -0.7 4,688,296 - -2.3 4,688,296 - -0.7 4,688,304 - -0.5 4,688,355 + -0.8 4,688,367 + -0.2 4,688,370 - -0.7 4,688,370 - +1.2 4,688,375 - +0.3 4,688,375 - +0.6 4,688,375 - -0.3 4,688,375 - +0.0 4,688,375 - -0.3 4,688,375 - +0.7 4,688,375 - +0.3 4,688,375 - +0.7 4,688,375 - +0.2 4,688,380 - +0.5 4,688,381 + +0.5 4,688,381 + -0.4 4,688,381 - -1.0 4,688,381 - +0.7 4,688,389 - -0.5 4,688,389 - +0.3 4,688,389 - -1.1 4,688,389 - +0.3 4,688,389 - -0.4 4,688,395 - +1.3 4,688,425 - -0.5 4,688,467 + +0.5 4,688,467 + +1.3 4,688,467 + +0.0 4,688,470 - -0.0 4,688,472 + +0.6 4,688,472 + +0.1 4,688,475 - -0.4 4,688,475 - +0.9 4,688,480 - +1.3 4,688,480 - -0.9 4,688,480 - +0.7 4,688,480 - -0.3 4,688,543 - +0.5 4,688,547 + -1.2 4,688,677 + +0.3 4,688,677 + -0.2 4,688,677 + +0.6 4,688,677 + +0.1 4,688,685 - -1.0 4,688,685 - -0.2 4,688,685 - -1.2 4,688,685 - +0.1 4,688,685 - +1.0 4,688,685 - -0.3 4,688,769 + BWI76_RS22485 0.20 -0.7 4,688,856 + BWI76_RS22485 0.47 -1.9 4,688,856 + BWI76_RS22485 0.47 -1.4 4,688,862 - BWI76_RS22485 0.49 -0.1 4,688,862 - BWI76_RS22485 0.49 -1.7 4,688,893 + BWI76_RS22485 0.59 -1.5 4,688,934 + BWI76_RS22485 0.72 -0.1 4,688,934 + BWI76_RS22485 0.72 -2.9 4,688,934 + BWI76_RS22485 0.72 -1.4 4,688,934 + BWI76_RS22485 0.72 -0.4 4,688,934 + BWI76_RS22485 0.72 -1.1 4,688,942 - BWI76_RS22485 0.74 -1.6 4,688,959 + BWI76_RS22485 0.79 -0.1 4,688,959 + BWI76_RS22485 0.79 +0.7 4,688,968 - BWI76_RS22485 0.82 -0.8 4,689,027 + +0.1 4,689,034 - +0.8 4,689,034 - +0.2 4,689,034 - +0.2 4,689,048 + -1.1 4,689,048 + -1.0 4,689,048 + +0.0 4,689,053 + +0.8 4,689,056 - +0.5 4,689,056 - -0.0 4,689,056 - +2.4 4,689,056 - +0.2 4,689,058 + +0.0 4,689,067 + +1.1 4,689,239 + -0.2 4,689,242 - +0.9 4,689,244 + +2.2 4,689,244 + -1.4 4,689,244 + -0.7 4,689,244 + -0.2 4,689,244 + +0.7 4,689,247 - -0.7 4,689,249 + -0.3 4,689,258 + -0.4 4,689,272 + BWI76_RS22490 0.10 -0.5 4,689,272 + BWI76_RS22490 0.10 -0.0 4,689,272 + BWI76_RS22490 0.10 -2.0 4,689,272 + BWI76_RS22490 0.10 +0.1 4,689,297 - BWI76_RS22490 0.14 +1.4 4,689,300 + BWI76_RS22490 0.14 +0.4 4,689,300 + BWI76_RS22490 0.14 -1.1 4,689,300 + BWI76_RS22490 0.14 +1.0 4,689,300 + BWI76_RS22490 0.14 +0.3 4,689,308 - BWI76_RS22490 0.15 -0.4 4,689,308 - BWI76_RS22490 0.15 +0.7 4,689,414 + BWI76_RS22490 0.29 -1.2 4,689,416 + BWI76_RS22490 0.30 +0.2 4,689,416 + BWI76_RS22490 0.30 -1.1 4,689,422 - BWI76_RS22490 0.30 -1.1 4,689,424 - BWI76_RS22490 0.31 -1.9 4,689,424 - BWI76_RS22490 0.31 +0.0 4,689,424 - BWI76_RS22490 0.31 +1.4 4,689,434 + BWI76_RS22490 0.32 +1.2 4,689,442 - BWI76_RS22490 0.33 -1.2 4,689,516 - BWI76_RS22490 0.43 -0.2 4,689,542 + BWI76_RS22490 0.47 -0.2 4,689,542 + BWI76_RS22490 0.47 +0.1 4,689,577 - BWI76_RS22490 0.51 +0.3 4,689,577 - BWI76_RS22490 0.51 +1.2 4,689,577 - BWI76_RS22490 0.51 +0.4 4,689,577 - BWI76_RS22490 0.51 -1.1 4,689,710 - BWI76_RS22490 0.69 +0.1 4,689,715 + BWI76_RS22490 0.70 -0.8 4,689,715 + BWI76_RS22490 0.70 +0.3 4,689,723 - BWI76_RS22490 0.71 +1.4 4,689,723 - BWI76_RS22490 0.71 -0.2 4,689,723 - BWI76_RS22490 0.71 -0.5 4,689,723 - BWI76_RS22490 0.71 -0.7 4,689,723 - BWI76_RS22490 0.71 +1.3 4,689,723 - BWI76_RS22490 0.71 -2.6 4,689,723 - BWI76_RS22490 0.71 +1.5 4,689,723 - BWI76_RS22490 0.71 -1.4 4,689,768 + BWI76_RS22490 0.77 -0.3 4,689,773 + BWI76_RS22490 0.78 +0.6 4,689,776 - BWI76_RS22490 0.78 +0.7 4,689,781 - BWI76_RS22490 0.79 +2.1 4,689,781 - BWI76_RS22490 0.79 +1.0 4,689,781 - BWI76_RS22490 0.79 +1.2 4,689,781 - BWI76_RS22490 0.79 +1.8 4,689,888 + -0.4 4,689,897 + +0.0 4,689,897 + +0.5 4,689,897 - +2.4 4,689,897 - -0.5 4,689,905 - -0.5 4,689,905 - -1.0 4,689,962 + -0.3 4,689,962 + +0.2 4,689,963 + +0.5 4,689,965 - +0.8 4,689,965 - -1.7 4,689,970 - +0.8 4,689,983 + BWI76_RS22495 0.11 +0.3 4,689,988 + BWI76_RS22495 0.12 -1.2 4,689,996 - BWI76_RS22495 0.14 +1.2 4,690,001 - BWI76_RS22495 0.15 -0.6 4,690,006 - BWI76_RS22495 0.16 +0.6
Or see this region's nucleotide sequence