Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06380

Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06375 and BWI76_RS06380 are separated by 60 nucleotidesBWI76_RS06380 and BWI76_RS06385 are separated by 18 nucleotidesBWI76_RS06385 and BWI76_RS06390 are separated by 0 nucleotides BWI76_RS06375: BWI76_RS06375 - oxidoreductase, at 1,339,574 to 1,340,548 _RS06375 BWI76_RS06380: BWI76_RS06380 - 1-deoxy-D-xylulose-5-phosphate synthase, at 1,340,609 to 1,342,471 _RS06380 BWI76_RS06385: BWI76_RS06385 - (2E,6E)-farnesyl diphosphate synthase, at 1,342,490 to 1,343,389 _RS06385 BWI76_RS06390: BWI76_RS06390 - exodeoxyribonuclease VII small subunit, at 1,343,390 to 1,343,632 _RS06390 Position (kb) 1340 1341 1342 1343Strain fitness (log2 ratio) -1 0 1at 1339.611 kb on + strandat 1339.630 kb on + strandat 1339.718 kb on + strand, within BWI76_RS06375at 1339.739 kb on - strand, within BWI76_RS06375at 1340.120 kb on + strand, within BWI76_RS06375at 1340.586 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
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1,339,611 + -0.5
1,339,630 + +0.4
1,339,718 + BWI76_RS06375 0.15 +0.3
1,339,739 - BWI76_RS06375 0.17 -0.3
1,340,120 + BWI76_RS06375 0.56 -0.3
1,340,586 - +0.1

Or see this region's nucleotide sequence