Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04835 and BWI76_RS04840 are separated by 10 nucleotides BWI76_RS04840 and BWI76_RS04845 are separated by 183 nucleotides BWI76_RS04845 and BWI76_RS04850 are separated by 194 nucleotides BWI76_RS04850 and BWI76_RS04855 are separated by 9 nucleotides
BWI76_RS04835: BWI76_RS04835 - type II secretion system protein GspE, at 1,010,005 to 1,011,390
_RS04835
BWI76_RS04840: BWI76_RS04840 - prepilin peptidase-dependent pilin, at 1,011,401 to 1,011,832
_RS04840
BWI76_RS04845: BWI76_RS04845 - nicotinate-nucleotide pyrophosphorylase, at 1,012,016 to 1,012,909
_RS04845
BWI76_RS04850: BWI76_RS04850 - N-acetylmuramoyl-L-alanine amidase, at 1,013,104 to 1,013,655
_RS04850
BWI76_RS04855: BWI76_RS04855 - regulatory protein AmpE, at 1,013,665 to 1,014,519
_RS04855
Position (kb) 1012
1013 Strain fitness (log2 ratio) -2
-1
0
1 at 1011.154 kb on + strand, within BWI76_RS04835 at 1011.270 kb on + strand at 1011.506 kb on - strand, within BWI76_RS04840 at 1011.553 kb on + strand, within BWI76_RS04840 at 1011.670 kb on + strand, within BWI76_RS04840 at 1011.698 kb on + strand, within BWI76_RS04840 at 1011.706 kb on - strand, within BWI76_RS04840 at 1011.808 kb on + strand at 1011.808 kb on + strand at 1012.036 kb on + strand at 1012.100 kb on - strand at 1012.194 kb on - strand, within BWI76_RS04845 at 1012.418 kb on + strand, within BWI76_RS04845 at 1012.426 kb on - strand, within BWI76_RS04845 at 1012.802 kb on + strand, within BWI76_RS04845 at 1012.802 kb on + strand, within BWI76_RS04845 at 1012.810 kb on - strand, within BWI76_RS04845 at 1013.094 kb on - strand at 1013.256 kb on + strand, within BWI76_RS04850 at 1013.368 kb on + strand, within BWI76_RS04850 at 1013.398 kb on + strand, within BWI76_RS04850 at 1013.472 kb on + strand, within BWI76_RS04850 at 1013.514 kb on - strand at 1013.613 kb on - strand at 1013.675 kb on + strand at 1013.681 kb on - strand at 1013.708 kb on + strand at 1013.716 kb on - strand at 1013.716 kb on - strand at 1013.760 kb on - strand, within BWI76_RS04855 at 1013.784 kb on + strand, within BWI76_RS04855 at 1013.844 kb on + strand, within BWI76_RS04855 at 1013.904 kb on + strand, within BWI76_RS04855 at 1013.904 kb on - strand, within BWI76_RS04855
Per-strain Table
Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM remove 1,011,154 + BWI76_RS04835 0.83 -0.5 1,011,270 + -0.3 1,011,506 - BWI76_RS04840 0.24 -0.3 1,011,553 + BWI76_RS04840 0.35 +0.0 1,011,670 + BWI76_RS04840 0.62 +0.2 1,011,698 + BWI76_RS04840 0.69 +0.2 1,011,706 - BWI76_RS04840 0.71 +0.4 1,011,808 + +0.5 1,011,808 + -0.6 1,012,036 + -1.6 1,012,100 - -2.7 1,012,194 - BWI76_RS04845 0.20 -2.7 1,012,418 + BWI76_RS04845 0.45 -0.9 1,012,426 - BWI76_RS04845 0.46 -2.0 1,012,802 + BWI76_RS04845 0.88 -2.0 1,012,802 + BWI76_RS04845 0.88 -1.5 1,012,810 - BWI76_RS04845 0.89 -0.2 1,013,094 - +0.6 1,013,256 + BWI76_RS04850 0.28 -0.6 1,013,368 + BWI76_RS04850 0.48 -0.2 1,013,398 + BWI76_RS04850 0.53 +0.4 1,013,472 + BWI76_RS04850 0.67 -0.3 1,013,514 - -1.1 1,013,613 - -1.0 1,013,675 + -0.2 1,013,681 - +0.8 1,013,708 + -0.0 1,013,716 - -0.3 1,013,716 - -0.3 1,013,760 - BWI76_RS04855 0.11 -0.4 1,013,784 + BWI76_RS04855 0.14 -0.0 1,013,844 + BWI76_RS04855 0.21 -0.1 1,013,904 + BWI76_RS04855 0.28 -0.2 1,013,904 - BWI76_RS04855 0.28 +1.7
Or see this region's nucleotide sequence