Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04305 and BWI76_RS04310 are separated by 327 nucleotides BWI76_RS04310 and BWI76_RS04315 are separated by 505 nucleotides
BWI76_RS04305: BWI76_RS04305 - molecular chaperone DnaJ, at 887,083 to 888,219
_RS04305
BWI76_RS04310: BWI76_RS04310 - hypothetical protein, at 888,547 to 888,726
_RS04310
BWI76_RS04315: BWI76_RS04315 - Na+/H+ antiporter NhaA, at 889,232 to 890,407
_RS04315
Position (kb) 888
889 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 888.082 kb on + strand, within BWI76_RS04305 at 888.108 kb on + strand at 888.108 kb on + strand at 888.128 kb on + strand at 888.128 kb on + strand at 888.128 kb on + strand at 888.128 kb on + strand at 888.128 kb on + strand at 888.128 kb on + strand at 888.136 kb on - strand at 888.145 kb on + strand at 888.146 kb on - strand at 888.148 kb on + strand at 888.153 kb on + strand at 888.153 kb on + strand at 888.158 kb on - strand at 888.166 kb on - strand at 888.205 kb on + strand at 888.207 kb on + strand at 888.375 kb on + strand at 888.395 kb on + strand at 888.395 kb on + strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.403 kb on - strand at 888.438 kb on - strand at 888.438 kb on - strand at 888.547 kb on - strand at 888.547 kb on - strand at 888.547 kb on - strand at 888.574 kb on - strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.642 kb on + strand, within BWI76_RS04310 at 888.645 kb on - strand, within BWI76_RS04310 at 888.647 kb on + strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.650 kb on - strand, within BWI76_RS04310 at 888.689 kb on + strand, within BWI76_RS04310 at 888.689 kb on + strand, within BWI76_RS04310 at 888.707 kb on - strand, within BWI76_RS04310 at 888.707 kb on - strand, within BWI76_RS04310 at 888.734 kb on + strand at 888.757 kb on - strand at 888.792 kb on + strand at 888.810 kb on + strand at 888.811 kb on - strand at 888.818 kb on - strand at 888.841 kb on + strand at 888.841 kb on + strand at 888.849 kb on - strand at 888.849 kb on - strand at 888.849 kb on - strand at 888.854 kb on - strand at 888.854 kb on - strand at 888.856 kb on - strand at 888.950 kb on + strand at 888.950 kb on + strand at 889.089 kb on + strand at 889.303 kb on + strand at 889.315 kb on + strand at 889.330 kb on + strand at 889.370 kb on - strand, within BWI76_RS04315 at 889.466 kb on - strand, within BWI76_RS04315 at 889.508 kb on - strand, within BWI76_RS04315 at 889.508 kb on - strand, within BWI76_RS04315 at 889.603 kb on + strand, within BWI76_RS04315 at 889.655 kb on + strand, within BWI76_RS04315
Per-strain Table
Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM remove 888,082 + BWI76_RS04305 0.88 -3.5 888,108 + -1.2 888,108 + -0.4 888,128 + -1.4 888,128 + +0.3 888,128 + -0.4 888,128 + -0.9 888,128 + -0.3 888,128 + -0.2 888,136 - +0.4 888,145 + -1.1 888,146 - -1.2 888,148 + -2.6 888,153 + -0.3 888,153 + +0.0 888,158 - -1.3 888,166 - -1.6 888,205 + -0.3 888,207 + -0.9 888,375 + -1.0 888,395 + -1.1 888,395 + +0.0 888,403 - +0.2 888,403 - -0.4 888,403 - +1.7 888,403 - -0.2 888,403 - -0.1 888,403 - -0.7 888,438 - +0.4 888,438 - +0.2 888,547 - +1.1 888,547 - +1.1 888,547 - -1.3 888,574 - BWI76_RS04310 0.15 +1.1 888,642 + BWI76_RS04310 0.53 -0.4 888,642 + BWI76_RS04310 0.53 +0.4 888,642 + BWI76_RS04310 0.53 -0.1 888,642 + BWI76_RS04310 0.53 +0.2 888,642 + BWI76_RS04310 0.53 -1.0 888,645 - BWI76_RS04310 0.54 -0.9 888,647 + BWI76_RS04310 0.56 +0.6 888,650 - BWI76_RS04310 0.57 -0.8 888,650 - BWI76_RS04310 0.57 +0.0 888,650 - BWI76_RS04310 0.57 -0.0 888,650 - BWI76_RS04310 0.57 -1.6 888,650 - BWI76_RS04310 0.57 -0.5 888,689 + BWI76_RS04310 0.79 -0.4 888,689 + BWI76_RS04310 0.79 -0.5 888,707 - BWI76_RS04310 0.89 -0.1 888,707 - BWI76_RS04310 0.89 -0.3 888,734 + -0.9 888,757 - +0.1 888,792 + -0.2 888,810 + +0.2 888,811 - -1.4 888,818 - -0.7 888,841 + -0.7 888,841 + -0.5 888,849 - +0.2 888,849 - -0.1 888,849 - -0.1 888,854 - -0.1 888,854 - +0.2 888,856 - -0.1 888,950 + +1.0 888,950 + +0.4 889,089 + +0.7 889,303 + -0.2 889,315 + +0.6 889,330 + -0.8 889,370 - BWI76_RS04315 0.12 -0.2 889,466 - BWI76_RS04315 0.20 -0.9 889,508 - BWI76_RS04315 0.23 +0.2 889,508 - BWI76_RS04315 0.23 +0.0 889,603 + BWI76_RS04315 0.32 +0.3 889,655 + BWI76_RS04315 0.36 -0.2
Or see this region's nucleotide sequence