Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01275 and BWI76_RS01280 are separated by 232 nucleotides BWI76_RS01280 and BWI76_RS01285 are separated by 165 nucleotides BWI76_RS01285 and BWI76_RS01290 are separated by 3 nucleotides
BWI76_RS01275: BWI76_RS01275 - LysR family transcriptional regulator, at 260,795 to 261,748
_RS01275
BWI76_RS01280: BWI76_RS01280 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 261,981 to 264,242
_RS01280
BWI76_RS01285: BWI76_RS01285 - amino acid ABC transporter substrate-binding protein, at 264,408 to 265,097
_RS01285
BWI76_RS01290: BWI76_RS01290 - putative dienelactone hydrolase, at 265,101 to 265,913
_RS01290
Position (kb) 261
262
263
264
265 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 260.996 kb on + strand, within BWI76_RS01275 at 261.005 kb on - strand, within BWI76_RS01275 at 261.109 kb on - strand, within BWI76_RS01275 at 261.114 kb on - strand, within BWI76_RS01275 at 261.435 kb on - strand, within BWI76_RS01275 at 261.625 kb on + strand, within BWI76_RS01275 at 261.633 kb on - strand, within BWI76_RS01275 at 261.663 kb on + strand at 261.699 kb on + strand at 261.904 kb on - strand at 262.023 kb on - strand at 262.089 kb on - strand at 262.183 kb on + strand at 262.201 kb on + strand at 262.226 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.517 kb on - strand, within BWI76_RS01280 at 262.692 kb on - strand, within BWI76_RS01280 at 262.692 kb on - strand, within BWI76_RS01280 at 262.901 kb on + strand, within BWI76_RS01280 at 262.909 kb on - strand, within BWI76_RS01280 at 263.014 kb on + strand, within BWI76_RS01280 at 263.072 kb on - strand, within BWI76_RS01280 at 263.247 kb on - strand, within BWI76_RS01280 at 263.290 kb on + strand, within BWI76_RS01280 at 263.298 kb on - strand, within BWI76_RS01280 at 263.345 kb on + strand, within BWI76_RS01280 at 263.406 kb on + strand, within BWI76_RS01280 at 263.459 kb on - strand, within BWI76_RS01280 at 263.513 kb on - strand, within BWI76_RS01280 at 263.518 kb on - strand, within BWI76_RS01280 at 263.525 kb on - strand, within BWI76_RS01280 at 263.535 kb on - strand, within BWI76_RS01280 at 263.901 kb on + strand, within BWI76_RS01280 at 263.906 kb on + strand, within BWI76_RS01280 at 264.050 kb on + strand at 264.087 kb on - strand at 264.104 kb on + strand at 264.142 kb on + strand at 264.217 kb on - strand at 264.217 kb on - strand at 264.217 kb on - strand at 264.319 kb on - strand at 264.367 kb on - strand at 264.448 kb on + strand at 264.505 kb on + strand, within BWI76_RS01285 at 264.568 kb on - strand, within BWI76_RS01285 at 264.662 kb on - strand, within BWI76_RS01285 at 264.761 kb on - strand, within BWI76_RS01285 at 264.958 kb on + strand, within BWI76_RS01285 at 264.992 kb on + strand, within BWI76_RS01285 at 265.007 kb on - strand, within BWI76_RS01285 at 265.060 kb on - strand at 265.099 kb on - strand at 265.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM remove 260,996 + BWI76_RS01275 0.21 -1.2 261,005 - BWI76_RS01275 0.22 -4.4 261,109 - BWI76_RS01275 0.33 -2.5 261,114 - BWI76_RS01275 0.33 -1.4 261,435 - BWI76_RS01275 0.67 -3.2 261,625 + BWI76_RS01275 0.87 -3.6 261,633 - BWI76_RS01275 0.88 -2.0 261,663 + -2.7 261,699 + -4.8 261,904 - -0.0 262,023 - -0.2 262,089 - -1.1 262,183 + -2.4 262,201 + -0.4 262,226 - BWI76_RS01280 0.11 -1.3 262,504 - BWI76_RS01280 0.23 -2.6 262,504 - BWI76_RS01280 0.23 -1.4 262,504 - BWI76_RS01280 0.23 -0.5 262,504 - BWI76_RS01280 0.23 -0.7 262,504 - BWI76_RS01280 0.23 -0.0 262,504 - BWI76_RS01280 0.23 -2.3 262,517 - BWI76_RS01280 0.24 -1.5 262,692 - BWI76_RS01280 0.31 -2.8 262,692 - BWI76_RS01280 0.31 -2.2 262,901 + BWI76_RS01280 0.41 +1.7 262,909 - BWI76_RS01280 0.41 -1.3 263,014 + BWI76_RS01280 0.46 -1.1 263,072 - BWI76_RS01280 0.48 -1.8 263,247 - BWI76_RS01280 0.56 -1.7 263,290 + BWI76_RS01280 0.58 +0.1 263,298 - BWI76_RS01280 0.58 -1.2 263,345 + BWI76_RS01280 0.60 -0.9 263,406 + BWI76_RS01280 0.63 -1.5 263,459 - BWI76_RS01280 0.65 +1.2 263,513 - BWI76_RS01280 0.68 -0.7 263,518 - BWI76_RS01280 0.68 -1.6 263,525 - BWI76_RS01280 0.68 -0.2 263,535 - BWI76_RS01280 0.69 -2.2 263,901 + BWI76_RS01280 0.85 +0.2 263,906 + BWI76_RS01280 0.85 -0.8 264,050 + -2.1 264,087 - -1.5 264,104 + -1.0 264,142 + -1.1 264,217 - -2.0 264,217 - -0.9 264,217 - -1.5 264,319 - -0.3 264,367 - -0.2 264,448 + -0.4 264,505 + BWI76_RS01285 0.14 +0.1 264,568 - BWI76_RS01285 0.23 +0.1 264,662 - BWI76_RS01285 0.37 +0.7 264,761 - BWI76_RS01285 0.51 -1.6 264,958 + BWI76_RS01285 0.80 +0.2 264,992 + BWI76_RS01285 0.85 -0.9 265,007 - BWI76_RS01285 0.87 +0.1 265,060 - -0.6 265,099 - -0.4 265,132 - -0.4
Or see this region's nucleotide sequence