Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_627

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_626 and Pf1N1B4_627 overlap by 33 nucleotidesPf1N1B4_627 and Pf1N1B4_5174 are separated by 170 nucleotidesPf1N1B4_5174 and Pf1N1B4_628 are separated by 63 nucleotides Pf1N1B4_626 - Quinolinate synthetase (EC 2.5.1.72), at 724,431 to 725,489 _626 Pf1N1B4_627 - hypothetical protein, at 725,457 to 725,573 _627 Pf1N1B4_5174 - tRNA-Lys-TTT, at 725,744 to 725,816 _5174 Pf1N1B4_628 - Queuosine Biosynthesis QueC ATPase, at 725,880 to 726,560 _628 Position (kb) 725 726Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 724.610 kb on + strand, within Pf1N1B4_626at 724.935 kb on - strand, within Pf1N1B4_626at 724.936 kb on + strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 724.937 kb on - strand, within Pf1N1B4_626at 725.072 kb on - strandat 725.072 kb on - strand, within Pf1N1B4_626at 725.072 kb on - strand, within Pf1N1B4_626at 725.084 kb on + strand, within Pf1N1B4_626at 725.084 kb on + strand, within Pf1N1B4_626at 725.085 kb on - strand, within Pf1N1B4_626at 725.085 kb on - strand, within Pf1N1B4_626at 725.341 kb on + strand, within Pf1N1B4_626at 725.341 kb on + strand, within Pf1N1B4_626at 725.342 kb on - strand, within Pf1N1B4_626at 725.397 kb on - strandat 725.404 kb on + strandat 725.405 kb on - strandat 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strand, within Pf1N1B4_627at 725.532 kb on - strandat 725.576 kb on - strandat 725.576 kb on - strandat 725.576 kb on - strandat 725.715 kb on - strandat 725.715 kb on - strandat 725.715 kb on - strandat 725.715 kb on - strandat 725.887 kb on + strandat 725.887 kb on + strandat 725.955 kb on - strand, within Pf1N1B4_628at 726.096 kb on + strand, within Pf1N1B4_628at 726.097 kb on - strand, within Pf1N1B4_628at 726.097 kb on - strand, within Pf1N1B4_628at 726.176 kb on - strand, within Pf1N1B4_628at 726.176 kb on - strand, within Pf1N1B4_628at 726.274 kb on + strand, within Pf1N1B4_628at 726.274 kb on + strand, within Pf1N1B4_628at 726.274 kb on + strand, within Pf1N1B4_628at 726.274 kb on + strand, within Pf1N1B4_628at 726.275 kb on - strand, within Pf1N1B4_628at 726.275 kb on - strand, within Pf1N1B4_628at 726.275 kb on - strand, within Pf1N1B4_628at 726.320 kb on + strand, within Pf1N1B4_628at 726.320 kb on + strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.321 kb on - strand, within Pf1N1B4_628at 726.446 kb on - strand, within Pf1N1B4_628at 726.463 kb on + strand, within Pf1N1B4_628at 726.463 kb on + strand, within Pf1N1B4_628at 726.463 kb on + strand, within Pf1N1B4_628at 726.464 kb on - strand, within Pf1N1B4_628at 726.464 kb on - strand, within Pf1N1B4_628

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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724,610 + Pf1N1B4_626 0.17 -2.2
724,935 - Pf1N1B4_626 0.48 -0.0
724,936 + Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -2.0
724,937 - Pf1N1B4_626 0.48 -2.5
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -3.6
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -1.9
724,937 - Pf1N1B4_626 0.48 -0.0
724,937 - Pf1N1B4_626 0.48 -4.4
725,072 - -0.0
725,072 - Pf1N1B4_626 0.61 -1.6
725,072 - Pf1N1B4_626 0.61 -0.0
725,084 + Pf1N1B4_626 0.62 -0.0
725,084 + Pf1N1B4_626 0.62 -0.0
725,085 - Pf1N1B4_626 0.62 -0.0
725,085 - Pf1N1B4_626 0.62 -1.6
725,341 + Pf1N1B4_626 0.86 -0.0
725,341 + Pf1N1B4_626 0.86 -0.0
725,342 - Pf1N1B4_626 0.86 -0.0
725,397 - -0.0
725,404 + -0.0
725,405 - -1.5
725,532 - Pf1N1B4_627 0.64 -0.0
725,532 - Pf1N1B4_627 0.64 -3.7
725,532 - Pf1N1B4_627 0.64 -3.0
725,532 - Pf1N1B4_627 0.64 -0.0
725,532 - Pf1N1B4_627 0.64 -4.0
725,532 - Pf1N1B4_627 0.64 -0.0
725,532 - Pf1N1B4_627 0.64 -0.0
725,532 - -0.0
725,576 - -2.6
725,576 - -0.0
725,576 - -0.0
725,715 - -0.0
725,715 - -0.0
725,715 - -0.0
725,715 - -0.0
725,887 + -1.0
725,887 + -0.0
725,955 - Pf1N1B4_628 0.11 -2.3
726,096 + Pf1N1B4_628 0.32 -1.7
726,097 - Pf1N1B4_628 0.32 -0.0
726,097 - Pf1N1B4_628 0.32 -0.0
726,176 - Pf1N1B4_628 0.43 -0.0
726,176 - Pf1N1B4_628 0.43 -2.0
726,274 + Pf1N1B4_628 0.58 -0.0
726,274 + Pf1N1B4_628 0.58 -0.0
726,274 + Pf1N1B4_628 0.58 -0.0
726,274 + Pf1N1B4_628 0.58 -0.0
726,275 - Pf1N1B4_628 0.58 -0.0
726,275 - Pf1N1B4_628 0.58 -3.1
726,275 - Pf1N1B4_628 0.58 -2.0
726,320 + Pf1N1B4_628 0.65 -0.0
726,320 + Pf1N1B4_628 0.65 -0.0
726,321 - Pf1N1B4_628 0.65 -0.0
726,321 - Pf1N1B4_628 0.65 -4.8
726,321 - Pf1N1B4_628 0.65 -0.0
726,321 - Pf1N1B4_628 0.65 -2.1
726,321 - Pf1N1B4_628 0.65 -0.0
726,321 - Pf1N1B4_628 0.65 -2.6
726,446 - Pf1N1B4_628 0.83 -0.0
726,463 + Pf1N1B4_628 0.86 -0.0
726,463 + Pf1N1B4_628 0.86 -0.0
726,463 + Pf1N1B4_628 0.86 -0.0
726,464 - Pf1N1B4_628 0.86 -0.0
726,464 - Pf1N1B4_628 0.86 -0.0

Or see this region's nucleotide sequence