Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_616 and Pf1N1B4_617 are separated by 191 nucleotides Pf1N1B4_617 and Pf1N1B4_618 are separated by 12 nucleotides Pf1N1B4_618 and Pf1N1B4_619 are separated by 216 nucleotides
Pf1N1B4_616 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 712,542 to 713,552
_616
Pf1N1B4_617 - Carbamate kinase (EC 2.7.2.2), at 713,744 to 714,673
_617
Pf1N1B4_618 - Acetyl-CoA carboxylase alpha subunit, at 714,686 to 715,048
_618
Pf1N1B4_619 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 715,265 to 716,773
_619
Position (kb) 713
714
715 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 713.550 kb on + strand at 713.575 kb on + strand at 713.576 kb on - strand at 713.744 kb on - strand at 713.933 kb on + strand, within Pf1N1B4_617 at 713.996 kb on + strand at 714.126 kb on - strand, within Pf1N1B4_617 at 714.126 kb on - strand at 714.315 kb on - strand, within Pf1N1B4_617 at 714.315 kb on - strand, within Pf1N1B4_617 at 714.660 kb on + strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.752 kb on + strand at 714.752 kb on + strand, within Pf1N1B4_618 at 714.752 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand at 714.770 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.807 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.920 kb on + strand, within Pf1N1B4_618 at 714.975 kb on + strand, within Pf1N1B4_618 at 715.073 kb on + strand at 715.227 kb on + strand at 715.227 kb on + strand at 715.228 kb on - strand at 715.228 kb on - strand at 715.379 kb on + strand at 715.380 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 712,770 + Pf1N1B4_616 0.23 -2.3 712,770 + Pf1N1B4_616 0.23 -0.0 712,771 - Pf1N1B4_616 0.23 -2.5 712,771 - Pf1N1B4_616 0.23 -0.0 713,550 + -2.7 713,575 + -0.0 713,576 - -2.8 713,744 - -0.0 713,933 + Pf1N1B4_617 0.20 -3.5 713,996 + -2.6 714,126 - Pf1N1B4_617 0.41 -0.0 714,126 - -0.0 714,315 - Pf1N1B4_617 0.61 -0.0 714,315 - Pf1N1B4_617 0.61 -3.3 714,660 + -2.0 714,661 - -0.0 714,661 - -0.0 714,661 - -0.0 714,661 - -0.0 714,665 + -0.0 714,665 + -0.0 714,665 + -0.0 714,665 + -0.0 714,665 + -3.3 714,665 + -0.0 714,665 + -1.7 714,665 + -0.0 714,665 + -0.0 714,665 + -5.2 714,665 + -2.7 714,665 + -0.0 714,665 + -3.1 714,665 + -1.3 714,665 + -1.8 714,665 + -0.0 714,665 + -0.0 714,666 - -0.0 714,666 - -0.0 714,666 - -3.2 714,666 - -0.0 714,666 - -0.0 714,666 - -0.0 714,666 - -2.5 714,666 - -0.0 714,666 - -2.2 714,671 + -3.1 714,671 + -0.0 714,671 + -0.0 714,672 - -3.9 714,672 - -3.4 714,672 - -0.0 714,672 - -1.8 714,752 + -2.8 714,752 + Pf1N1B4_618 0.18 -0.0 714,752 + -4.1 714,770 + Pf1N1B4_618 0.23 -0.0 714,770 + Pf1N1B4_618 0.23 -3.0 714,770 + Pf1N1B4_618 0.23 -0.0 714,770 + -3.7 714,770 + -2.7 714,770 + Pf1N1B4_618 0.23 -0.0 714,771 - Pf1N1B4_618 0.23 -2.8 714,771 - -0.0 714,771 - Pf1N1B4_618 0.23 -0.0 714,771 - Pf1N1B4_618 0.23 -2.1 714,771 - Pf1N1B4_618 0.23 -2.3 714,771 - Pf1N1B4_618 0.23 -0.0 714,807 + Pf1N1B4_618 0.33 -0.0 714,881 + Pf1N1B4_618 0.54 -0.0 714,881 + Pf1N1B4_618 0.54 -2.8 714,881 + Pf1N1B4_618 0.54 -0.0 714,881 + Pf1N1B4_618 0.54 -0.0 714,881 + Pf1N1B4_618 0.54 -0.0 714,920 + Pf1N1B4_618 0.64 -0.0 714,975 + Pf1N1B4_618 0.80 -0.0 715,073 + -3.3 715,227 + -1.2 715,227 + -2.3 715,228 - -1.6 715,228 - -2.6 715,379 + -2.9 715,380 - +0.4
Or see this region's nucleotide sequence