Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_6039

Experiment: Nitrate reduction, replicate C t3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_6037 and Pf1N1B4_6038 are separated by 213 nucleotidesPf1N1B4_6038 and Pf1N1B4_6039 are separated by 4 nucleotidesPf1N1B4_6039 and Pf1N1B4_6040 are separated by 74 nucleotidesPf1N1B4_6040 and Pf1N1B4_6041 are separated by 249 nucleotides Pf1N1B4_6037 - L-asparaginase (EC 3.5.1.1), at 501,468 to 502,556 _6037 Pf1N1B4_6038 - FIG00953874: hypothetical protein, at 502,770 to 503,009 _6038 Pf1N1B4_6039 - Endonuclease I precursor (EC 3.1.21.1), at 503,014 to 503,703 _6039 Pf1N1B4_6040 - FIG00956858: hypothetical protein, at 503,778 to 504,143 _6040 Pf1N1B4_6041 - Carbon storage regulator, at 504,393 to 504,587 _6041 Position (kb) 503 504Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 502.230 kb on + strand, within Pf1N1B4_6037at 502.255 kb on - strandat 502.366 kb on + strand, within Pf1N1B4_6037at 502.366 kb on + strand, within Pf1N1B4_6037at 502.408 kb on + strandat 502.440 kb on + strand, within Pf1N1B4_6037at 502.440 kb on + strand, within Pf1N1B4_6037at 502.441 kb on - strand, within Pf1N1B4_6037at 502.551 kb on + strandat 502.551 kb on + strandat 502.551 kb on + strandat 502.552 kb on - strandat 502.660 kb on - strandat 502.705 kb on - strandat 502.734 kb on + strandat 502.764 kb on - strandat 502.796 kb on + strandat 502.796 kb on + strand, within Pf1N1B4_6038at 502.800 kb on + strand, within Pf1N1B4_6038at 502.800 kb on + strand, within Pf1N1B4_6038at 502.800 kb on + strand, within Pf1N1B4_6038at 502.801 kb on - strand, within Pf1N1B4_6038at 502.801 kb on - strand, within Pf1N1B4_6038at 502.801 kb on - strand, within Pf1N1B4_6038at 502.801 kb on - strand, within Pf1N1B4_6038at 503.036 kb on + strandat 503.119 kb on + strand, within Pf1N1B4_6039at 503.144 kb on - strandat 503.158 kb on + strand, within Pf1N1B4_6039at 503.158 kb on + strand, within Pf1N1B4_6039at 503.159 kb on - strandat 503.195 kb on - strandat 503.195 kb on - strand, within Pf1N1B4_6039at 503.329 kb on + strand, within Pf1N1B4_6039at 503.330 kb on - strand, within Pf1N1B4_6039at 503.330 kb on - strandat 503.437 kb on + strand, within Pf1N1B4_6039at 503.521 kb on + strand, within Pf1N1B4_6039at 503.522 kb on - strand, within Pf1N1B4_6039at 503.522 kb on - strand, within Pf1N1B4_6039at 503.522 kb on - strand, within Pf1N1B4_6039at 503.522 kb on - strand, within Pf1N1B4_6039at 503.527 kb on + strand, within Pf1N1B4_6039at 503.528 kb on - strand, within Pf1N1B4_6039at 503.528 kb on - strand, within Pf1N1B4_6039at 503.528 kb on - strand, within Pf1N1B4_6039at 503.535 kb on + strand, within Pf1N1B4_6039at 503.535 kb on + strand, within Pf1N1B4_6039at 503.578 kb on + strand, within Pf1N1B4_6039at 503.578 kb on + strand, within Pf1N1B4_6039at 503.579 kb on - strandat 503.579 kb on - strand, within Pf1N1B4_6039at 503.592 kb on + strand, within Pf1N1B4_6039at 503.599 kb on + strand, within Pf1N1B4_6039at 503.600 kb on - strand, within Pf1N1B4_6039at 503.889 kb on - strand, within Pf1N1B4_6040at 503.889 kb on - strand, within Pf1N1B4_6040at 503.948 kb on + strandat 503.949 kb on - strand, within Pf1N1B4_6040at 503.949 kb on - strandat 503.949 kb on - strand, within Pf1N1B4_6040at 503.949 kb on - strand, within Pf1N1B4_6040at 503.949 kb on - strand, within Pf1N1B4_6040at 503.949 kb on - strand, within Pf1N1B4_6040at 503.949 kb on - strand, within Pf1N1B4_6040at 503.949 kb on - strand, within Pf1N1B4_6040at 503.949 kb on - strand, within Pf1N1B4_6040at 503.949 kb on - strand, within Pf1N1B4_6040at 504.115 kb on - strandat 504.115 kb on - strandat 504.251 kb on - strandat 504.251 kb on - strandat 504.252 kb on + strandat 504.252 kb on + strandat 504.252 kb on + strandat 504.252 kb on + strandat 504.253 kb on - strandat 504.253 kb on - strandat 504.253 kb on - strandat 504.253 kb on - strandat 504.253 kb on - strandat 504.253 kb on - strandat 504.253 kb on - strandat 504.253 kb on - strandat 504.257 kb on - strandat 504.271 kb on - strandat 504.271 kb on - strandat 504.324 kb on - strandat 504.324 kb on - strandat 504.328 kb on + strandat 504.328 kb on + strandat 504.328 kb on + strandat 504.328 kb on + strandat 504.328 kb on + strandat 504.328 kb on + strandat 504.328 kb on + strandat 504.328 kb on + strandat 504.329 kb on - strandat 504.329 kb on - strandat 504.329 kb on - strandat 504.329 kb on - strandat 504.329 kb on - strandat 504.329 kb on - strandat 504.331 kb on - strandat 504.331 kb on - strandat 504.334 kb on + strandat 504.334 kb on + strandat 504.334 kb on + strandat 504.335 kb on - strandat 504.335 kb on - strandat 504.335 kb on - strandat 504.335 kb on - strandat 504.335 kb on - strandat 504.335 kb on - strandat 504.335 kb on - strandat 504.335 kb on - strandat 504.369 kb on + strandat 504.370 kb on - strandat 504.400 kb on + strandat 504.427 kb on + strand, within Pf1N1B4_6041at 504.428 kb on - strand, within Pf1N1B4_6041at 504.446 kb on + strand, within Pf1N1B4_6041at 504.447 kb on - strand, within Pf1N1B4_6041

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3
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502,230 + Pf1N1B4_6037 0.70 +0.0
502,255 - -1.1
502,366 + Pf1N1B4_6037 0.82 -1.9
502,366 + Pf1N1B4_6037 0.82 +0.0
502,408 + -3.0
502,440 + Pf1N1B4_6037 0.89 +0.0
502,440 + Pf1N1B4_6037 0.89 -2.4
502,441 - Pf1N1B4_6037 0.89 +0.0
502,551 + -1.6
502,551 + +0.0
502,551 + -1.0
502,552 - +0.0
502,660 - +0.0
502,705 - -5.7
502,734 + -3.0
502,764 - -2.7
502,796 + -1.6
502,796 + Pf1N1B4_6038 0.11 +0.0
502,800 + Pf1N1B4_6038 0.12 -1.5
502,800 + Pf1N1B4_6038 0.12 +0.0
502,800 + Pf1N1B4_6038 0.12 +0.0
502,801 - Pf1N1B4_6038 0.13 -2.0
502,801 - Pf1N1B4_6038 0.13 -3.1
502,801 - Pf1N1B4_6038 0.13 -2.6
502,801 - Pf1N1B4_6038 0.13 +0.0
503,036 + +0.0
503,119 + Pf1N1B4_6039 0.15 +0.0
503,144 - -2.3
503,158 + Pf1N1B4_6039 0.21 -2.1
503,158 + Pf1N1B4_6039 0.21 -1.5
503,159 - -2.7
503,195 - +0.0
503,195 - Pf1N1B4_6039 0.26 +0.0
503,329 + Pf1N1B4_6039 0.46 -1.8
503,330 - Pf1N1B4_6039 0.46 +0.0
503,330 - +0.0
503,437 + Pf1N1B4_6039 0.61 -3.2
503,521 + Pf1N1B4_6039 0.73 +0.0
503,522 - Pf1N1B4_6039 0.74 +0.0
503,522 - Pf1N1B4_6039 0.74 +0.0
503,522 - Pf1N1B4_6039 0.74 +0.0
503,522 - Pf1N1B4_6039 0.74 +0.0
503,527 + Pf1N1B4_6039 0.74 +0.0
503,528 - Pf1N1B4_6039 0.74 +0.0
503,528 - Pf1N1B4_6039 0.74 +0.0
503,528 - Pf1N1B4_6039 0.74 +0.0
503,535 + Pf1N1B4_6039 0.76 -1.5
503,535 + Pf1N1B4_6039 0.76 +0.0
503,578 + Pf1N1B4_6039 0.82 +0.0
503,578 + Pf1N1B4_6039 0.82 +0.0
503,579 - -2.6
503,579 - Pf1N1B4_6039 0.82 -0.4
503,592 + Pf1N1B4_6039 0.84 -3.0
503,599 + Pf1N1B4_6039 0.85 +0.0
503,600 - Pf1N1B4_6039 0.85 +0.0
503,889 - Pf1N1B4_6040 0.30 +0.0
503,889 - Pf1N1B4_6040 0.30 +0.0
503,948 + +0.0
503,949 - Pf1N1B4_6040 0.47 +0.0
503,949 - -1.6
503,949 - Pf1N1B4_6040 0.47 +0.0
503,949 - Pf1N1B4_6040 0.47 -3.6
503,949 - Pf1N1B4_6040 0.47 +0.0
503,949 - Pf1N1B4_6040 0.47 -3.1
503,949 - Pf1N1B4_6040 0.47 +0.0
503,949 - Pf1N1B4_6040 0.47 +0.0
503,949 - Pf1N1B4_6040 0.47 +0.0
503,949 - Pf1N1B4_6040 0.47 -1.7
504,115 - +0.0
504,115 - +0.0
504,251 - +0.0
504,251 - -3.8
504,252 + +0.0
504,252 + -2.9
504,252 + +0.0
504,252 + +0.0
504,253 - +0.0
504,253 - +0.0
504,253 - +0.0
504,253 - -2.7
504,253 - +0.0
504,253 - -4.9
504,253 - +0.0
504,253 - +0.0
504,257 - -2.7
504,271 - +0.0
504,271 - +0.0
504,324 - +0.0
504,324 - +0.0
504,328 + -3.3
504,328 + +0.0
504,328 + +0.0
504,328 + -2.3
504,328 + -2.3
504,328 + +0.0
504,328 + -3.3
504,328 + -3.6
504,329 - +0.0
504,329 - -2.0
504,329 - +0.0
504,329 - -2.7
504,329 - +0.0
504,329 - +0.0
504,331 - -3.3
504,331 - +0.0
504,334 + -1.7
504,334 + +0.0
504,334 + +0.0
504,335 - -5.0
504,335 - +0.0
504,335 - -3.5
504,335 - +0.0
504,335 - +0.0
504,335 - +0.0
504,335 - +0.0
504,335 - -4.0
504,369 + +0.0
504,370 - +1.0
504,400 + +0.0
504,427 + Pf1N1B4_6041 0.17 +0.0
504,428 - Pf1N1B4_6041 0.18 +0.0
504,446 + Pf1N1B4_6041 0.27 +0.0
504,447 - Pf1N1B4_6041 0.28 +0.0

Or see this region's nucleotide sequence