Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_602 and Pf1N1B4_603 are separated by 55 nucleotides Pf1N1B4_603 and Pf1N1B4_604 are separated by 77 nucleotides Pf1N1B4_604 and Pf1N1B4_605 are separated by 28 nucleotides Pf1N1B4_605 and Pf1N1B4_606 overlap by 69 nucleotides
Pf1N1B4_602 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 695,951 to 696,952
_602
Pf1N1B4_603 - Methylglyoxal synthase (EC 4.2.3.3), at 697,008 to 697,472
_603
Pf1N1B4_604 - FIG006045: Sigma factor, ECF subfamily, at 697,550 to 698,068
_604
Pf1N1B4_605 - hypothetical protein, at 698,097 to 698,288
_605
Pf1N1B4_606 - Iron siderophore sensor protein, at 698,220 to 699,128
_606
Position (kb) 697
698 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 696.015 kb on - strand at 696.098 kb on + strand, within Pf1N1B4_602 at 696.172 kb on + strand at 696.172 kb on + strand, within Pf1N1B4_602 at 696.286 kb on + strand, within Pf1N1B4_602 at 696.287 kb on - strand at 696.313 kb on + strand, within Pf1N1B4_602 at 696.314 kb on - strand, within Pf1N1B4_602 at 696.497 kb on + strand, within Pf1N1B4_602 at 696.498 kb on - strand, within Pf1N1B4_602 at 696.950 kb on + strand at 697.088 kb on - strand at 697.389 kb on + strand, within Pf1N1B4_603 at 697.389 kb on + strand, within Pf1N1B4_603 at 697.389 kb on + strand, within Pf1N1B4_603 at 697.390 kb on - strand, within Pf1N1B4_603 at 697.390 kb on - strand, within Pf1N1B4_603 at 697.400 kb on + strand, within Pf1N1B4_603 at 697.400 kb on + strand, within Pf1N1B4_603 at 697.401 kb on - strand, within Pf1N1B4_603 at 697.401 kb on - strand, within Pf1N1B4_603 at 697.444 kb on - strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.453 kb on - strand at 697.453 kb on - strand at 697.453 kb on - strand at 697.471 kb on - strand at 697.664 kb on + strand, within Pf1N1B4_604 at 697.665 kb on - strand, within Pf1N1B4_604 at 697.665 kb on - strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 698.098 kb on + strand at 698.098 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 696,015 - -1.6 696,098 + Pf1N1B4_602 0.15 -0.0 696,172 + -2.5 696,172 + Pf1N1B4_602 0.22 -1.8 696,286 + Pf1N1B4_602 0.33 -0.0 696,287 - -0.0 696,313 + Pf1N1B4_602 0.36 -2.3 696,314 - Pf1N1B4_602 0.36 -0.0 696,497 + Pf1N1B4_602 0.54 -2.0 696,498 - Pf1N1B4_602 0.55 -0.0 696,950 + -0.0 697,088 - -2.6 697,389 + Pf1N1B4_603 0.82 -4.5 697,389 + Pf1N1B4_603 0.82 -0.0 697,389 + Pf1N1B4_603 0.82 -3.0 697,390 - Pf1N1B4_603 0.82 -0.0 697,390 - Pf1N1B4_603 0.82 -0.0 697,400 + Pf1N1B4_603 0.84 -0.0 697,400 + Pf1N1B4_603 0.84 -3.0 697,401 - Pf1N1B4_603 0.85 -2.9 697,401 - Pf1N1B4_603 0.85 -3.1 697,444 - -0.0 697,452 + -0.0 697,452 + -2.3 697,452 + -3.7 697,452 + -1.4 697,453 - -0.0 697,453 - -2.3 697,453 - -0.0 697,471 - -0.0 697,664 + Pf1N1B4_604 0.22 -0.0 697,665 - Pf1N1B4_604 0.22 -0.0 697,665 - Pf1N1B4_604 0.22 -1.7 697,958 + Pf1N1B4_604 0.79 -4.7 697,958 + Pf1N1B4_604 0.79 -1.3 697,958 + Pf1N1B4_604 0.79 -2.8 697,958 + Pf1N1B4_604 0.79 -0.0 697,959 - Pf1N1B4_604 0.79 -0.0 697,959 - Pf1N1B4_604 0.79 -0.0 697,959 - Pf1N1B4_604 0.79 -0.0 697,959 - Pf1N1B4_604 0.79 -0.0 698,098 + -0.0 698,098 + -0.0
Or see this region's nucleotide sequence