Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5877 and Pf1N1B4_5878 are separated by 86 nucleotides Pf1N1B4_5878 and Pf1N1B4_5879 are separated by 10 nucleotides Pf1N1B4_5879 and Pf1N1B4_5880 are separated by 163 nucleotides Pf1N1B4_5880 and Pf1N1B4_5881 overlap by 11 nucleotides
Pf1N1B4_5877 - FIG00967533: hypothetical protein, at 318,840 to 319,424
_5877
Pf1N1B4_5878 - hypothetical protein, at 319,511 to 319,684
_5878
Pf1N1B4_5879 - hypothetical protein, at 319,695 to 319,937
_5879
Pf1N1B4_5880 - AttH component of AttEFGH ABC transport system, at 320,101 to 321,174
_5880
Pf1N1B4_5881 - AttF component of AttEFGH ABC transport system / AttG component of AttEFGH ABC transport system, at 321,164 to 323,635
_5881
Position (kb) 320
321
322 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 319.144 kb on + strand, within Pf1N1B4_5877 at 319.230 kb on + strand, within Pf1N1B4_5877 at 319.230 kb on + strand, within Pf1N1B4_5877 at 319.230 kb on + strand, within Pf1N1B4_5877 at 319.231 kb on - strand, within Pf1N1B4_5877 at 319.424 kb on + strand at 319.425 kb on - strand at 319.431 kb on + strand at 319.467 kb on + strand at 319.501 kb on + strand at 319.502 kb on - strand at 319.502 kb on - strand at 319.502 kb on - strand at 319.833 kb on + strand, within Pf1N1B4_5879 at 319.957 kb on + strand at 319.958 kb on - strand at 320.010 kb on - strand at 320.059 kb on - strand at 320.079 kb on + strand at 320.079 kb on + strand at 320.080 kb on - strand at 320.102 kb on + strand at 320.103 kb on - strand at 320.103 kb on - strand at 320.124 kb on - strand at 320.372 kb on + strand, within Pf1N1B4_5880 at 320.522 kb on + strand, within Pf1N1B4_5880 at 320.523 kb on - strand at 320.576 kb on + strand, within Pf1N1B4_5880 at 320.984 kb on + strand, within Pf1N1B4_5880 at 320.984 kb on + strand at 320.984 kb on + strand, within Pf1N1B4_5880 at 321.063 kb on + strand at 321.165 kb on + strand at 321.296 kb on + strand at 321.453 kb on - strand, within Pf1N1B4_5881 at 321.975 kb on + strand, within Pf1N1B4_5881 at 321.975 kb on + strand, within Pf1N1B4_5881 at 321.976 kb on - strand, within Pf1N1B4_5881 at 322.128 kb on + strand, within Pf1N1B4_5881
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 319,144 + Pf1N1B4_5877 0.52 -3.8 319,230 + Pf1N1B4_5877 0.67 +0.0 319,230 + Pf1N1B4_5877 0.67 -2.9 319,230 + Pf1N1B4_5877 0.67 +0.0 319,231 - Pf1N1B4_5877 0.67 +0.0 319,424 + +0.0 319,425 - +0.0 319,431 + -5.6 319,467 + -3.8 319,501 + -2.4 319,502 - +0.0 319,502 - -3.1 319,502 - -1.8 319,833 + Pf1N1B4_5879 0.57 +0.0 319,957 + +0.0 319,958 - +0.0 320,010 - -2.2 320,059 - +0.0 320,079 + -2.3 320,079 + +0.0 320,080 - +0.0 320,102 + +0.0 320,103 - -1.8 320,103 - -2.7 320,124 - -1.6 320,372 + Pf1N1B4_5880 0.25 -2.7 320,522 + Pf1N1B4_5880 0.39 -3.5 320,523 - -1.0 320,576 + Pf1N1B4_5880 0.44 +0.0 320,984 + Pf1N1B4_5880 0.82 -3.1 320,984 + -2.2 320,984 + Pf1N1B4_5880 0.82 +0.0 321,063 + +0.0 321,165 + -2.6 321,296 + -2.3 321,453 - Pf1N1B4_5881 0.12 +0.0 321,975 + Pf1N1B4_5881 0.33 -3.6 321,975 + Pf1N1B4_5881 0.33 +0.0 321,976 - Pf1N1B4_5881 0.33 +0.0 322,128 + Pf1N1B4_5881 0.39 -5.1
Or see this region's nucleotide sequence