Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5759 and Pf1N1B4_5760 are separated by 3 nucleotides Pf1N1B4_5760 and Pf1N1B4_5761 are separated by 79 nucleotides Pf1N1B4_5761 and Pf1N1B4_5762 are separated by 11 nucleotides Pf1N1B4_5762 and Pf1N1B4_5763 are separated by 43 nucleotides
Pf1N1B4_5759 - Nitric-oxide reductase subunit B (EC 1.7.99.7), at 199,183 to 200,610
_5759
Pf1N1B4_5760 - Nitric-oxide reductase subunit C (EC 1.7.99.7), at 200,614 to 201,222
_5760
Pf1N1B4_5761 - Nitric oxide reductase activation protein NorF, at 201,302 to 201,559
_5761
Pf1N1B4_5762 - Nitric oxide reductase activation protein NorE, at 201,571 to 202,098
_5762
Pf1N1B4_5763 - Nitric oxide reductase activation protein NorQ, at 202,142 to 202,933
_5763
Position (kb) 200
201
202 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 199.669 kb on - strand, within Pf1N1B4_5759 at 199.887 kb on - strand, within Pf1N1B4_5759 at 199.887 kb on - strand, within Pf1N1B4_5759 at 199.887 kb on - strand, within Pf1N1B4_5759 at 200.010 kb on - strand, within Pf1N1B4_5759 at 200.043 kb on - strand, within Pf1N1B4_5759 at 200.144 kb on + strand, within Pf1N1B4_5759 at 200.382 kb on - strand at 200.489 kb on + strand at 200.615 kb on + strand at 200.616 kb on - strand at 200.640 kb on + strand at 200.641 kb on - strand at 200.641 kb on - strand at 200.770 kb on + strand, within Pf1N1B4_5760 at 200.770 kb on + strand, within Pf1N1B4_5760 at 200.770 kb on + strand, within Pf1N1B4_5760 at 201.015 kb on - strand at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.015 kb on - strand, within Pf1N1B4_5760 at 201.018 kb on + strand, within Pf1N1B4_5760 at 201.087 kb on + strand, within Pf1N1B4_5760 at 201.330 kb on - strand, within Pf1N1B4_5761 at 201.633 kb on - strand, within Pf1N1B4_5762 at 201.730 kb on + strand, within Pf1N1B4_5762 at 201.730 kb on + strand, within Pf1N1B4_5762 at 201.731 kb on - strand, within Pf1N1B4_5762 at 201.846 kb on - strand, within Pf1N1B4_5762 at 201.878 kb on + strand, within Pf1N1B4_5762 at 201.878 kb on + strand, within Pf1N1B4_5762 at 201.879 kb on - strand, within Pf1N1B4_5762
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 199,669 - Pf1N1B4_5759 0.34 -3.4 199,887 - Pf1N1B4_5759 0.49 +0.1 199,887 - Pf1N1B4_5759 0.49 -5.3 199,887 - Pf1N1B4_5759 0.49 -6.5 200,010 - Pf1N1B4_5759 0.58 +0.1 200,043 - Pf1N1B4_5759 0.60 +0.1 200,144 + Pf1N1B4_5759 0.67 +0.1 200,382 - +0.1 200,489 + -5.4 200,615 + -5.5 200,616 - +0.1 200,640 + -5.1 200,641 - +0.1 200,641 - -4.4 200,770 + Pf1N1B4_5760 0.26 +0.1 200,770 + Pf1N1B4_5760 0.26 +0.1 200,770 + Pf1N1B4_5760 0.26 +0.1 201,015 - +0.1 201,015 - Pf1N1B4_5760 0.66 -5.5 201,015 - Pf1N1B4_5760 0.66 +0.1 201,015 - Pf1N1B4_5760 0.66 -3.0 201,018 + Pf1N1B4_5760 0.66 -2.8 201,087 + Pf1N1B4_5760 0.78 +0.1 201,330 - Pf1N1B4_5761 0.11 -3.8 201,633 - Pf1N1B4_5762 0.12 -2.9 201,730 + Pf1N1B4_5762 0.30 +0.1 201,730 + Pf1N1B4_5762 0.30 +0.1 201,731 - Pf1N1B4_5762 0.30 -4.8 201,846 - Pf1N1B4_5762 0.52 -5.7 201,878 + Pf1N1B4_5762 0.58 +0.1 201,878 + Pf1N1B4_5762 0.58 -4.8 201,879 - Pf1N1B4_5762 0.58 +0.1
Or see this region's nucleotide sequence