Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5722 and Pf1N1B4_5723 are separated by 0 nucleotides Pf1N1B4_5723 and Pf1N1B4_5724 are separated by 34 nucleotides Pf1N1B4_5724 and Pf1N1B4_5725 overlap by 16 nucleotides Pf1N1B4_5725 and Pf1N1B4_5726 are separated by 38 nucleotides
Pf1N1B4_5722 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,711 to 164,091
_5722
Pf1N1B4_5723 - putative exported protein, at 164,092 to 164,424
_5723
Pf1N1B4_5724 - High-affinity iron permease, at 164,459 to 164,941
_5724
Pf1N1B4_5725 - High-affinity iron permease, at 164,926 to 165,300
_5725
Pf1N1B4_5726 - Ferredoxin, at 165,339 to 166,667
_5726
Position (kb) 164
165
166 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 163.936 kb on - strand, within Pf1N1B4_5722 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.801 kb on + strand, within Pf1N1B4_5724 at 164.802 kb on - strand, within Pf1N1B4_5724 at 165.101 kb on - strand, within Pf1N1B4_5725 at 165.385 kb on + strand at 165.394 kb on - strand at 165.485 kb on - strand, within Pf1N1B4_5726 at 165.594 kb on + strand, within Pf1N1B4_5726 at 165.699 kb on + strand, within Pf1N1B4_5726 at 166.054 kb on + strand, within Pf1N1B4_5726
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 163,936 - Pf1N1B4_5722 0.59 +0.1 164,798 + Pf1N1B4_5724 0.70 +0.1 164,798 + Pf1N1B4_5724 0.70 -2.6 164,798 + Pf1N1B4_5724 0.70 +0.1 164,801 + Pf1N1B4_5724 0.71 -1.2 164,802 - Pf1N1B4_5724 0.71 -2.6 165,101 - Pf1N1B4_5725 0.47 -0.5 165,385 + -2.7 165,394 - -3.3 165,485 - Pf1N1B4_5726 0.11 -1.5 165,594 + Pf1N1B4_5726 0.19 -2.3 165,699 + Pf1N1B4_5726 0.27 +0.1 166,054 + Pf1N1B4_5726 0.54 -3.0
Or see this region's nucleotide sequence