Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5405 and Pf1N1B4_5406 are separated by 875 nucleotides Pf1N1B4_5406 and Pf1N1B4_5407 are separated by 379 nucleotides Pf1N1B4_5407 and Pf1N1B4_5408 are separated by 69 nucleotides Pf1N1B4_5408 and Pf1N1B4_5409 are separated by 38 nucleotides
Pf1N1B4_5405 - hypothetical protein, at 140,141 to 141,181
_5405
Pf1N1B4_5406 - hypothetical protein, at 142,057 to 142,173
_5406
Pf1N1B4_5407 - hypothetical protein, at 142,553 to 142,762
_5407
Pf1N1B4_5408 - hypothetical protein, at 142,832 to 143,065
_5408
Pf1N1B4_5409 - hypothetical protein, at 143,104 to 143,562
_5409
Position (kb) 142
143 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 141.074 kb on + strand, within Pf1N1B4_5405 at 141.074 kb on + strand, within Pf1N1B4_5405 at 141.074 kb on + strand, within Pf1N1B4_5405 at 141.074 kb on + strand, within Pf1N1B4_5405 at 141.074 kb on + strand, within Pf1N1B4_5405 at 141.075 kb on - strand, within Pf1N1B4_5405 at 141.088 kb on + strand at 141.088 kb on + strand at 141.089 kb on - strand at 141.089 kb on - strand at 141.092 kb on + strand at 141.092 kb on + strand at 141.092 kb on + strand at 141.092 kb on + strand at 141.092 kb on + strand at 141.092 kb on + strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.093 kb on - strand at 141.131 kb on + strand at 141.134 kb on + strand at 141.156 kb on + strand at 141.156 kb on + strand at 141.156 kb on + strand at 141.157 kb on - strand at 141.157 kb on - strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.161 kb on - strand at 141.296 kb on + strand at 141.297 kb on - strand at 141.297 kb on - strand at 141.350 kb on - strand at 141.350 kb on - strand at 141.350 kb on - strand at 141.363 kb on - strand at 141.415 kb on + strand at 141.415 kb on + strand at 141.415 kb on + strand at 141.415 kb on + strand at 141.415 kb on + strand at 141.416 kb on - strand at 141.416 kb on - strand at 141.416 kb on - strand at 141.416 kb on - strand at 141.427 kb on + strand at 141.428 kb on - strand at 141.436 kb on + strand at 141.437 kb on - strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.438 kb on + strand at 141.439 kb on - strand at 141.439 kb on - strand at 141.439 kb on - strand at 141.439 kb on - strand at 141.439 kb on - strand at 141.439 kb on - strand at 141.487 kb on - strand at 141.621 kb on + strand at 141.621 kb on + strand at 141.621 kb on + strand at 141.621 kb on + strand at 141.621 kb on + strand at 141.622 kb on - strand at 141.624 kb on + strand at 141.625 kb on - strand at 141.625 kb on - strand at 141.653 kb on + strand at 141.654 kb on - strand at 141.654 kb on - strand at 141.654 kb on - strand at 141.654 kb on - strand at 141.654 kb on - strand at 141.668 kb on + strand at 141.668 kb on + strand at 141.668 kb on + strand at 141.668 kb on + strand at 141.669 kb on - strand at 141.669 kb on - strand at 141.669 kb on - strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.725 kb on + strand at 141.726 kb on - strand at 141.726 kb on - strand at 141.726 kb on - strand at 141.726 kb on - strand at 141.726 kb on - strand at 141.726 kb on - strand at 141.774 kb on + strand at 141.791 kb on + strand at 141.792 kb on - strand at 141.813 kb on + strand at 141.816 kb on - strand at 141.816 kb on - strand at 141.899 kb on + strand at 141.899 kb on + strand at 141.900 kb on - strand at 141.900 kb on - strand at 141.920 kb on - strand at 141.970 kb on + strand at 141.971 kb on - strand at 141.971 kb on - strand at 141.995 kb on + strand at 141.996 kb on - strand at 141.996 kb on - strand at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.098 kb on + strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.099 kb on - strand, within Pf1N1B4_5406 at 142.268 kb on + strand at 142.269 kb on - strand at 142.269 kb on - strand at 142.357 kb on + strand at 142.357 kb on + strand at 142.358 kb on - strand at 142.452 kb on - strand at 142.523 kb on - strand at 142.593 kb on + strand, within Pf1N1B4_5407 at 142.665 kb on + strand, within Pf1N1B4_5407 at 142.665 kb on + strand, within Pf1N1B4_5407 at 142.666 kb on - strand, within Pf1N1B4_5407 at 142.666 kb on - strand, within Pf1N1B4_5407 at 142.666 kb on - strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.766 kb on + strand at 142.767 kb on - strand at 142.767 kb on - strand at 142.819 kb on - strand at 142.877 kb on + strand, within Pf1N1B4_5408 at 142.877 kb on + strand, within Pf1N1B4_5408 at 142.877 kb on + strand, within Pf1N1B4_5408 at 142.878 kb on - strand, within Pf1N1B4_5408 at 142.878 kb on - strand, within Pf1N1B4_5408 at 142.878 kb on - strand at 142.879 kb on + strand, within Pf1N1B4_5408 at 142.879 kb on + strand at 142.880 kb on - strand, within Pf1N1B4_5408 at 142.982 kb on - strand, within Pf1N1B4_5408 at 142.989 kb on + strand, within Pf1N1B4_5408 at 142.989 kb on + strand, within Pf1N1B4_5408 at 142.990 kb on - strand, within Pf1N1B4_5408 at 143.064 kb on - strand at 143.081 kb on + strand at 143.134 kb on - strand at 143.134 kb on - strand at 143.134 kb on - strand at 143.169 kb on - strand, within Pf1N1B4_5409
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 141,074 + Pf1N1B4_5405 0.90 -2.2 141,074 + Pf1N1B4_5405 0.90 +0.0 141,074 + Pf1N1B4_5405 0.90 -1.1 141,074 + Pf1N1B4_5405 0.90 +0.0 141,074 + Pf1N1B4_5405 0.90 +0.0 141,075 - Pf1N1B4_5405 0.90 +0.0 141,088 + -2.8 141,088 + +0.0 141,089 - +0.0 141,089 - +0.0 141,092 + -2.9 141,092 + +0.0 141,092 + +0.0 141,092 + +0.0 141,092 + -1.8 141,092 + -2.5 141,093 - +0.0 141,093 - -2.7 141,093 - +0.0 141,093 - +0.0 141,093 - -2.6 141,093 - -1.8 141,093 - +0.0 141,131 + +0.0 141,134 + +0.0 141,156 + +0.0 141,156 + -2.9 141,156 + +0.0 141,157 - -1.6 141,157 - +0.0 141,160 + +0.0 141,160 + -3.9 141,160 + +0.0 141,160 + -2.5 141,160 + +0.0 141,160 + +0.0 141,160 + +0.0 141,161 - +0.0 141,296 + -2.9 141,297 - +0.0 141,297 - -2.9 141,350 - +0.0 141,350 - -1.7 141,350 - +0.0 141,363 - -2.1 141,415 + +0.0 141,415 + -3.4 141,415 + +0.0 141,415 + +0.0 141,415 + -5.5 141,416 - -3.0 141,416 - +0.0 141,416 - +0.0 141,416 - +0.0 141,427 + +0.0 141,428 - +0.0 141,436 + +0.0 141,437 - -2.9 141,438 + +0.0 141,438 + -2.4 141,438 + +0.0 141,438 + -2.3 141,438 + -2.0 141,438 + +0.0 141,438 + -2.0 141,438 + +0.0 141,438 + -1.2 141,438 + -2.9 141,438 + +0.0 141,439 - -3.4 141,439 - +0.0 141,439 - -1.7 141,439 - +0.0 141,439 - +0.0 141,439 - +0.0 141,487 - +0.0 141,621 + +0.0 141,621 + -3.3 141,621 + -2.2 141,621 + -3.7 141,621 + +0.0 141,622 - -4.6 141,624 + -2.8 141,625 - +0.0 141,625 - +0.0 141,653 + +0.0 141,654 - +0.0 141,654 - -5.4 141,654 - +0.0 141,654 - +0.0 141,654 - +0.0 141,668 + -2.4 141,668 + +0.0 141,668 + -2.7 141,668 + -2.9 141,669 - +0.0 141,669 - +0.0 141,669 - -2.6 141,725 + +0.0 141,725 + -2.6 141,725 + -3.1 141,725 + +0.0 141,725 + +0.0 141,725 + +0.0 141,725 + -4.3 141,725 + -2.6 141,725 + -5.9 141,726 - +0.0 141,726 - -2.2 141,726 - -1.5 141,726 - -2.4 141,726 - +0.0 141,726 - -2.5 141,774 + +0.0 141,791 + -3.4 141,792 - -2.5 141,813 + +0.0 141,816 - +0.0 141,816 - -2.7 141,899 + +0.0 141,899 + +0.0 141,900 - +0.0 141,900 - -4.3 141,920 - +0.0 141,970 + -2.5 141,971 - -3.1 141,971 - -3.4 141,995 + -3.8 141,996 - -3.5 141,996 - -2.9 142,098 + Pf1N1B4_5406 0.35 +0.0 142,098 + Pf1N1B4_5406 0.35 +0.0 142,098 + Pf1N1B4_5406 0.35 -2.5 142,098 + Pf1N1B4_5406 0.35 -4.0 142,098 + Pf1N1B4_5406 0.35 +0.0 142,098 + Pf1N1B4_5406 0.35 +0.0 142,098 + Pf1N1B4_5406 0.35 -3.5 142,099 - Pf1N1B4_5406 0.36 -1.6 142,099 - Pf1N1B4_5406 0.36 +0.0 142,099 - Pf1N1B4_5406 0.36 +0.0 142,099 - Pf1N1B4_5406 0.36 +0.0 142,099 - Pf1N1B4_5406 0.36 -1.5 142,099 - Pf1N1B4_5406 0.36 +0.0 142,099 - Pf1N1B4_5406 0.36 +0.0 142,268 + +0.0 142,269 - +0.0 142,269 - -5.4 142,357 + +0.0 142,357 + -2.4 142,358 - +0.0 142,452 - +0.0 142,523 - +0.0 142,593 + Pf1N1B4_5407 0.19 +0.0 142,665 + Pf1N1B4_5407 0.53 -2.5 142,665 + Pf1N1B4_5407 0.53 -2.4 142,666 - Pf1N1B4_5407 0.54 +0.0 142,666 - Pf1N1B4_5407 0.54 +0.0 142,666 - +0.0 142,766 + -3.7 142,766 + -3.1 142,766 + +0.0 142,766 + -2.4 142,766 + +0.0 142,766 + -2.3 142,767 - +0.0 142,767 - +0.0 142,819 - -1.5 142,877 + Pf1N1B4_5408 0.19 +0.0 142,877 + Pf1N1B4_5408 0.19 +0.0 142,877 + Pf1N1B4_5408 0.19 +0.0 142,878 - Pf1N1B4_5408 0.20 -2.4 142,878 - Pf1N1B4_5408 0.20 +0.0 142,878 - +0.0 142,879 + Pf1N1B4_5408 0.20 -2.0 142,879 + -1.9 142,880 - Pf1N1B4_5408 0.21 -3.6 142,982 - Pf1N1B4_5408 0.64 +0.0 142,989 + Pf1N1B4_5408 0.67 +0.0 142,989 + Pf1N1B4_5408 0.67 -2.5 142,990 - Pf1N1B4_5408 0.68 +0.0 143,064 - +0.0 143,081 + -1.3 143,134 - -1.9 143,134 - -0.3 143,134 - -3.6 143,169 - Pf1N1B4_5409 0.14 +0.0
Or see this region's nucleotide sequence