Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5311 and Pf1N1B4_5312 are separated by 242 nucleotides Pf1N1B4_5312 and Pf1N1B4_5313 are separated by 40 nucleotides
Pf1N1B4_5311 - hypothetical protein, at 61,965 to 62,252
_5311
Pf1N1B4_5312 - Putative activity regulator of membrane protease YbbK, at 62,495 to 62,932
_5312
Pf1N1B4_5313 - Putative stomatin/prohibitin-family membrane protease subunit YbbK, at 62,973 to 63,839
_5313
Position (kb) 62
63 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 61.496 kb on + strand at 61.534 kb on + strand at 61.605 kb on - strand at 61.675 kb on + strand at 61.675 kb on + strand at 61.676 kb on - strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.680 kb on - strand at 61.714 kb on + strand at 61.714 kb on + strand at 61.723 kb on + strand at 61.866 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.921 kb on - strand at 61.921 kb on - strand at 61.921 kb on - strand at 62.067 kb on + strand, within Pf1N1B4_5311 at 62.068 kb on - strand, within Pf1N1B4_5311 at 62.113 kb on + strand, within Pf1N1B4_5311 at 62.113 kb on + strand, within Pf1N1B4_5311 at 62.279 kb on + strand at 62.279 kb on + strand at 62.279 kb on + strand at 62.279 kb on + strand at 62.323 kb on + strand at 62.323 kb on + strand at 62.323 kb on + strand at 62.323 kb on + strand at 62.324 kb on - strand at 62.324 kb on - strand at 62.324 kb on - strand at 62.324 kb on - strand at 62.329 kb on + strand at 62.330 kb on + strand at 62.330 kb on + strand at 62.331 kb on - strand at 62.372 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.388 kb on - strand at 62.388 kb on - strand at 62.388 kb on - strand at 62.421 kb on + strand at 62.421 kb on + strand at 62.421 kb on + strand at 62.421 kb on + strand at 62.430 kb on + strand at 62.456 kb on + strand at 62.456 kb on + strand at 62.457 kb on - strand at 62.614 kb on + strand, within Pf1N1B4_5312 at 62.614 kb on + strand at 62.615 kb on - strand, within Pf1N1B4_5312 at 62.634 kb on + strand, within Pf1N1B4_5312 at 62.634 kb on + strand, within Pf1N1B4_5312 at 62.635 kb on - strand, within Pf1N1B4_5312 at 62.725 kb on + strand, within Pf1N1B4_5312 at 62.826 kb on + strand, within Pf1N1B4_5312 at 62.826 kb on + strand, within Pf1N1B4_5312 at 62.827 kb on - strand, within Pf1N1B4_5312 at 62.892 kb on + strand at 62.892 kb on + strand at 62.892 kb on + strand at 62.892 kb on + strand at 62.893 kb on - strand at 62.952 kb on + strand at 62.952 kb on + strand at 62.952 kb on + strand at 62.968 kb on + strand at 62.968 kb on + strand at 62.968 kb on + strand at 62.968 kb on + strand at 62.971 kb on + strand at 62.971 kb on + strand at 62.972 kb on - strand at 63.131 kb on - strand, within Pf1N1B4_5313 at 63.262 kb on + strand, within Pf1N1B4_5313 at 63.262 kb on + strand, within Pf1N1B4_5313 at 63.262 kb on + strand, within Pf1N1B4_5313 at 63.262 kb on + strand, within Pf1N1B4_5313 at 63.395 kb on + strand, within Pf1N1B4_5313 at 63.395 kb on + strand, within Pf1N1B4_5313 at 63.445 kb on + strand, within Pf1N1B4_5313 at 63.445 kb on + strand, within Pf1N1B4_5313 at 63.445 kb on + strand, within Pf1N1B4_5313 at 63.446 kb on - strand, within Pf1N1B4_5313 at 63.446 kb on - strand at 63.449 kb on + strand, within Pf1N1B4_5313 at 63.450 kb on - strand, within Pf1N1B4_5313 at 63.514 kb on + strand, within Pf1N1B4_5313 at 63.547 kb on + strand, within Pf1N1B4_5313 at 63.547 kb on + strand, within Pf1N1B4_5313 at 63.564 kb on - strand, within Pf1N1B4_5313 at 63.711 kb on + strand at 63.711 kb on + strand, within Pf1N1B4_5313 at 63.712 kb on - strand at 63.729 kb on + strand, within Pf1N1B4_5313 at 63.729 kb on + strand, within Pf1N1B4_5313 at 63.730 kb on - strand, within Pf1N1B4_5313 at 63.731 kb on + strand, within Pf1N1B4_5313 at 63.731 kb on + strand, within Pf1N1B4_5313 at 63.731 kb on + strand, within Pf1N1B4_5313 at 63.731 kb on + strand, within Pf1N1B4_5313 at 63.731 kb on + strand, within Pf1N1B4_5313 at 63.731 kb on + strand, within Pf1N1B4_5313 at 63.731 kb on + strand, within Pf1N1B4_5313 at 63.731 kb on + strand, within Pf1N1B4_5313 at 63.732 kb on - strand, within Pf1N1B4_5313 at 63.732 kb on - strand, within Pf1N1B4_5313 at 63.820 kb on + strand at 63.821 kb on - strand at 63.821 kb on - strand at 63.898 kb on + strand at 63.899 kb on - strand at 63.914 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 61,496 + -2.4 61,534 + +0.0 61,605 - +0.0 61,675 + +0.0 61,675 + +0.0 61,676 - +0.0 61,679 + -1.9 61,679 + +0.0 61,679 + -3.4 61,679 + -4.2 61,679 + -3.2 61,679 + +0.0 61,679 + +0.0 61,679 + -1.7 61,679 + +0.0 61,680 - -1.0 61,714 + +0.0 61,714 + -1.7 61,723 + +0.0 61,866 + -2.0 61,920 + +0.0 61,920 + +0.0 61,920 + +0.0 61,920 + -3.5 61,920 + +0.0 61,920 + +0.0 61,920 + -2.8 61,920 + +0.0 61,920 + -3.4 61,920 + +0.0 61,920 + -3.5 61,920 + +0.0 61,920 + -2.5 61,920 + -5.5 61,920 + +0.0 61,921 - +0.0 61,921 - -2.5 61,921 - +0.0 62,067 + Pf1N1B4_5311 0.35 -4.5 62,068 - Pf1N1B4_5311 0.36 +0.0 62,113 + Pf1N1B4_5311 0.51 +0.0 62,113 + Pf1N1B4_5311 0.51 +0.0 62,279 + -3.1 62,279 + +0.0 62,279 + +0.0 62,279 + +0.0 62,323 + +0.0 62,323 + -3.3 62,323 + +0.0 62,323 + +0.0 62,324 - +0.0 62,324 - -3.6 62,324 - +0.0 62,324 - -3.1 62,329 + +0.0 62,330 + +0.0 62,330 + +0.0 62,331 - +0.0 62,372 + +0.0 62,387 + -2.9 62,387 + -1.6 62,387 + +0.0 62,387 + -2.5 62,387 + -2.8 62,387 + +0.0 62,387 + +0.0 62,387 + +0.0 62,387 + +0.0 62,387 + -2.5 62,388 - +0.0 62,388 - +0.0 62,388 - -1.5 62,421 + -3.2 62,421 + +0.0 62,421 + -3.3 62,421 + -2.5 62,430 + +0.0 62,456 + +0.0 62,456 + +0.0 62,457 - +0.0 62,614 + Pf1N1B4_5312 0.27 +0.0 62,614 + +0.0 62,615 - Pf1N1B4_5312 0.27 -6.2 62,634 + Pf1N1B4_5312 0.32 -2.6 62,634 + Pf1N1B4_5312 0.32 +0.0 62,635 - Pf1N1B4_5312 0.32 -4.1 62,725 + Pf1N1B4_5312 0.53 +0.0 62,826 + Pf1N1B4_5312 0.76 -4.0 62,826 + Pf1N1B4_5312 0.76 +0.0 62,827 - Pf1N1B4_5312 0.76 -3.7 62,892 + -3.6 62,892 + -4.4 62,892 + +0.0 62,892 + -2.8 62,893 - -3.9 62,952 + +0.0 62,952 + -2.4 62,952 + +0.0 62,968 + +0.0 62,968 + +0.0 62,968 + +0.0 62,968 + +0.0 62,971 + +0.0 62,971 + +0.0 62,972 - +0.0 63,131 - Pf1N1B4_5313 0.18 -2.6 63,262 + Pf1N1B4_5313 0.33 -4.0 63,262 + Pf1N1B4_5313 0.33 +0.0 63,262 + Pf1N1B4_5313 0.33 -1.8 63,262 + Pf1N1B4_5313 0.33 -3.4 63,395 + Pf1N1B4_5313 0.49 +0.0 63,395 + Pf1N1B4_5313 0.49 +0.0 63,445 + Pf1N1B4_5313 0.54 +0.0 63,445 + Pf1N1B4_5313 0.54 -4.9 63,445 + Pf1N1B4_5313 0.54 -3.8 63,446 - Pf1N1B4_5313 0.55 +0.0 63,446 - +0.0 63,449 + Pf1N1B4_5313 0.55 +0.0 63,450 - Pf1N1B4_5313 0.55 -3.0 63,514 + Pf1N1B4_5313 0.62 +0.0 63,547 + Pf1N1B4_5313 0.66 +0.0 63,547 + Pf1N1B4_5313 0.66 +0.0 63,564 - Pf1N1B4_5313 0.68 -1.9 63,711 + +0.0 63,711 + Pf1N1B4_5313 0.85 +0.0 63,712 - -1.6 63,729 + Pf1N1B4_5313 0.87 -2.6 63,729 + Pf1N1B4_5313 0.87 +0.0 63,730 - Pf1N1B4_5313 0.87 +0.0 63,731 + Pf1N1B4_5313 0.87 +0.0 63,731 + Pf1N1B4_5313 0.87 +0.0 63,731 + Pf1N1B4_5313 0.87 +0.0 63,731 + Pf1N1B4_5313 0.87 -5.2 63,731 + Pf1N1B4_5313 0.87 +0.0 63,731 + Pf1N1B4_5313 0.87 -2.9 63,731 + Pf1N1B4_5313 0.87 +0.0 63,731 + Pf1N1B4_5313 0.87 +0.0 63,732 - Pf1N1B4_5313 0.88 +0.0 63,732 - Pf1N1B4_5313 0.88 -2.6 63,820 + -2.9 63,821 - +0.0 63,821 - -2.9 63,898 + -4.2 63,899 - -2.1 63,914 + -4.3
Or see this region's nucleotide sequence