Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4717 and Pf1N1B4_4718 are separated by 207 nucleotides Pf1N1B4_4718 and Pf1N1B4_4719 are separated by 52 nucleotides Pf1N1B4_4719 and Pf1N1B4_4720 are separated by 146 nucleotides
Pf1N1B4_4717 - Agmatinase (EC 3.5.3.11), at 5,213,837 to 5,214,796
_4717
Pf1N1B4_4718 - Transcriptional regulator, DeoR family, at 5,215,004 to 5,215,720
_4718
Pf1N1B4_4719 - Electron transfer flavoprotein, beta subunit, at 5,215,773 to 5,216,114
_4719
Pf1N1B4_4720 - AFG1-like ATPase, at 5,216,261 to 5,217,379
_4720
Position (kb) 5215
5216 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5214.545 kb on + strand, within Pf1N1B4_4717 at 5214.721 kb on + strand at 5214.721 kb on + strand at 5214.722 kb on - strand at 5214.767 kb on + strand at 5214.767 kb on + strand at 5214.768 kb on - strand at 5214.768 kb on - strand at 5214.867 kb on - strand at 5214.867 kb on - strand at 5214.967 kb on - strand at 5215.036 kb on - strand at 5215.036 kb on - strand at 5215.036 kb on - strand at 5215.179 kb on - strand at 5215.179 kb on - strand, within Pf1N1B4_4718 at 5215.179 kb on - strand, within Pf1N1B4_4718 at 5216.058 kb on + strand, within Pf1N1B4_4719 at 5216.059 kb on - strand, within Pf1N1B4_4719 at 5216.059 kb on - strand, within Pf1N1B4_4719 at 5216.245 kb on - strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.249 kb on - strand at 5216.249 kb on - strand at 5216.409 kb on - strand, within Pf1N1B4_4720 at 5216.409 kb on - strand, within Pf1N1B4_4720 at 5216.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 5,214,545 + Pf1N1B4_4717 0.74 -4.1 5,214,721 + -0.0 5,214,721 + -2.6 5,214,722 - -3.2 5,214,767 + -3.6 5,214,767 + -2.1 5,214,768 - -1.3 5,214,768 - -3.2 5,214,867 - -2.2 5,214,867 - -1.8 5,214,967 - -0.0 5,215,036 - -0.0 5,215,036 - -3.0 5,215,036 - -0.0 5,215,179 - -3.6 5,215,179 - Pf1N1B4_4718 0.24 -0.0 5,215,179 - Pf1N1B4_4718 0.24 -2.0 5,216,058 + Pf1N1B4_4719 0.83 -0.0 5,216,059 - Pf1N1B4_4719 0.84 -2.2 5,216,059 - Pf1N1B4_4719 0.84 -3.1 5,216,245 - -2.1 5,216,248 + -3.2 5,216,248 + -3.6 5,216,248 + -0.0 5,216,248 + -0.9 5,216,249 - -0.0 5,216,249 - -2.9 5,216,409 - Pf1N1B4_4720 0.13 -1.6 5,216,409 - Pf1N1B4_4720 0.13 -0.0 5,216,409 - -3.5
Or see this region's nucleotide sequence